Restriction map for
pFN21AE6083
(Linear) (Six-base) MAPSORT of: KIEE6083.seq Check: 2896 from: 1 to: 972
KIEE6083.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AccI GT'mk_AC
Cuts at: 0 233 972
Size: 233 739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 889 972
Size: 740 149 83
Fragments arranged by size:
740 149 83
AfeI AGC'GCT
Cuts at: 0 316 972
Size: 316 656
AleI CACnn'nnGTG
Cuts at: 0 499 972
Size: 499 473
AlwNI CAG_nnn'CTG
Cuts at: 0 505 972
Size: 505 467
ApoI r'AATT_y
Cuts at: 0 43 972
Size: 43 929
AvaI C'yCGr_G
Cuts at: 0 78 972
Size: 78 894
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 112 145 972
Size: 112 33 827
Fragments arranged by size:
827 112 33
BciVI GTATCCnnnnn_n'
Cuts at: 0 376 972
Size: 376 596
BglII A'GATC_T
Cuts at: 0 967 972
Size: 967 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 972
Size: 482 490
Bpu10I CC'TnA_GC
Cuts at: 0 311 739 972
Size: 311 428 233
Fragments arranged by size:
428 311 233
BsaI GGTCTCn'nnnn_
Cuts at: 0 57 972
Size: 57 915
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 361 972
Size: 361 611
BseYI C'CCAG_C
Cuts at: 0 69 972
Size: 69 903
BsiHKAI G_wGCw'C
Cuts at: 0 565 745 972
Size: 565 180 227
Fragments arranged by size:
565 227 180
BsmI GAATG_Cn'
Cuts at: 0 83 972
Size: 83 889
Bsp1286I G_dGCh'C
Cuts at: 0 565 745 972
Size: 565 180 227
Fragments arranged by size:
565 227 180
BstAPI GCAn_nnn'nTGC
Cuts at: 0 145 972
Size: 145 827
BstYI r'GATC_y
Cuts at: 0 266 967 972
Size: 266 701 5
Fragments arranged by size:
701 266 5
BtsI GCAGTG_nn'
Cuts at: 0 552 972
Size: 552 420
DraIII CAC_nnn'GTG
Cuts at: 0 523 972
Size: 523 449
EaeI y'GGCC_r
Cuts at: 0 245 272 972
Size: 245 27 700
Fragments arranged by size:
700 245 27
EarI CTCTTCn'nnn_
Cuts at: 0 419 972
Size: 419 553
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 740 889 972
Size: 482 258 149 83
Fragments arranged by size:
482 258 149 83
EcoO109I rG'GnC_Cy
Cuts at: 0 735 972
Size: 735 237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 605 972
Size: 605 367
HaeII r_GCGC'y
Cuts at: 0 318 972
Size: 318 654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 972
Size: 517 32 423
Fragments arranged by size:
517 423 32
MfeI C'AATT_G
Cuts at: 0 428 972
Size: 428 544
MscI TGG'CCA
Cuts at: 0 247 274 972
Size: 247 27 698
Fragments arranged by size:
698 247 27
MslI CAynn'nnrTG
Cuts at: 0 499 972
Size: 499 473
MspA1I CmG'CkG
Cuts at: 0 92 718 972
Size: 92 626 254
Fragments arranged by size:
626 254 92
NdeI CA'TA_TG
Cuts at: 0 8 972
Size: 8 964
PflMI CCAn_nnn'nTGG
Cuts at: 0 281 972
Size: 281 691
PshAI GACnn'nnGTC
Cuts at: 0 239 972
Size: 239 733
PstI C_TGCA'G
Cuts at: 0 729 972
Size: 729 243
PvuII CAG'CTG
Cuts at: 0 92 718 972
Size: 92 626 254
Fragments arranged by size:
626 254 92
SfcI C'TryA_G
Cuts at: 0 725 890 972
Size: 725 165 82
Fragments arranged by size:
725 165 82
SmaI CCC'GGG
Cuts at: 0 80 972
Size: 80 892
StyI C'CwwG_G
Cuts at: 0 213 293 570 731 972
Size: 213 80 277 161 241
Fragments arranged by size:
277 241 213 161 80
TatI w'GTAC_w
Cuts at: 0 692 699 972
Size: 692 7 273
Fragments arranged by size:
692 273 7
XmaI C'CCGG_G
Cuts at: 0 78 972
Size: 78 894
Enzymes that do cut:
AccI AcuI AfeI AleI AlwNI ApoI AvaI BaeI
BciVI BglII BpmI Bpu10I BsaI BseRI BseYI BsiHKAI
BsmI Bsp1286I BstAPI BstYI BtsI DraIII EaeI EarI
Eco57MI EcoO109I FalI HaeII Hin4I MfeI MscI MslI
MspA1I NdeI PflMI PshAI PstI PvuII SfcI SmaI
StyI TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MluI MmeI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI
ZraI