Restriction map for
pFN21AE6058
(Linear) (Six-base) MAPSORT of: KIEE6058.seq Check: 9672 from: 1 to: 960
KIEE6058.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 879 960
Size: 879 81
AflIII A'CryG_T
Cuts at: 0 733 960
Size: 733 227
AleI CACnn'nnGTG
Cuts at: 0 749 960
Size: 749 211
ApaI G_GGCC'C
Cuts at: 0 113 281 900 960
Size: 113 168 619 60
Fragments arranged by size:
619 168 113 60
ApaLI G'TGCA_C
Cuts at: 0 551 832 960
Size: 551 281 128
Fragments arranged by size:
551 281 128
AvaI C'yCGr_G
Cuts at: 0 704 960
Size: 704 256
BanII G_rGCy'C
Cuts at: 0 113 281 900 960
Size: 113 168 619 60
Fragments arranged by size:
619 168 113 60
BglI GCCn_nnn'nGGC
Cuts at: 0 211 960
Size: 211 749
BlpI GC'TnA_GC
Cuts at: 0 231 960
Size: 231 729
Bme1580I G_kGCm'C
Cuts at: 0 84 113 281 555 836 900 960
Size: 84 29 168 274 281 64 60
Fragments arranged by size:
281 274 168 84 64 60 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 940 960
Size: 940 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 784 911 960
Size: 784 127 49
Fragments arranged by size:
784 127 49
BsaHI Gr'CG_yC
Cuts at: 0 396 960
Size: 396 564
BseYI C'CCAG_C
Cuts at: 0 132 960
Size: 132 828
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 388 960
Size: 163 225 572
Fragments arranged by size:
572 225 163
BsiHKAI G_wGCw'C
Cuts at: 0 147 518 555 836 960
Size: 147 371 37 281 124
Fragments arranged by size:
371 281 147 124 37
Bsp1286I G_dGCh'C
Cuts at: 0 84 113 147 281 518 555 836 900
Size: 84 29 34 134 237 37 281 64
Cuts at: 900 960
Size: 60
Fragments arranged by size:
281 237 134 84 64 60 37 34
29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 879 960
Size: 879 81
BsrDI GCAATG_nn'
Cuts at: 0 144 674 960
Size: 144 530 286
Fragments arranged by size:
530 286 144
BsrFI r'CCGG_y
Cuts at: 0 150 960
Size: 150 810
BsrGI T'GTAC_A
Cuts at: 0 324 960
Size: 324 636
BstAPI GCAn_nnn'nTGC
Cuts at: 0 337 960
Size: 337 623
BstEII G'GTnAC_C
Cuts at: 0 420 960
Size: 420 540
BstXI CCAn_nnnn'nTGG
Cuts at: 0 225 960
Size: 225 735
BtgI C'CryG_G
Cuts at: 0 341 365 960
Size: 341 24 595
Fragments arranged by size:
595 341 24
BtsI GCAGTG_nn'
Cuts at: 0 145 161 175 400 493 569 960
Size: 145 16 14 225 93 76 391
Fragments arranged by size:
391 225 145 93 76 16 14
EaeI y'GGCC_r
Cuts at: 0 203 362 626 960
Size: 203 159 264 334
Fragments arranged by size:
334 264 203 159
EarI CTCTTCn'nnn_
Cuts at: 0 787 960
Size: 787 173
EcoO109I rG'GnC_Cy
Cuts at: 0 278 896 960
Size: 278 618 64
Fragments arranged by size:
618 278 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 960
Size: 872 88
MscI TGG'CCA
Cuts at: 0 205 364 628 960
Size: 205 159 264 332
Fragments arranged by size:
332 264 205 159
MslI CAynn'nnrTG
Cuts at: 0 562 749 960
Size: 562 187 211
Fragments arranged by size:
562 211 187
NaeI GCC'GGC
Cuts at: 0 152 960
Size: 152 808
NcoI C'CATG_G
Cuts at: 0 341 365 960
Size: 341 24 595
Fragments arranged by size:
595 341 24
NdeI CA'TA_TG
Cuts at: 0 294 960
Size: 294 666
NgoMIV G'CCGG_C
Cuts at: 0 150 960
Size: 150 810
NspI r_CATG'y
Cuts at: 0 331 737 960
Size: 331 406 223
Fragments arranged by size:
406 331 223
PasI CC'CwG_GG
Cuts at: 0 250 960
Size: 250 710
PciI A'CATG_T
Cuts at: 0 733 960
Size: 733 227
PfoI T'CCnGG_A
Cuts at: 0 926 960
Size: 926 34
PshAI GACnn'nnGTC
Cuts at: 0 100 960
Size: 100 860
PspOMI G'GGCC_C
Cuts at: 0 109 277 896 960
Size: 109 168 619 64
Fragments arranged by size:
619 168 109 64
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 723 755 960
Size: 723 32 205
Fragments arranged by size:
723 205 32
PstI C_TGCA'G
Cuts at: 0 695 960
Size: 695 265
SexAI A'CCwGG_T
Cuts at: 0 774 960
Size: 774 186
SfcI C'TryA_G
Cuts at: 0 242 691 960
Size: 242 449 269
Fragments arranged by size:
449 269 242
SmaI CCC'GGG
Cuts at: 0 706 960
Size: 706 254
StuI AGG'CCT
Cuts at: 0 724 763 960
Size: 724 39 197
Fragments arranged by size:
724 197 39
StyI C'CwwG_G
Cuts at: 0 341 365 449 719 960
Size: 341 24 84 270 241
Fragments arranged by size:
341 270 241 84 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 53 935 960
Size: 53 882 25
Fragments arranged by size:
882 53 25
TatI w'GTAC_w
Cuts at: 0 324 960
Size: 324 636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 120 432 636 960
Size: 120 312 204 324
Fragments arranged by size:
324 312 204 120
XmaI C'CCGG_G
Cuts at: 0 704 960
Size: 704 256
Enzymes that do cut:
AarI AflIII AleI ApaI ApaLI AvaI BanII BglI
BlpI Bme1580I BplI BsaI BsaHI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI
BtgI BtsI EaeI EarI EcoO109I MmeI MscI MslI
NaeI NcoI NdeI NgoMIV NspI PasI PciI PfoI
PshAI PspOMI PsrI PstI SexAI SfcI SmaI StuI
StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI
BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MspA1I NarI
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpiI PpuMI PsiI PspXI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmnI ZraI