Restriction map for
pFN21AE6073
(Linear) (Six-base) MAPSORT of: KIEE6073.seq Check: 9232 from: 1 to: 963
KIEE6073.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 963
Size: 812 151
AflIII A'CryG_T
Cuts at: 0 745 963
Size: 745 218
AlwNI CAG_nnn'CTG
Cuts at: 0 31 963
Size: 31 932
BanII G_rGCy'C
Cuts at: 0 185 963
Size: 185 778
BbvCI CC'TCA_GC
Cuts at: 0 154 963
Size: 154 809
BmrI ACTGGGnnnn_n'
Cuts at: 0 79 152 820 963
Size: 79 73 668 143
Fragments arranged by size:
668 143 79 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 963
Size: 579 384
Bpu10I CC'TnA_GC
Cuts at: 0 154 963
Size: 154 809
BsaAI yAC'GTr
Cuts at: 0 687 746 963
Size: 687 59 217
Fragments arranged by size:
687 217 59
BsaHI Gr'CG_yC
Cuts at: 0 311 963
Size: 311 652
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 109 835 963
Size: 106 3 726 128
Fragments arranged by size:
726 128 106 3
BseYI C'CCAG_C
Cuts at: 0 31 323 349 963
Size: 31 292 26 614
Fragments arranged by size:
614 292 31 26
BsiHKAI G_wGCw'C
Cuts at: 0 116 927 963
Size: 116 811 36
Fragments arranged by size:
811 116 36
BsmBI CGTCTCn'nnnn_
Cuts at: 0 283 963
Size: 283 680
Bsp1286I G_dGCh'C
Cuts at: 0 116 185 927 963
Size: 116 69 742 36
Fragments arranged by size:
742 116 69 36
BsrDI GCAATG_nn'
Cuts at: 0 272 963
Size: 272 691
BtgI C'CryG_G
Cuts at: 0 377 764 963
Size: 377 387 199
Fragments arranged by size:
387 377 199
BtsI GCAGTG_nn'
Cuts at: 0 671 963
Size: 671 292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 513 548 963
Size: 513 35 415
Fragments arranged by size:
513 415 35
DraIII CAC_nnn'GTG
Cuts at: 0 507 963
Size: 507 456
EaeI y'GGCC_r
Cuts at: 0 398 509 963
Size: 398 111 454
Fragments arranged by size:
454 398 111
EarI CTCTTCn'nnn_
Cuts at: 0 83 334 583 780 963
Size: 83 251 249 197 183
Fragments arranged by size:
251 249 197 183 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 812 963
Size: 579 233 151
Fragments arranged by size:
579 233 151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 753 963
Size: 753 210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 828 963
Size: 219 609 135
Fragments arranged by size:
609 219 135
MscI TGG'CCA
Cuts at: 0 400 511 963
Size: 400 111 452
Fragments arranged by size:
452 400 111
MslI CAynn'nnrTG
Cuts at: 0 430 963
Size: 430 533
MspA1I CmG'CkG
Cuts at: 0 49 694 963
Size: 49 645 269
Fragments arranged by size:
645 269 49
NcoI C'CATG_G
Cuts at: 0 377 963
Size: 377 586
PflMI CCAn_nnn'nTGG
Cuts at: 0 31 345 507 963
Size: 31 314 162 456
Fragments arranged by size:
456 314 162 31
PmlI CAC'GTG
Cuts at: 0 687 746 963
Size: 687 59 217
Fragments arranged by size:
687 217 59
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 798 830 963
Size: 798 32 133
Fragments arranged by size:
798 133 32
PstI C_TGCA'G
Cuts at: 0 699 963
Size: 699 264
PvuII CAG'CTG
Cuts at: 0 694 963
Size: 694 269
SapI GCTCTTCn'nnn_
Cuts at: 0 334 583 963
Size: 334 249 380
Fragments arranged by size:
380 334 249
SfcI C'TryA_G
Cuts at: 0 420 678 695 894 963
Size: 420 258 17 199 69
Fragments arranged by size:
420 258 199 69 17
SspI AAT'ATT
Cuts at: 0 938 963
Size: 938 25
StuI AGG'CCT
Cuts at: 0 736 963
Size: 736 227
StyI C'CwwG_G
Cuts at: 0 377 470 963
Size: 377 93 493
Fragments arranged by size:
493 377 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 404 616 963
Size: 404 212 347
Fragments arranged by size:
404 347 212
TatI w'GTAC_w
Cuts at: 0 203 963
Size: 203 760
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 563 595 963
Size: 563 32 368
Fragments arranged by size:
563 368 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 346 963
Size: 346 617
XmnI GAAnn'nnTTC
Cuts at: 0 711 736 963
Size: 711 25 227
Fragments arranged by size:
711 227 25
Enzymes that do cut:
AcuI AflIII AlwNI BanII BbvCI BmrI BpmI Bpu10I
BsaAI BsaHI BseRI BseYI BsiHKAI BsmBI Bsp1286I BsrDI
BtgI BtsI CspCI DraIII EaeI EarI Eco57MI FalI
MmeI MscI MslI MspA1I NcoI PflMI PmlI PpiI
PstI PvuII SapI SfcI SspI StuI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI BpuEI BsaI BsaBI BsaWI
BsaXI BsgI BsiEI BsiWI BsmI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI DraI DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI