Restriction map for
pFN21AE6084
(Linear) (Six-base) MAPSORT of: KIEE6084.seq Check: 3528 from: 1 to: 972
KIEE6084.orf
With 182 enzymes: SgfI *
December 5, 2014 13:23 ..
Acc65I G'GTAC_C
Cuts at: 0 395 972
Size: 395 577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 972
Size: 577 395
AflIII A'CryG_T
Cuts at: 0 575 721 972
Size: 575 146 251
Fragments arranged by size:
575 251 146
AhdI GACnn_n'nnGTC
Cuts at: 0 258 972
Size: 258 714
AleI CACnn'nnGTG
Cuts at: 0 778 972
Size: 778 194
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 912 944 972
Size: 912 32 28
Fragments arranged by size:
912 32 28
ApoI r'AATT_y
Cuts at: 0 334 972
Size: 334 638
AvaI C'yCGr_G
Cuts at: 0 73 515 972
Size: 73 442 457
Fragments arranged by size:
457 442 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 210 243 972
Size: 210 33 729
Fragments arranged by size:
729 210 33
BanI G'GyrC_C
Cuts at: 0 395 972
Size: 395 577
BbsI GAAGACnn'nnnn_
Cuts at: 0 7 87 972
Size: 7 80 885
Fragments arranged by size:
885 80 7
BbvCI CC'TCA_GC
Cuts at: 0 695 972
Size: 695 277
BciVI GTATCCnnnnn_n'
Cuts at: 0 204 972
Size: 204 768
BclI T'GATC_A
Cuts at: 0 959 972
Size: 959 13
BglII A'GATC_T
Cuts at: 0 302 972
Size: 302 670
BlpI GC'TnA_GC
Cuts at: 0 190 972
Size: 190 782
BmgBI CAC'GTC
Cuts at: 0 578 972
Size: 578 394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 232 788 972
Size: 4 228 556 184
Fragments arranged by size:
556 228 184 4
Bpu10I CC'TnA_GC
Cuts at: 0 695 972
Size: 695 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 972
Size: 897 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 355 583 972
Size: 355 228 389
Fragments arranged by size:
389 355 228
BsaAI yAC'GTr
Cuts at: 0 722 972
Size: 722 250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 142 172 912 942 972
Size: 142 30 740 30 30
Fragments arranged by size:
740 142 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 972
Size: 394 578
BseYI C'CCAG_C
Cuts at: 0 345 546 972
Size: 345 201 426
Fragments arranged by size:
426 345 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 972
Size: 314 658
BspHI T'CATG_A
Cuts at: 0 134 407 972
Size: 134 273 565
Fragments arranged by size:
565 273 134
BsrFI r'CCGG_y
Cuts at: 0 701 972
Size: 701 271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 422 972
Size: 422 550
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 972
Size: 108 864
BstYI r'GATC_y
Cuts at: 0 302 511 542 972
Size: 302 209 31 430
Fragments arranged by size:
430 302 209 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 232 577 788 972
Size: 4 228 345 211 184
Fragments arranged by size:
345 228 211 184 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 262 972
Size: 262 710
EcoO109I rG'GnC_Cy
Cuts at: 0 252 972
Size: 252 720
EcoRI G'AATT_C
Cuts at: 0 334 972
Size: 334 638
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 244 276 972
Size: 244 32 696
Fragments arranged by size:
696 244 32
KpnI G_GTAC'C
Cuts at: 0 399 972
Size: 399 573
MslI CAynn'nnrTG
Cuts at: 0 106 151 175 739 778 850 946 972
Size: 106 45 24 564 39 72 96 26
Fragments arranged by size:
564 106 96 72 45 39 26 24
NaeI GCC'GGC
Cuts at: 0 703 972
Size: 703 269
NgoMIV G'CCGG_C
Cuts at: 0 701 972
Size: 701 271
PflMI CCAn_nnn'nTGG
Cuts at: 0 738 972
Size: 738 234
PfoI T'CCnGG_A
Cuts at: 0 248 972
Size: 248 724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 912 944 972
Size: 912 32 28
Fragments arranged by size:
912 32 28
PpuMI rG'GwC_Cy
Cuts at: 0 252 972
Size: 252 720
PshAI GACnn'nnGTC
Cuts at: 0 484 972
Size: 484 488
PstI C_TGCA'G
Cuts at: 0 900 972
Size: 900 72
ScaI AGT'ACT
Cuts at: 0 914 972
Size: 914 58
SfcI C'TryA_G
Cuts at: 0 507 870 896 972
Size: 507 363 26 76
Fragments arranged by size:
507 363 76 26
SmaI CCC'GGG
Cuts at: 0 75 972
Size: 75 897
SmlI C'TyrA_G
Cuts at: 0 876 972
Size: 876 96
StuI AGG'CCT
Cuts at: 0 50 972
Size: 50 922
StyI C'CwwG_G
Cuts at: 0 263 461 972
Size: 263 198 511
Fragments arranged by size:
511 263 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 73 189 972
Size: 73 116 783
Fragments arranged by size:
783 116 73
TatI w'GTAC_w
Cuts at: 0 179 912 972
Size: 179 733 60
Fragments arranged by size:
733 179 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 142 174 972
Size: 142 32 798
Fragments arranged by size:
798 142 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 282 972
Size: 108 174 690
Fragments arranged by size:
690 174 108
XmaI C'CCGG_G
Cuts at: 0 73 972
Size: 73 899
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AloI ApoI AvaI
BaeI BanI BbsI BbvCI BciVI BclI BglII BlpI
BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BspHI BsrFI BstAPI BstXI BstYI Eco57MI
EcoNI EcoO109I EcoRI Hin4I KpnI MslI NaeI NgoMIV
PflMI PfoI PpiI PpuMI PshAI PstI ScaI SfcI
SmaI SmlI StuI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BamHI
BanII BbeI BcgI BfrBI BglI Bme1580I BmrI BmtI
BplI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MmeI MscI
MspA1I NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI ZraI