Restriction map for
pFN21AE6046
(Linear) (Six-base) MAPSORT of: KIEE6046.seq Check: 3520 from: 1 to: 954
KIEE6046.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 170 954
Size: 170 784
AccI GT'mk_AC
Cuts at: 0 712 954
Size: 712 242
AlwNI CAG_nnn'CTG
Cuts at: 0 387 954
Size: 387 567
ApaLI G'TGCA_C
Cuts at: 0 422 954
Size: 422 532
AvrII C'CTAG_G
Cuts at: 0 235 954
Size: 235 719
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 563 596 954
Size: 563 33 358
Fragments arranged by size:
563 358 33
BanII G_rGCy'C
Cuts at: 0 50 954
Size: 50 904
BbvCI CC'TCA_GC
Cuts at: 0 545 954
Size: 545 409
BclI T'GATC_A
Cuts at: 0 551 881 954
Size: 551 330 73
Fragments arranged by size:
551 330 73
BglII A'GATC_T
Cuts at: 0 332 954
Size: 332 622
Bme1580I G_kGCm'C
Cuts at: 0 426 954
Size: 426 528
BmrI ACTGGGnnnn_n'
Cuts at: 0 52 954
Size: 52 902
Bpu10I CC'TnA_GC
Cuts at: 0 545 954
Size: 545 409
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 954
Size: 55 30 869
Fragments arranged by size:
869 55 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 921 954
Size: 921 33
BseYI C'CCAG_C
Cuts at: 0 380 954
Size: 380 574
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 373 549 954
Size: 148 225 176 405
Fragments arranged by size:
405 225 176 148
BsiHKAI G_wGCw'C
Cuts at: 0 426 941 954
Size: 426 515 13
Fragments arranged by size:
515 426 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 816 954
Size: 816 138
Bsp1286I G_dGCh'C
Cuts at: 0 50 426 941 954
Size: 50 376 515 13
Fragments arranged by size:
515 376 50 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 954
Size: 170 784
BsrDI GCAATG_nn'
Cuts at: 0 368 728 954
Size: 368 360 226
Fragments arranged by size:
368 360 226
BstEII G'GTnAC_C
Cuts at: 0 615 738 954
Size: 615 123 216
Fragments arranged by size:
615 216 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 319 954
Size: 319 635
BstYI r'GATC_y
Cuts at: 0 332 954
Size: 332 622
BtgI C'CryG_G
Cuts at: 0 797 954
Size: 797 157
BtsI GCAGTG_nn'
Cuts at: 0 658 954
Size: 658 296
DrdI GACnn_nn'nnGTC
Cuts at: 0 808 954
Size: 808 146
EaeI y'GGCC_r
Cuts at: 0 887 954
Size: 887 67
EarI CTCTTCn'nnn_
Cuts at: 0 225 307 954
Size: 225 82 647
Fragments arranged by size:
647 225 82
EcoO109I rG'GnC_Cy
Cuts at: 0 705 918 954
Size: 705 213 36
Fragments arranged by size:
705 213 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 954
Size: 241 32 681
Fragments arranged by size:
681 241 32
HincII GTy'rAC
Cuts at: 0 249 954
Size: 249 705
MfeI C'AATT_G
Cuts at: 0 827 954
Size: 827 127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 954
Size: 176 778
MscI TGG'CCA
Cuts at: 0 889 954
Size: 889 65
MslI CAynn'nnrTG
Cuts at: 0 172 733 838 889 954
Size: 172 561 105 51 65
Fragments arranged by size:
561 172 105 65 51
MspA1I CmG'CkG
Cuts at: 0 495 549 954
Size: 495 54 405
Fragments arranged by size:
495 405 54
NcoI C'CATG_G
Cuts at: 0 797 954
Size: 797 157
PflMI CCAn_nnn'nTGG
Cuts at: 0 505 954
Size: 505 449
PfoI T'CCnGG_A
Cuts at: 0 203 954
Size: 203 751
PpuMI rG'GwC_Cy
Cuts at: 0 918 954
Size: 918 36
PvuII CAG'CTG
Cuts at: 0 495 549 954
Size: 495 54 405
Fragments arranged by size:
495 405 54
SfcI C'TryA_G
Cuts at: 0 113 954
Size: 113 841
StuI AGG'CCT
Cuts at: 0 780 954
Size: 780 174
StyI C'CwwG_G
Cuts at: 0 235 797 954
Size: 235 562 157
Fragments arranged by size:
562 235 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 435 954
Size: 435 519
TatI w'GTAC_w
Cuts at: 0 471 954
Size: 471 483
Enzymes that do cut:
AarI AccI AlwNI ApaLI AvrII BaeI BanII BbvCI
BclI BglII Bme1580I BmrI Bpu10I BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BstEII BstXI
BstYI BtgI BtsI DrdI EaeI EarI EcoO109I Hin4I
HincII MfeI MmeI MscI MslI MspA1I NcoI PflMI
PfoI PpuMI PvuII SfcI StuI StyI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvaI BamHI BanI BbeI BbsI BcgI BciVI
BfrBI BglI BlpI BmgBI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI