Restriction map for
pFN21AE6060
(Linear) (Six-base) MAPSORT of: KIEE6060.seq Check: 5778 from: 1 to: 960
KIEE6060.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 960
Size: 721 239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 960
Size: 554 406
AflIII A'CryG_T
Cuts at: 0 723 913 960
Size: 723 190 47
Fragments arranged by size:
723 190 47
AleI CACnn'nnGTG
Cuts at: 0 223 960
Size: 223 737
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 491 960
Size: 491 469
ApaLI G'TGCA_C
Cuts at: 0 503 960
Size: 503 457
AvrII C'CTAG_G
Cuts at: 0 36 960
Size: 36 924
BanII G_rGCy'C
Cuts at: 0 63 960
Size: 63 897
BbsI GAAGACnn'nnnn_
Cuts at: 0 567 960
Size: 567 393
BglII A'GATC_T
Cuts at: 0 293 960
Size: 293 667
Bme1580I G_kGCm'C
Cuts at: 0 230 507 960
Size: 230 277 453
Fragments arranged by size:
453 277 230
BmgBI CAC'GTC
Cuts at: 0 204 914 960
Size: 204 710 46
Fragments arranged by size:
710 204 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 322 960
Size: 153 169 638
Fragments arranged by size:
638 169 153
Bpu10I CC'TnA_GC
Cuts at: 0 181 484 960
Size: 181 303 476
Fragments arranged by size:
476 303 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 655 768 960
Size: 655 113 192
Fragments arranged by size:
655 192 113
BsaWI w'CCGG_w
Cuts at: 0 253 960
Size: 253 707
BseYI C'CCAG_C
Cuts at: 0 906 960
Size: 906 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 470 960
Size: 310 160 490
Fragments arranged by size:
490 310 160
BsiHKAI G_wGCw'C
Cuts at: 0 507 960
Size: 507 453
Bsp1286I G_dGCh'C
Cuts at: 0 63 230 507 960
Size: 63 167 277 453
Fragments arranged by size:
453 277 167 63
BspHI T'CATG_A
Cuts at: 0 77 125 398 960
Size: 77 48 273 562
Fragments arranged by size:
562 273 77 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 960
Size: 721 239
BsrDI GCAATG_nn'
Cuts at: 0 123 960
Size: 123 837
BsrFI r'CCGG_y
Cuts at: 0 152 960
Size: 152 808
BsrGI T'GTAC_A
Cuts at: 0 590 960
Size: 590 370
BssSI C'ACGA_G
Cuts at: 0 508 960
Size: 508 452
BstBI TT'CG_AA
Cuts at: 0 578 960
Size: 578 382
BstYI r'GATC_y
Cuts at: 0 293 960
Size: 293 667
Bsu36I CC'TnA_GG
Cuts at: 0 418 960
Size: 418 542
BtgI C'CryG_G
Cuts at: 0 344 960
Size: 344 616
ClaI AT'CG_AT
Cuts at: 0 515 960
Size: 515 445
DraIII CAC_nnn'GTG
Cuts at: 0 316 960
Size: 316 644
DrdI GACnn_nn'nnGTC
Cuts at: 0 432 571 960
Size: 432 139 389
Fragments arranged by size:
432 389 139
EaeI y'GGCC_r
Cuts at: 0 89 960
Size: 89 871
EarI CTCTTCn'nnn_
Cuts at: 0 79 723 960
Size: 79 644 237
Fragments arranged by size:
644 237 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 960
Size: 554 406
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 960
Size: 466 494
EcoO109I rG'GnC_Cy
Cuts at: 0 534 960
Size: 534 426
EcoRV GAT'ATC
Cuts at: 0 388 960
Size: 388 572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 720 960
Size: 720 240
MscI TGG'CCA
Cuts at: 0 91 960
Size: 91 869
MslI CAynn'nnrTG
Cuts at: 0 166 202 223 397 960
Size: 166 36 21 174 563
Fragments arranged by size:
563 174 166 36 21
MspA1I CmG'CkG
Cuts at: 0 411 454 960
Size: 411 43 506
Fragments arranged by size:
506 411 43
NcoI C'CATG_G
Cuts at: 0 344 960
Size: 344 616
NspI r_CATG'y
Cuts at: 0 727 960
Size: 727 233
PciI A'CATG_T
Cuts at: 0 723 960
Size: 723 237
PstI C_TGCA'G
Cuts at: 0 470 960
Size: 470 490
PvuII CAG'CTG
Cuts at: 0 411 454 960
Size: 411 43 506
Fragments arranged by size:
506 411 43
SapI GCTCTTCn'nnn_
Cuts at: 0 723 960
Size: 723 237
SbfI CC_TGCA'GG
Cuts at: 0 470 960
Size: 470 490
SfcI C'TryA_G
Cuts at: 0 466 683 861 960
Size: 466 217 178 99
Fragments arranged by size:
466 217 178 99
StuI AGG'CCT
Cuts at: 0 703 960
Size: 703 257
StyI C'CwwG_G
Cuts at: 0 36 344 960
Size: 36 308 616
Fragments arranged by size:
616 308 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 304 392 436 960
Size: 304 88 44 524
Fragments arranged by size:
524 304 88 44
TatI w'GTAC_w
Cuts at: 0 170 590 960
Size: 170 420 370
Fragments arranged by size:
420 370 170
Tth111I GACn'n_nGTC
Cuts at: 0 429 960
Size: 429 531
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI ApaLI AvrII BanII
BbsI BglII Bme1580I BmgBI BmrI Bpu10I BsaI BsaWI
BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstBI BstYI Bsu36I BtgI ClaI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI
MscI MslI MspA1I NcoI NspI PciI PstI PvuII
SapI SbfI SfcI StuI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AlwNI ApaI ApoI AscI AseI AsiSI AvaI
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmtI BplI BpmI BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BssHII BstAPI BstEII BstXI BstZ17I BtgZI
BtsI CspCI DraI EagI EciI EcoICRI EcoRI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI XbaI XcmI
XhoI XmaI XmnI ZraI