Restriction map for
pFN21AE6063
(Linear) (Six-base) MAPSORT of: KIEE6063.seq Check: 3422 from: 1 to: 960
KIEE6063.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AleI CACnn'nnGTG
Cuts at: 0 889 960
Size: 889 71
AlwNI CAG_nnn'CTG
Cuts at: 0 246 960
Size: 246 714
ApaLI G'TGCA_C
Cuts at: 0 160 960
Size: 160 800
AvaI C'yCGr_G
Cuts at: 0 668 960
Size: 668 292
BanII G_rGCy'C
Cuts at: 0 481 641 960
Size: 481 160 319
Fragments arranged by size:
481 319 160
BciVI GTATCCnnnnn_n'
Cuts at: 0 567 960
Size: 567 393
BfrBI ATG'CAT
Cuts at: 0 411 960
Size: 411 549
BglI GCCn_nnn'nGGC
Cuts at: 0 636 960
Size: 636 324
Bme1580I G_kGCm'C
Cuts at: 0 164 960
Size: 164 796
BmtI G_CTAG'C
Cuts at: 0 436 960
Size: 436 524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 766 960
Size: 11 755 194
Fragments arranged by size:
755 194 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 960
Size: 117 843
BsaI GGTCTCn'nnnn_
Cuts at: 0 150 654 960
Size: 150 504 306
Fragments arranged by size:
504 306 150
BsaAI yAC'GTr
Cuts at: 0 735 960
Size: 735 225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 278 308 960
Size: 278 30 652
Fragments arranged by size:
652 278 30
BseYI C'CCAG_C
Cuts at: 0 321 450 960
Size: 321 129 510
Fragments arranged by size:
510 321 129
BsiHKAI G_wGCw'C
Cuts at: 0 164 799 960
Size: 164 635 161
Fragments arranged by size:
635 164 161
BsmI GAATG_Cn'
Cuts at: 0 712 960
Size: 712 248
Bsp1286I G_dGCh'C
Cuts at: 0 164 481 641 799 960
Size: 164 317 160 158 161
Fragments arranged by size:
317 164 161 160 158
BsrDI GCAATG_nn'
Cuts at: 0 132 662 960
Size: 132 530 298
Fragments arranged by size:
530 298 132
BssSI C'ACGA_G
Cuts at: 0 225 960
Size: 225 735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 474 960
Size: 471 3 486
Fragments arranged by size:
486 471 3
Bsu36I CC'TnA_GG
Cuts at: 0 877 960
Size: 877 83
ClaI AT'CG_AT
Cuts at: 0 115 960
Size: 115 845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 213 248 517 552 960
Size: 213 35 269 35 408
Fragments arranged by size:
408 269 213 35 35
EaeI y'GGCC_r
Cuts at: 0 347 605 657 960
Size: 347 258 52 303
Fragments arranged by size:
347 303 258 52
EarI CTCTTCn'nnn_
Cuts at: 0 88 889 960
Size: 88 801 71
Fragments arranged by size:
801 88 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 717 960
Size: 717 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 766 960
Size: 11 755 194
Fragments arranged by size:
755 194 11
HaeII r_GCGC'y
Cuts at: 0 902 960
Size: 902 58
MfeI C'AATT_G
Cuts at: 0 516 960
Size: 516 444
MscI TGG'CCA
Cuts at: 0 349 607 960
Size: 349 258 353
Fragments arranged by size:
353 349 258
MslI CAynn'nnrTG
Cuts at: 0 155 175 469 772 889 960
Size: 155 20 294 303 117 71
Fragments arranged by size:
303 294 155 117 71 20
MspA1I CmG'CkG
Cuts at: 0 504 960
Size: 504 456
NheI G'CTAG_C
Cuts at: 0 432 960
Size: 432 528
NsiI A_TGCA'T
Cuts at: 0 413 960
Size: 413 547
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 960
Size: 321 639
PfoI T'CCnGG_A
Cuts at: 0 206 960
Size: 206 754
PmlI CAC'GTG
Cuts at: 0 735 960
Size: 735 225
PstI C_TGCA'G
Cuts at: 0 385 942 960
Size: 385 557 18
Fragments arranged by size:
557 385 18
SapI GCTCTTCn'nnn_
Cuts at: 0 889 960
Size: 889 71
SfcI C'TryA_G
Cuts at: 0 381 938 960
Size: 381 557 22
Fragments arranged by size:
557 381 22
SmaI CCC'GGG
Cuts at: 0 670 960
Size: 670 290
SmlI C'TyrA_G
Cuts at: 0 96 960
Size: 96 864
SrfI GCCC'GGGC
Cuts at: 0 670 960
Size: 670 290
StyI C'CwwG_G
Cuts at: 0 426 960
Size: 426 534
TatI w'GTAC_w
Cuts at: 0 179 960
Size: 179 781
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 960
Size: 278 32 650
Fragments arranged by size:
650 278 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 960
Size: 471 489
XmaI C'CCGG_G
Cuts at: 0 668 960
Size: 668 292
Enzymes that do cut:
AleI AlwNI ApaLI AvaI BanII BciVI BfrBI BglI
Bme1580I BmtI BpmI BpuEI BsaI BsaAI BsaXI BseYI
BsiHKAI BsmI Bsp1286I BsrDI BssSI BstXI Bsu36I ClaI
CspCI EaeI EarI EciI Eco57MI HaeII MfeI MscI
MslI MspA1I NheI NsiI PflMI PfoI PmlI PstI
SapI SfcI SmaI SmlI SrfI StyI TatI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BclI BglII BlpI BmgBI BmrI BplI
Bpu10I BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspI PacI PasI PciI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TaqII Tth111I XbaI XhoI
XmnI ZraI