Restriction map for
pFN21AE6059
(Linear) (Six-base) MAPSORT of: KIEE6059.seq Check: 9882 from: 1 to: 960
KIEE6059.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 733 960
Size: 733 227
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 960
Size: 702 258
AflII C'TTAA_G
Cuts at: 0 931 960
Size: 931 29
AflIII A'CryG_T
Cuts at: 0 548 960
Size: 548 412
AhdI GACnn_n'nnGTC
Cuts at: 0 549 960
Size: 549 411
AleI CACnn'nnGTG
Cuts at: 0 214 740 960
Size: 214 526 220
Fragments arranged by size:
526 220 214
AlwNI CAG_nnn'CTG
Cuts at: 0 725 960
Size: 725 235
ApaLI G'TGCA_C
Cuts at: 0 225 316 960
Size: 225 91 644
Fragments arranged by size:
644 225 91
BanI G'GyrC_C
Cuts at: 0 16 960
Size: 16 944
BbsI GAAGACnn'nnnn_
Cuts at: 0 180 960
Size: 180 780
Bme1580I G_kGCm'C
Cuts at: 0 229 320 960
Size: 229 91 640
Fragments arranged by size:
640 229 91
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 960
Size: 14 946
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 960
Size: 256 704
BseYI C'CCAG_C
Cuts at: 0 436 960
Size: 436 524
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 711 960
Size: 711 249
BsiHKAI G_wGCw'C
Cuts at: 0 221 229 320 350 960
Size: 221 8 91 30 610
Fragments arranged by size:
610 221 91 30 8
Bsp1286I G_dGCh'C
Cuts at: 0 221 229 320 350 960
Size: 221 8 91 30 610
Fragments arranged by size:
610 221 91 30 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 891 960
Size: 733 158 69
Fragments arranged by size:
733 158 69
BsrDI GCAATG_nn'
Cuts at: 0 113 425 960
Size: 113 312 535
Fragments arranged by size:
535 312 113
BsrGI T'GTAC_A
Cuts at: 0 107 960
Size: 107 853
BstYI r'GATC_y
Cuts at: 0 83 960
Size: 83 877
BtsI GCAGTG_nn'
Cuts at: 0 728 960
Size: 728 232
DraI TTT'AAA
Cuts at: 0 288 960
Size: 288 672
DraIII CAC_nnn'GTG
Cuts at: 0 375 497 960
Size: 375 122 463
Fragments arranged by size:
463 375 122
EaeI y'GGCC_r
Cuts at: 0 377 557 960
Size: 377 180 403
Fragments arranged by size:
403 377 180
EarI CTCTTCn'nnn_
Cuts at: 0 256 960
Size: 256 704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 702 960
Size: 14 688 258
Fragments arranged by size:
688 258 14
EcoRV GAT'ATC
Cuts at: 0 566 636 960
Size: 566 70 324
Fragments arranged by size:
566 324 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 899 931 960
Size: 535 32 332 32 29
Fragments arranged by size:
535 332 32 32 29
HincII GTy'rAC
Cuts at: 0 588 960
Size: 588 372
HpaI GTT'AAC
Cuts at: 0 588 960
Size: 588 372
MscI TGG'CCA
Cuts at: 0 379 559 960
Size: 379 180 401
Fragments arranged by size:
401 379 180
MslI CAynn'nnrTG
Cuts at: 0 214 331 355 553 740 960
Size: 214 117 24 198 187 220
Fragments arranged by size:
220 214 198 187 117 24
NspI r_CATG'y
Cuts at: 0 552 960
Size: 552 408
PciI A'CATG_T
Cuts at: 0 548 960
Size: 548 412
PflMI CCAn_nnn'nTGG
Cuts at: 0 375 960
Size: 375 585
SmlI C'TyrA_G
Cuts at: 0 271 931 960
Size: 271 660 29
Fragments arranged by size:
660 271 29
StuI AGG'CCT
Cuts at: 0 166 715 960
Size: 166 549 245
Fragments arranged by size:
549 245 166
TatI w'GTAC_w
Cuts at: 0 107 960
Size: 107 853
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 747 779 960
Size: 747 32 181
Fragments arranged by size:
747 181 32
XmnI GAAnn'nnTTC
Cuts at: 0 715 960
Size: 715 245
Enzymes that do cut:
AarI AcuI AflII AflIII AhdI AleI AlwNI ApaLI
BanI BbsI Bme1580I BpmI BpuEI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrGI BstYI BtsI DraI DraIII
EaeI EarI Eco57MI EcoRV Hin4I HincII HpaI MscI
MslI NspI PciI PflMI SmlI StuI TatI TstI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AlfI AloI
ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI