Restriction map for
pFN21AE6052
(Linear) (Six-base) MAPSORT of: KIEE6052.seq Check: 5952 from: 1 to: 954
KIEE6052.orf
With 182 enzymes: SgfI *
December 5, 2014 13:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 954
Size: 929 25
AflII C'TTAA_G
Cuts at: 0 673 954
Size: 673 281
AflIII A'CryG_T
Cuts at: 0 694 954
Size: 694 260
BamHI G'GATC_C
Cuts at: 0 9 954
Size: 9 945
BanI G'GyrC_C
Cuts at: 0 477 954
Size: 477 477
BanII G_rGCy'C
Cuts at: 0 182 953 954
Size: 182 771 1
Fragments arranged by size:
771 182 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 483 895 954
Size: 483 412 59
Fragments arranged by size:
483 412 59
BsaAI yAC'GTr
Cuts at: 0 697 954
Size: 697 257
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 954
Size: 452 502
BsiHKAI G_wGCw'C
Cuts at: 0 106 167 866 954
Size: 106 61 699 88
Fragments arranged by size:
699 106 88 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 735 954
Size: 735 219
Bsp1286I G_dGCh'C
Cuts at: 0 106 167 182 866 953 954
Size: 106 61 15 684 87 1
Fragments arranged by size:
684 106 87 61 15 1
BspHI T'CATG_A
Cuts at: 0 560 954
Size: 560 394
BsrBI CCG'CTC
Cuts at: 0 518 954
Size: 518 436
BsrDI GCAATG_nn'
Cuts at: 0 27 954
Size: 27 927
BssSI C'ACGA_G
Cuts at: 0 425 954
Size: 425 529
BstAPI GCAn_nnn'nTGC
Cuts at: 0 171 954
Size: 171 783
BstXI CCAn_nnnn'nTGG
Cuts at: 0 252 633 954
Size: 252 381 321
Fragments arranged by size:
381 321 252
BstYI r'GATC_y
Cuts at: 0 9 954
Size: 9 945
BtgI C'CryG_G
Cuts at: 0 359 782 954
Size: 359 423 172
Fragments arranged by size:
423 359 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 775 954
Size: 775 179
BtsI GCAGTG_nn'
Cuts at: 0 394 954
Size: 394 560
EaeI y'GGCC_r
Cuts at: 0 254 356 380 833 932 954
Size: 254 102 24 453 99 22
Fragments arranged by size:
453 254 102 99 24 22
EarI CTCTTCn'nnn_
Cuts at: 0 65 954
Size: 65 889
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 612 954
Size: 612 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 954
Size: 929 25
EcoRV GAT'ATC
Cuts at: 0 582 954
Size: 582 372
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 896 928 954
Size: 26 870 32 26
Fragments arranged by size:
870 32 26 26
MfeI C'AATT_G
Cuts at: 0 872 954
Size: 872 82
MscI TGG'CCA
Cuts at: 0 256 358 382 835 934 954
Size: 256 102 24 453 99 20
Fragments arranged by size:
453 256 102 99 24 20
MslI CAynn'nnrTG
Cuts at: 0 554 740 954
Size: 554 186 214
Fragments arranged by size:
554 214 186
MspA1I CmG'CkG
Cuts at: 0 504 819 954
Size: 504 315 135
Fragments arranged by size:
504 315 135
NcoI C'CATG_G
Cuts at: 0 359 782 954
Size: 359 423 172
Fragments arranged by size:
423 359 172
NspI r_CATG'y
Cuts at: 0 204 954
Size: 204 750
PflMI CCAn_nnn'nTGG
Cuts at: 0 263 954
Size: 263 691
PmlI CAC'GTG
Cuts at: 0 697 954
Size: 697 257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 954
Size: 64 32 858
Fragments arranged by size:
858 64 32
PvuII CAG'CTG
Cuts at: 0 504 954
Size: 504 450
ScaI AGT'ACT
Cuts at: 0 413 954
Size: 413 541
SfcI C'TryA_G
Cuts at: 0 304 954
Size: 304 650
SmlI C'TyrA_G
Cuts at: 0 673 954
Size: 673 281
SphI G_CATG'C
Cuts at: 0 204 954
Size: 204 750
StuI AGG'CCT
Cuts at: 0 56 954
Size: 56 898
StyI C'CwwG_G
Cuts at: 0 359 710 782 954
Size: 359 351 72 172
Fragments arranged by size:
359 351 172 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 407 954
Size: 407 547
TatI w'GTAC_w
Cuts at: 0 411 954
Size: 411 543
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 328 351 441 954
Size: 328 23 90 513
Fragments arranged by size:
513 328 90 23
Enzymes that do cut:
AcuI AflII AflIII BamHI BanI BanII BbsI BsaAI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BssSI
BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI
EciI Eco57MI EcoRV Hin4I MfeI MscI MslI MspA1I
NcoI NspI PflMI PmlI PpiI PvuII ScaI SfcI
SmlI SphI StuI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI
BspEI BspMI BsrFI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI