Restriction map for
pFN21AE6055
(Linear) (Six-base) MAPSORT of: KIEE6055.seq Check: 6529 from: 1 to: 957
KIEE6055.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AccI GT'mk_AC
Cuts at: 0 377 957
Size: 377 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 957
Size: 391 566
AflIII A'CryG_T
Cuts at: 0 947 957
Size: 947 10
AlwNI CAG_nnn'CTG
Cuts at: 0 927 957
Size: 927 30
ApaLI G'TGCA_C
Cuts at: 0 887 957
Size: 887 70
ApoI r'AATT_y
Cuts at: 0 604 957
Size: 604 353
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 178 211 759 792 957
Size: 178 33 548 33 165
Fragments arranged by size:
548 178 165 33 33
BanI G'GyrC_C
Cuts at: 0 136 957
Size: 136 821
BbsI GAAGACnn'nnnn_
Cuts at: 0 432 957
Size: 432 525
BbvCI CC'TCA_GC
Cuts at: 0 694 957
Size: 694 263
BglII A'GATC_T
Cuts at: 0 317 957
Size: 317 640
Bme1580I G_kGCm'C
Cuts at: 0 891 957
Size: 891 66
BmgBI CAC'GTC
Cuts at: 0 948 957
Size: 948 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 42 484 922 946 957
Size: 42 442 438 24 11
Fragments arranged by size:
442 438 42 24 11
BmtI G_CTAG'C
Cuts at: 0 625 957
Size: 625 332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 22 957
Size: 15 7 935
Fragments arranged by size:
935 15 7
Bpu10I CC'TnA_GC
Cuts at: 0 466 694 957
Size: 466 228 263
Fragments arranged by size:
466 263 228
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 957
Size: 166 791
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 957
Size: 82 30 845
Fragments arranged by size:
845 82 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 957
Size: 136 821
BseYI C'CCAG_C
Cuts at: 0 204 957
Size: 204 753
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 851 957
Size: 832 19 106
Fragments arranged by size:
832 106 19
BsiHKAI G_wGCw'C
Cuts at: 0 545 736 891 957
Size: 545 191 155 66
Fragments arranged by size:
545 191 155 66
BsmI GAATG_Cn'
Cuts at: 0 74 957
Size: 74 883
Bsp1286I G_dGCh'C
Cuts at: 0 545 736 891 957
Size: 545 191 155 66
Fragments arranged by size:
545 191 155 66
BspHI T'CATG_A
Cuts at: 0 78 957
Size: 78 879
BsrDI GCAATG_nn'
Cuts at: 0 722 957
Size: 722 235
BstXI CCAn_nnnn'nTGG
Cuts at: 0 261 957
Size: 261 696
BstYI r'GATC_y
Cuts at: 0 257 317 957
Size: 257 60 640
Fragments arranged by size:
640 257 60
BtsI GCAGTG_nn'
Cuts at: 0 224 330 543 957
Size: 224 106 213 414
Fragments arranged by size:
414 224 213 106
DraIII CAC_nnn'GTG
Cuts at: 0 690 957
Size: 690 267
EaeI y'GGCC_r
Cuts at: 0 236 263 957
Size: 236 27 694
Fragments arranged by size:
694 236 27
EarI CTCTTCn'nnn_
Cuts at: 0 410 769 957
Size: 410 359 188
Fragments arranged by size:
410 359 188
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 22 391 957
Size: 15 7 369 566
Fragments arranged by size:
566 369 15 7
HincII GTy'rAC
Cuts at: 0 482 957
Size: 482 475
HindIII A'AGCT_T
Cuts at: 0 431 957
Size: 431 526
MscI TGG'CCA
Cuts at: 0 238 265 957
Size: 238 27 692
Fragments arranged by size:
692 238 27
MslI CAynn'nnrTG
Cuts at: 0 58 247 490 595 957
Size: 58 189 243 105 362
Fragments arranged by size:
362 243 189 105 58
NheI G'CTAG_C
Cuts at: 0 621 957
Size: 621 336
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 272 957
Size: 204 68 685
Fragments arranged by size:
685 204 68
PfoI T'CCnGG_A
Cuts at: 0 526 957
Size: 526 431
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 592 624 957
Size: 592 32 333
Fragments arranged by size:
592 333 32
StyI C'CwwG_G
Cuts at: 0 284 722 957
Size: 284 438 235
Fragments arranged by size:
438 284 235
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 508 540 957
Size: 82 32 394 32 417
Fragments arranged by size:
417 394 82 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 938 957
Size: 938 19
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaLI ApoI BaeI BanI
BbsI BbvCI BglII Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BstXI BstYI BtsI DraIII EaeI
EarI Eco57MI HincII HindIII MscI MslI NheI PflMI
PfoI PsrI StyI TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BamHI BanII BbeI BcgI BciVI BclI BfrBI
BglI BlpI BplI BpuEI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI XbaI XcmI XhoI XmaI XmnI ZraI