Restriction map for
pFN21AE6075
(Linear) (Six-base) MAPSORT of: KIEE6075.seq Check: 5960 from: 1 to: 969
KIEE6075.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AccI GT'mk_AC
Cuts at: 0 521 969
Size: 521 448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 969
Size: 256 713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 817 969
Size: 817 152
ApaLI G'TGCA_C
Cuts at: 0 792 969
Size: 792 177
ApoI r'AATT_y
Cuts at: 0 852 969
Size: 852 117
AvaI C'yCGr_G
Cuts at: 0 962 969
Size: 962 7
BanI G'GyrC_C
Cuts at: 0 754 969
Size: 754 215
BanII G_rGCy'C
Cuts at: 0 50 969
Size: 50 919
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 645 969
Size: 93 552 324
Fragments arranged by size:
552 324 93
BbvCI CC'TCA_GC
Cuts at: 0 910 969
Size: 910 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 471 505 969
Size: 471 34 464
Fragments arranged by size:
471 464 34
BfrBI ATG'CAT
Cuts at: 0 216 969
Size: 216 753
BlpI GC'TnA_GC
Cuts at: 0 187 969
Size: 187 782
Bme1580I G_kGCm'C
Cuts at: 0 796 969
Size: 796 173
BmrI ACTGGGnnnn_n'
Cuts at: 0 783 924 969
Size: 783 141 45
Fragments arranged by size:
783 141 45
Bpu10I CC'TnA_GC
Cuts at: 0 890 910 969
Size: 890 20 59
Fragments arranged by size:
890 59 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 969
Size: 61 908
BsaI GGTCTCn'nnnn_
Cuts at: 0 580 771 969
Size: 580 191 198
Fragments arranged by size:
580 198 191
BsaWI w'CCGG_w
Cuts at: 0 872 969
Size: 872 97
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 604 607 610 927 969
Size: 130 474 3 3 317 42
Fragments arranged by size:
474 317 130 42 3 3
BseYI C'CCAG_C
Cuts at: 0 158 296 543 969
Size: 158 138 247 426
Fragments arranged by size:
426 247 158 138
BsiHKAI G_wGCw'C
Cuts at: 0 404 796 969
Size: 404 392 173
Fragments arranged by size:
404 392 173
BsmI GAATG_Cn'
Cuts at: 0 608 935 969
Size: 608 327 34
Fragments arranged by size:
608 327 34
Bsp1286I G_dGCh'C
Cuts at: 0 50 404 796 969
Size: 50 354 392 173
Fragments arranged by size:
392 354 173 50
BspEI T'CCGG_A
Cuts at: 0 872 969
Size: 872 97
BspHI T'CATG_A
Cuts at: 0 404 969
Size: 404 565
BsrGI T'GTAC_A
Cuts at: 0 596 969
Size: 596 373
BstYI r'GATC_y
Cuts at: 0 207 969
Size: 207 762
BtgI C'CryG_G
Cuts at: 0 92 969
Size: 92 877
BtsI GCAGTG_nn'
Cuts at: 0 385 969
Size: 385 584
DraIII CAC_nnn'GTG
Cuts at: 0 932 969
Size: 932 37
EarI CTCTTCn'nnn_
Cuts at: 0 55 897 969
Size: 55 842 72
Fragments arranged by size:
842 72 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 969
Size: 256 713
EcoO109I rG'GnC_Cy
Cuts at: 0 785 969
Size: 785 184
EcoRI G'AATT_C
Cuts at: 0 852 969
Size: 852 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 852 884 908 940 969
Size: 852 32 24 32 29
Fragments arranged by size:
852 32 32 29 24
MfeI C'AATT_G
Cuts at: 0 949 969
Size: 949 20
MslI CAynn'nnrTG
Cuts at: 0 577 676 969
Size: 577 99 293
Fragments arranged by size:
577 293 99
MspA1I CmG'CkG
Cuts at: 0 547 969
Size: 547 422
NcoI C'CATG_G
Cuts at: 0 92 969
Size: 92 877
NsiI A_TGCA'T
Cuts at: 0 218 969
Size: 218 751
NspI r_CATG'y
Cuts at: 0 220 969
Size: 220 749
PpuMI rG'GwC_Cy
Cuts at: 0 785 969
Size: 785 184
PshAI GACnn'nnGTC
Cuts at: 0 783 969
Size: 783 186
PvuII CAG'CTG
Cuts at: 0 547 969
Size: 547 422
SapI GCTCTTCn'nnn_
Cuts at: 0 55 969
Size: 55 914
SfcI C'TryA_G
Cuts at: 0 828 969
Size: 828 141
SmlI C'TyrA_G
Cuts at: 0 40 969
Size: 40 929
StyI C'CwwG_G
Cuts at: 0 92 969
Size: 92 877
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 943 969
Size: 943 26
TatI w'GTAC_w
Cuts at: 0 596 969
Size: 596 373
XmnI GAAnn'nnTTC
Cuts at: 0 335 969
Size: 335 634
Enzymes that do cut:
AccI AcuI AlfI ApaLI ApoI AvaI BanI BanII
BbsI BbvCI BcgI BfrBI BlpI Bme1580I BmrI Bpu10I
BpuEI BsaI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspEI BspHI BsrGI BstYI BtgI BtsI DraIII EarI
Eco57MI EcoO109I EcoRI Hin4I MfeI MslI MspA1I NcoI
NsiI NspI PpuMI PshAI PvuII SapI SfcI SmlI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BciVI BclI BglI BglII
BmgBI BmtI BplI BpmI BsaAI BsaBI BsaHI BsaXI
BsgI BsiEI BsiWI BsmBI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI