Restriction map for
pFN21AE6068
(Linear) (Six-base) MAPSORT of: KIEE6068.seq Check: 450 from: 1 to: 963
KIEE6068.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 733 963
Size: 733 230
Acc65I G'GTAC_C
Cuts at: 0 237 963
Size: 237 726
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 963
Size: 702 261
AflIII A'CryG_T
Cuts at: 0 737 963
Size: 737 226
AhdI GACnn_n'nnGTC
Cuts at: 0 549 963
Size: 549 414
AlwNI CAG_nnn'CTG
Cuts at: 0 725 963
Size: 725 238
ApaLI G'TGCA_C
Cuts at: 0 225 963
Size: 225 738
ApoI r'AATT_y
Cuts at: 0 922 963
Size: 922 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 221 254 963
Size: 221 33 709
Fragments arranged by size:
709 221 33
BanI G'GyrC_C
Cuts at: 0 237 963
Size: 237 726
BanII G_rGCy'C
Cuts at: 0 140 963
Size: 140 823
BciVI GTATCCnnnnn_n'
Cuts at: 0 576 963
Size: 576 387
BclI T'GATC_A
Cuts at: 0 752 963
Size: 752 211
BglI GCCn_nnn'nGGC
Cuts at: 0 298 963
Size: 298 665
Bme1580I G_kGCm'C
Cuts at: 0 229 963
Size: 229 734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 963
Size: 3 960
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 474 963
Size: 256 218 489
Fragments arranged by size:
489 256 218
BsaBI GATnn'nnATC
Cuts at: 0 469 963
Size: 469 494
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 926 956 963
Size: 1 30 895 30 7
Fragments arranged by size:
895 30 30 7 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 478 963
Size: 175 303 485
Fragments arranged by size:
485 303 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 711 963
Size: 154 557 252
Fragments arranged by size:
557 252 154
BsiHKAI G_wGCw'C
Cuts at: 0 229 350 963
Size: 229 121 613
Fragments arranged by size:
613 229 121
Bsp1286I G_dGCh'C
Cuts at: 0 140 229 350 963
Size: 140 89 121 613
Fragments arranged by size:
613 140 121 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 963
Size: 733 230
BsrDI GCAATG_nn'
Cuts at: 0 113 963
Size: 113 850
BsrFI r'CCGG_y
Cuts at: 0 173 963
Size: 173 790
BsrGI T'GTAC_A
Cuts at: 0 107 963
Size: 107 856
BstYI r'GATC_y
Cuts at: 0 83 963
Size: 83 880
BtgI C'CryG_G
Cuts at: 0 233 963
Size: 233 730
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 411 963
Size: 411 552
DraIII CAC_nnn'GTG
Cuts at: 0 224 963
Size: 224 739
EaeI y'GGCC_r
Cuts at: 0 377 557 836 963
Size: 377 180 279 127
Fragments arranged by size:
377 279 180 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 702 963
Size: 3 699 261
Fragments arranged by size:
699 261 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 453 963
Size: 453 510
EcoO109I rG'GnC_Cy
Cuts at: 0 165 963
Size: 165 798
EcoRV GAT'ATC
Cuts at: 0 636 963
Size: 636 327
HaeII r_GCGC'y
Cuts at: 0 501 963
Size: 501 462
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1 26 33 58 535 567 597 629
Size: 1 25 7 25 477 32 30 32
Cuts at: 629 794 826 963
Size: 165 32 137
Fragments arranged by size:
477 165 137 32 32 32 30 25
25 7 1
KpnI G_GTAC'C
Cuts at: 0 241 963
Size: 241 722
MfeI C'AATT_G
Cuts at: 0 875 963
Size: 875 88
MscI TGG'CCA
Cuts at: 0 379 559 838 963
Size: 379 180 279 125
Fragments arranged by size:
379 279 180 125
MslI CAynn'nnrTG
Cuts at: 0 232 355 832 963
Size: 232 123 477 131
Fragments arranged by size:
477 232 131 123
NcoI C'CATG_G
Cuts at: 0 233 963
Size: 233 730
NdeI CA'TA_TG
Cuts at: 0 320 548 963
Size: 320 228 415
Fragments arranged by size:
415 320 228
NspI r_CATG'y
Cuts at: 0 741 963
Size: 741 222
PciI A'CATG_T
Cuts at: 0 737 963
Size: 737 226
SmlI C'TyrA_G
Cuts at: 0 271 453 963
Size: 271 182 510
Fragments arranged by size:
510 271 182
StyI C'CwwG_G
Cuts at: 0 233 431 560 963
Size: 233 198 129 403
Fragments arranged by size:
403 233 198 129
TatI w'GTAC_w
Cuts at: 0 107 182 963
Size: 107 75 781
Fragments arranged by size:
781 107 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 727 759 963
Size: 727 32 204
Fragments arranged by size:
727 204 32
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AlwNI ApaLI ApoI
BaeI BanI BanII BciVI BclI BglI Bme1580I BpmI
BpuEI BsaBI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstYI BtgI BtgZI DraIII EaeI
Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KpnI MfeI
MscI MslI NcoI NdeI NspI PciI SmlI StyI
TatI TstI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AlfI
AloI ApaI AscI AseI AsiSI AvaI AvrII BamHI
BbeI BbsI BbvCI BcgI BfrBI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaWI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoRI FalI FseI FspI FspAI HincII HindIII
HpaI KasI MluI MmeI MspA1I NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI