(Linear) (Six-base) MAPSORT of: KIEE6066.seq  Check: 6233  from: 1  to: 960



KIEE6066.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:21  ..

AccI GT'mk_AC

Cuts at:      0     896     960 
   Size:        896      64
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     701     960 
   Size:        221     480     259

  Fragments arranged by size: 

                 480     259     221
 
AlwNI CAG_nnn'CTG

Cuts at:      0     244     960 
   Size:        244     716
 
BanI G'GyrC_C

Cuts at:      0     549     960 
   Size:        549     411
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     466     960 
   Size:        466     494
 
Bme1580I G_kGCm'C

Cuts at:      0     554     960 
   Size:        554     406
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     264     960 
   Size:        264     696
 
Bpu10I CC'TnA_GC

Cuts at:      0     147     220     960 
   Size:        147      73     740

  Fragments arranged by size: 

                 740     147      73
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     793     960 
   Size:        691     102     167

  Fragments arranged by size: 

                 691     167     102
 
BsaBI GATnn'nnATC

Cuts at:      0     191     960 
   Size:        191     769
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     954     960 
   Size:        954       6
 
BsmI GAATG_Cn'

Cuts at:      0     278     960 
   Size:        278     682
 
Bsp1286I G_dGCh'C

Cuts at:      0     554     960 
   Size:        554     406
 
BsrDI GCAATG_nn'

Cuts at:      0     389     797     960 
   Size:        389     408     163

  Fragments arranged by size: 

                 408     389     163
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     322     960 
   Size:        322     638
 
BstEII G'GTnAC_C

Cuts at:      0     527     855     960 
   Size:        527     328     105

  Fragments arranged by size: 

                 527     328     105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     312     960 
   Size:        312     648
 
BstZ17I GTA'TAC

Cuts at:      0     897     960 
   Size:        897      63
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     607     960 
   Size:        607     353
 
EaeI y'GGCC_r

Cuts at:      0     404     960 
   Size:        404     556
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     562     960 
   Size:        562     398
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     264     701     960 
   Size:        221      43     437     259

  Fragments arranged by size: 

                 437     259     221      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0     473     960 
   Size:        473     487
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     483     960 
   Size:        483     477
 
MscI TGG'CCA

Cuts at:      0     406     960 
   Size:        406     554
 
MslI CAynn'nnrTG

Cuts at:      0     166     205     331     382     960 
   Size:        166      39     126      51     578

  Fragments arranged by size: 

                 578     166     126      51      39
 
MspA1I CmG'CkG

Cuts at:      0     788     846     960 
   Size:        788      58     114

  Fragments arranged by size: 

                 788     114      58
 
NspI r_CATG'y

Cuts at:      0     228     960 
   Size:        228     732
 
PstI C_TGCA'G

Cuts at:      0     481     960 
   Size:        481     479
 
PvuII CAG'CTG

Cuts at:      0     788     846     960 
   Size:        788      58     114

  Fragments arranged by size: 

                 788     114      58
 
SfcI C'TryA_G

Cuts at:      0     477     783     960 
   Size:        477     306     177

  Fragments arranged by size: 

                 477     306     177
 
SmlI C'TyrA_G

Cuts at:      0     706     808     960 
   Size:        706     102     152

  Fragments arranged by size: 

                 706     152     102
 
SphI G_CATG'C

Cuts at:      0     228     960 
   Size:        228     732
 
StyI C'CwwG_G

Cuts at:      0      75     960 
   Size:         75     885
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     219     960 
   Size:        219     741
 
TatI w'GTAC_w

Cuts at:      0     209     960 
   Size:        209     751
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     BanI    BciVI Bme1580I     BpmI   Bpu10I
    BpuEI    BsaBI     BsgI     BsmI Bsp1286I    BsrDI   BstAPI   BstEII
    BstXI  BstZ17I     DrdI     EaeI     EciI  Eco57MI EcoO109I     MmeI
     MscI     MslI   MspA1I     NspI     PstI    PvuII     SfcI     SmlI
     SphI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI