(Linear) (Six-base) MAPSORT of: KIEE6072.seq  Check: 6111  from: 1  to: 963



KIEE6072.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:22  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     963 
   Size:         88     875
 
AleI CACnn'nnGTG

Cuts at:      0     115     963 
   Size:        115     848
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     403     963 
   Size:        403     560
 
ApoI r'AATT_y

Cuts at:      0     919     963 
   Size:        919      44
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     376     409     963 
   Size:        376      33     554

  Fragments arranged by size: 

                 554     376      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      76     154     963 
   Size:         76      78     809

  Fragments arranged by size: 

                 809      78      76
 
BclI T'GATC_A

Cuts at:      0     107     963 
   Size:        107     856
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      33     963 
   Size:         33     930
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     515     963 
   Size:        515     448
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     803     963 
   Size:        803     160
 
BsmI GAATG_Cn'

Cuts at:      0     422     963 
   Size:        422     541
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     467     963 
   Size:        467     496
 
BsrDI GCAATG_nn'

Cuts at:      0     356     963 
   Size:        356     607
 
BsrGI T'GTAC_A

Cuts at:      0     176     963 
   Size:        176     787
 
BtgI C'CryG_G

Cuts at:      0     113     963 
   Size:        113     850
 
BtsI GCAGTG_nn'

Cuts at:      0     385     963 
   Size:        385     578
 
EaeI y'GGCC_r

Cuts at:      0     371     963 
   Size:        371     592
 
EarI CTCTTCn'nnn_

Cuts at:      0     687     766     907     963 
   Size:        687      79     141      56

  Fragments arranged by size: 

                 687     141      79      56
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     963 
   Size:         88     875
 
HincII GTy'rAC

Cuts at:      0     497     963 
   Size:        497     466
 
HindIII A'AGCT_T

Cuts at:      0     701     963 
   Size:        701     262
 
HpaI GTT'AAC

Cuts at:      0     497     963 
   Size:        497     466
 
MscI TGG'CCA

Cuts at:      0     373     963 
   Size:        373     590
 
MslI CAynn'nnrTG

Cuts at:      0     115     172     403     427     963 
   Size:        115      57     231      24     536

  Fragments arranged by size: 

                 536     231     115      57      24
 
NcoI C'CATG_G

Cuts at:      0     113     963 
   Size:        113     850
 
NdeI CA'TA_TG

Cuts at:      0     376     963 
   Size:        376     587
 
PstI C_TGCA'G

Cuts at:      0     392     438     963 
   Size:        392      46     525

  Fragments arranged by size: 

                 525     392      46
 
SexAI A'CCwGG_T

Cuts at:      0     777     963 
   Size:        777     186
 
SfcI C'TryA_G

Cuts at:      0     326     388     434     629     963 
   Size:        326      62      46     195     334

  Fragments arranged by size: 

                 334     326     195      62      46
 
SspI AAT'ATT

Cuts at:      0     668     743     891     963 
   Size:        668      75     148      72

  Fragments arranged by size: 

                 668     148      75      72
 
StyI C'CwwG_G

Cuts at:      0     113     683     963 
   Size:        113     570     280

  Fragments arranged by size: 

                 570     280     113
 
TatI w'GTAC_w

Cuts at:      0     176     963 
   Size:        176     787
 

 Enzymes that do cut:

     AcuI     AleI     AlfI     ApoI     BaeI     BbsI     BclI     BplI
     BsaI     BsgI     BsmI    BspMI    BsrDI    BsrGI     BtgI     BtsI
     EaeI     EarI  Eco57MI   HincII  HindIII     HpaI     MscI     MslI
     NcoI     NdeI     PstI    SexAI     SfcI     SspI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI