Restriction map for
pFN21AE6078
(Linear) (Six-base) MAPSORT of: KIEE6078.seq Check: 4733 from: 1 to: 969
KIEE6078.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AccI GT'mk_AC
Cuts at: 0 226 969
Size: 226 743
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 969
Size: 514 455
AflIII A'CryG_T
Cuts at: 0 816 969
Size: 816 153
AgeI A'CCGG_T
Cuts at: 0 920 969
Size: 920 49
AleI CACnn'nnGTG
Cuts at: 0 235 740 969
Size: 235 505 229
Fragments arranged by size:
505 235 229
BanII G_rGCy'C
Cuts at: 0 967 969
Size: 967 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 895 969
Size: 895 74
BbvCI CC'TCA_GC
Cuts at: 0 718 969
Size: 718 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 446 480 969
Size: 446 34 489
Fragments arranged by size:
489 446 34
Bme1580I G_kGCm'C
Cuts at: 0 179 242 390 614 969
Size: 179 63 148 224 355
Fragments arranged by size:
355 224 179 148 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 472 969
Size: 472 497
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 455 969
Size: 455 514
Bpu10I CC'TnA_GC
Cuts at: 0 718 969
Size: 718 251
BsaAI yAC'GTr
Cuts at: 0 904 969
Size: 904 65
BsaWI w'CCGG_w
Cuts at: 0 920 969
Size: 920 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 653 683 969
Size: 653 30 286
Fragments arranged by size:
653 286 30
Bsp1286I G_dGCh'C
Cuts at: 0 179 242 390 614 967 969
Size: 179 63 148 224 353 2
Fragments arranged by size:
353 224 179 148 63 2
BsrDI GCAATG_nn'
Cuts at: 0 278 969
Size: 278 691
BsrFI r'CCGG_y
Cuts at: 0 920 969
Size: 920 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 621 796 969
Size: 621 175 173
Fragments arranged by size:
621 175 173
BtgI C'CryG_G
Cuts at: 0 356 969
Size: 356 613
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 630 969
Size: 630 339
BtsI GCAGTG_nn'
Cuts at: 0 113 683 857 969
Size: 113 570 174 112
Fragments arranged by size:
570 174 113 112
EaeI y'GGCC_r
Cuts at: 0 203 377 443 833 969
Size: 203 174 66 390 136
Fragments arranged by size:
390 203 174 136 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 969
Size: 514 455
EcoO109I rG'GnC_Cy
Cuts at: 0 246 714 969
Size: 246 468 255
Fragments arranged by size:
468 255 246
HincII GTy'rAC
Cuts at: 0 588 969
Size: 588 381
HindIII A'AGCT_T
Cuts at: 0 459 969
Size: 459 510
HpaI GTT'AAC
Cuts at: 0 588 969
Size: 588 381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 969
Size: 44 925
MscI TGG'CCA
Cuts at: 0 379 835 969
Size: 379 456 134
Fragments arranged by size:
456 379 134
MslI CAynn'nnrTG
Cuts at: 0 11 235 619 649 740 751 969
Size: 11 224 384 30 91 11 218
Fragments arranged by size:
384 224 218 91 30 11 11
NcoI C'CATG_G
Cuts at: 0 356 969
Size: 356 613
NdeI CA'TA_TG
Cuts at: 0 320 969
Size: 320 649
NspI r_CATG'y
Cuts at: 0 820 969
Size: 820 149
PasI CC'CwG_GG
Cuts at: 0 58 969
Size: 58 911
PciI A'CATG_T
Cuts at: 0 816 969
Size: 816 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 260 969
Size: 260 709
PstI C_TGCA'G
Cuts at: 0 36 120 969
Size: 36 84 849
Fragments arranged by size:
849 84 36
SfcI C'TryA_G
Cuts at: 0 32 116 969
Size: 32 84 853
Fragments arranged by size:
853 84 32
SspI AAT'ATT
Cuts at: 0 807 969
Size: 807 162
StyI C'CwwG_G
Cuts at: 0 242 303 356 969
Size: 242 61 53 613
Fragments arranged by size:
613 242 61 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 969
Size: 316 653
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 653 685 969
Size: 653 32 284
Fragments arranged by size:
653 284 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 843 969
Size: 843 126
Enzymes that do cut:
AccI AcuI AflIII AgeI AleI BanII BbsI BbvCI
BcgI Bme1580I BmrI BplI Bpu10I BsaAI BsaWI BsaXI
Bsp1286I BsrDI BsrFI BstXI BtgI BtgZI BtsI EaeI
Eco57MI EcoO109I HincII HindIII HpaI MmeI MscI MslI
NcoI NdeI NspI PasI PciI PflMI PstI SfcI
SspI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BpmI BpuEI
BsaI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I KasI KpnI MfeI MluI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI