(Linear) (Six-base) MAPSORT of: KIEE6065.seq  Check: 7677  from: 1  to: 960



KIEE6065.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:21  ..

AccI GT'mk_AC

Cuts at:      0     569     960 
   Size:        569     391
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     922     960 
   Size:        922      38
 
AflII C'TTAA_G

Cuts at:      0     873     960 
   Size:        873      87
 
AflIII A'CryG_T

Cuts at:      0     311     502     770     960 
   Size:        311     191     268     190

  Fragments arranged by size: 

                 311     268     191     190
 
AlwNI CAG_nnn'CTG

Cuts at:      0     243     960 
   Size:        243     717
 
ApoI r'AATT_y

Cuts at:      0     192     492     960 
   Size:        192     300     468

  Fragments arranged by size: 

                 468     300     192
 
BanII G_rGCy'C

Cuts at:      0     325     960 
   Size:        325     635
 
BglII A'GATC_T

Cuts at:      0     207     960 
   Size:        207     753
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     465     960 
   Size:        465     495
 
BsaBI GATnn'nnATC

Cuts at:      0     433     960 
   Size:        433     527
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     438     468     960 
   Size:        438      30     492

  Fragments arranged by size: 

                 492     438      30
 
BseYI C'CCAG_C

Cuts at:      0     158     960 
   Size:        158     802
 
Bsp1286I G_dGCh'C

Cuts at:      0     325     960 
   Size:        325     635
 
BspHI T'CATG_A

Cuts at:      0     404     437     960 
   Size:        404      33     523

  Fragments arranged by size: 

                 523     404      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     785     960 
   Size:        785     175
 
BsrBI CCG'CTC

Cuts at:      0     861     960 
   Size:        861      99
 
BsrDI GCAATG_nn'

Cuts at:      0     659     716     960 
   Size:        659      57     244

  Fragments arranged by size: 

                 659     244      57
 
BsrGI T'GTAC_A

Cuts at:      0     478     960 
   Size:        478     482
 
BstYI r'GATC_y

Cuts at:      0     207     724     960 
   Size:        207     517     236

  Fragments arranged by size: 

                 517     236     207
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     564     599     960 
   Size:        564      35     361

  Fragments arranged by size: 

                 564     361      35
 
EaeI y'GGCC_r

Cuts at:      0     458     960 
   Size:        458     502
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     465     922     960 
   Size:        465     457      38

  Fragments arranged by size: 

                 465     457      38
 
EcoO109I rG'GnC_Cy

Cuts at:      0     259     960 
   Size:        259     701
 
HincII GTy'rAC

Cuts at:      0     249     960 
   Size:        249     711
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     321     960 
   Size:        321     639
 
MscI TGG'CCA

Cuts at:      0     460     960 
   Size:        460     500
 
MslI CAynn'nnrTG

Cuts at:      0     417     960 
   Size:        417     543
 
NspI r_CATG'y

Cuts at:      0     315     506     774     960 
   Size:        315     191     268     186

  Fragments arranged by size: 

                 315     268     191     186
 
PciI A'CATG_T

Cuts at:      0     311     502     770     960 
   Size:        311     191     268     190

  Fragments arranged by size: 

                 311     268     191     190
 
PfoI T'CCnGG_A

Cuts at:      0     149     960 
   Size:        149     811
 
PpuMI rG'GwC_Cy

Cuts at:      0     259     960 
   Size:        259     701
 
PsiI TTA'TAA

Cuts at:      0     613     960 
   Size:        613     347
 
SexAI A'CCwGG_T

Cuts at:      0     783     960 
   Size:        783     177
 
SfcI C'TryA_G

Cuts at:      0     467     737     960 
   Size:        467     270     223

  Fragments arranged by size: 

                 467     270     223
 
SmlI C'TyrA_G

Cuts at:      0     873     960 
   Size:        873      87
 
TatI w'GTAC_w

Cuts at:      0     176     478     960 
   Size:        176     302     482

  Fragments arranged by size: 

                 482     302     176
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     617     649     960 
   Size:        617      32     311

  Fragments arranged by size: 

                 617     311      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     279     960 
   Size:        279     681
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII    AlwNI     ApoI    BanII    BglII
     BpmI    BsaBI    BsaXI    BseYI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrGI    BstYI    CspCI     EaeI  Eco57MI EcoO109I   HincII
     MmeI     MscI     MslI     NspI     PciI     PfoI    PpuMI     PsiI
    SexAI     SfcI     SmlI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI