(Linear) (Six-base) MAPSORT of: KIEE6077.seq  Check: 3632  from: 1  to: 969



KIEE6077.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:22  ..

AccI GT'mk_AC

Cuts at:      0     125     969 
   Size:        125     844
 
AfeI AGC'GCT

Cuts at:      0     425     969 
   Size:        425     544
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     709     969 
   Size:        709     260
 
ApaLI G'TGCA_C

Cuts at:      0      89     969 
   Size:         89     880
 
BanI G'GyrC_C

Cuts at:      0     151     969 
   Size:        151     818
 
BbvCI CC'TCA_GC

Cuts at:      0     306     969 
   Size:        306     663
 
BglI GCCn_nnn'nGGC

Cuts at:      0     683     969 
   Size:        683     286
 
Bme1580I G_kGCm'C

Cuts at:      0      93     393     969 
   Size:         93     300     576

  Fragments arranged by size: 

                 576     300      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     151     969 
   Size:        151     818
 
Bpu10I CC'TnA_GC

Cuts at:      0     306     812     969 
   Size:        306     506     157

  Fragments arranged by size: 

                 506     306     157
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     490     969 
   Size:        490     479
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     418     757     969 
   Size:        418     339     212

  Fragments arranged by size: 

                 418     339     212
 
BseYI C'CCAG_C

Cuts at:      0     267     420     969 
   Size:        267     153     549

  Fragments arranged by size: 

                 549     267     153
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     172     626     969 
   Size:        122      50     454     343

  Fragments arranged by size: 

                 454     343     122      50
 
BsiHKAI G_wGCw'C

Cuts at:      0      93     291     767     966     969 
   Size:         93     198     476     199       3

  Fragments arranged by size: 

                 476     199     198      93       3
 
Bsp1286I G_dGCh'C

Cuts at:      0      93     291     393     767     966     969 
   Size:         93     198     102     374     199       3

  Fragments arranged by size: 

                 374     199     198     102      93       3
 
BsrDI GCAATG_nn'

Cuts at:      0     106     116     167     969 
   Size:        106      10      51     802

  Fragments arranged by size: 

                 802     106      51      10
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     267     599     645     954     969 
   Size:        267     332      46     309      15

  Fragments arranged by size: 

                 332     309     267      46      15
 
BtgI C'CryG_G

Cuts at:      0     638     969 
   Size:        638     331
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     258     969 
   Size:        258     711
 
BtsI GCAGTG_nn'

Cuts at:      0     245     262     767     969 
   Size:        245      17     505     202

  Fragments arranged by size: 

                 505     245     202      17
 
EaeI y'GGCC_r

Cuts at:      0     395     461     969 
   Size:        395      66     508

  Fragments arranged by size: 

                 508     395      66
 
EarI CTCTTCn'nnn_

Cuts at:      0     217     775     848     969 
   Size:        217     558      73     121

  Fragments arranged by size: 

                 558     217     121      73
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     535     969 
   Size:        535     434
 
EcoO109I rG'GnC_Cy

Cuts at:      0     540     969 
   Size:        540     429
 
HaeII r_GCGC'y

Cuts at:      0     427     969 
   Size:        427     542
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     792     824     969 
   Size:        792      32     145

  Fragments arranged by size: 

                 792     145      32
 
HincII GTy'rAC

Cuts at:      0       5     969 
   Size:          5     964
 
HindIII A'AGCT_T

Cuts at:      0     596     969 
   Size:        596     373
 
MscI TGG'CCA

Cuts at:      0     397     463     969 
   Size:        397      66     506

  Fragments arranged by size: 

                 506     397      66
 
MslI CAynn'nnrTG

Cuts at:      0      43     569     969 
   Size:         43     526     400

  Fragments arranged by size: 

                 526     400      43
 
NcoI C'CATG_G

Cuts at:      0     638     969 
   Size:        638     331
 
NspI r_CATG'y

Cuts at:      0      75     522     969 
   Size:         75     447     447

  Fragments arranged by size: 

                 447     447      75
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     161     969 
   Size:        161     808
 
PpuMI rG'GwC_Cy

Cuts at:      0     540     969 
   Size:        540     429
 
PstI C_TGCA'G

Cuts at:      0     179     762     969 
   Size:        179     583     207

  Fragments arranged by size: 

                 583     207     179
 
SfcI C'TryA_G

Cuts at:      0     175     319     612     758     894     969 
   Size:        175     144     293     146     136      75

  Fragments arranged by size: 

                 293     175     146     144     136      75
 
SphI G_CATG'C

Cuts at:      0      75     969 
   Size:         75     894
 
StuI AGG'CCT

Cuts at:      0      80     969 
   Size:         80     889
 
StyI C'CwwG_G

Cuts at:      0     638     969 
   Size:        638     331
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     946     969 
   Size:        946      23
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     942     969 
   Size:        942      27
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      60     969 
   Size:         60     909
 

 Enzymes that do cut:

     AccI     AfeI     AlfI    ApaLI     BanI    BbvCI     BglI Bme1580I
     BmrI   Bpu10I     BsaI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BsrDI    BstXI     BtgI    BtgZI     BtsI     EaeI     EarI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII  HindIII     MscI     MslI     NcoI
     NspI    PflMI    PpuMI     PstI     SfcI     SphI     StuI     StyI
    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI