(Linear) (Six-base) MAPSORT of: KIEE6069.seq  Check: 8281  from: 1  to: 963



KIEE6069.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:22  ..

AflIII A'CryG_T

Cuts at:      0     548     963 
   Size:        548     415
 
AhdI GACnn_n'nnGTC

Cuts at:      0     549     963 
   Size:        549     414
 
AleI CACnn'nnGTG

Cuts at:      0     740     963 
   Size:        740     223
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     794     826     963 
   Size:        794      32     137

  Fragments arranged by size: 

                 794     137      32
 
BamHI G'GATC_C

Cuts at:      0     923     963 
   Size:        923      40
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      74     963 
   Size:         74     889
 
BbvCI CC'TCA_GC

Cuts at:      0     693     963 
   Size:        693     270
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     936     963 
   Size:        936      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     749     963 
   Size:        749     214
 
Bpu10I CC'TnA_GC

Cuts at:      0     693     963 
   Size:        693     270
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     963 
   Size:        256     707
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     130     681     963 
   Size:        130     551     282

  Fragments arranged by size: 

                 551     282     130
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     711     963 
   Size:        154     557     252

  Fragments arranged by size: 

                 557     252     154
 
BsiHKAI G_wGCw'C

Cuts at:      0     229     963 
   Size:        229     734
 
BsmI GAATG_Cn'

Cuts at:      0     298     963 
   Size:        298     665
 
Bsp1286I G_dGCh'C

Cuts at:      0     229     963 
   Size:        229     734
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     733     963 
   Size:        733     230
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     796     963 
   Size:        796     167
 
BstYI r'GATC_y

Cuts at:      0     923     963 
   Size:        923      40
 
BtsI GCAGTG_nn'

Cuts at:      0     683     728     963 
   Size:        683      45     235

  Fragments arranged by size: 

                 683     235      45
 
EaeI y'GGCC_r

Cuts at:      0     194     377     963 
   Size:        194     183     586

  Fragments arranged by size: 

                 586     194     183
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     749     963 
   Size:        749     214
 
HaeII r_GCGC'y

Cuts at:      0     501     963 
   Size:        501     462
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     535     567     899     931     934     963 
   Size:        535      32     332      32       3      29

  Fragments arranged by size: 

                 535     332      32      32      29       3
 
MscI TGG'CCA

Cuts at:      0     196     379     963 
   Size:        196     183     584

  Fragments arranged by size: 

                 584     196     183
 
MslI CAynn'nnrTG

Cuts at:      0     331     740     963 
   Size:        331     409     223

  Fragments arranged by size: 

                 409     331     223
 
NspI r_CATG'y

Cuts at:      0     552     963 
   Size:        552     411
 
PciI A'CATG_T

Cuts at:      0     548     963 
   Size:        548     415
 
PstI C_TGCA'G

Cuts at:      0     514     963 
   Size:        514     449
 
SfcI C'TryA_G

Cuts at:      0     510     554     954     963 
   Size:        510      44     400       9

  Fragments arranged by size: 

                 510     400      44       9
 
SmlI C'TyrA_G

Cuts at:      0     271     963 
   Size:        271     692
 
SspI AAT'ATT

Cuts at:      0     843     963 
   Size:        843     120
 
StuI AGG'CCT

Cuts at:      0     715     963 
   Size:        715     248
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     876     963 
   Size:        876      87
 
TatI w'GTAC_w

Cuts at:      0     182     232     963 
   Size:        182      50     731

  Fragments arranged by size: 

                 731     182      50
 
Tth111I GACn'n_nGTC

Cuts at:      0     913     963 
   Size:        913      50
 

 Enzymes that do cut:

   AflIII     AhdI     AleI     AloI    BamHI     BbsI    BbvCI    BciVI
     BpmI   Bpu10I    BpuEI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspMI    BstXI    BstYI     BtsI     EaeI  Eco57MI    HaeII    Hin4I
     MscI     MslI     NspI     PciI     PstI     SfcI     SmlI     SspI
     StuI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StyI
     SwaI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI