Restriction map for
pFN21AE6045
(Linear) (Six-base) MAPSORT of: KIEE6045.seq Check: 8765 from: 1 to: 951
KIEE6045.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AccI GT'mk_AC
Cuts at: 0 16 238 951
Size: 16 222 713
Fragments arranged by size:
713 222 16
AflIII A'CryG_T
Cuts at: 0 317 951
Size: 317 634
ApaLI G'TGCA_C
Cuts at: 0 85 951
Size: 85 866
BamHI G'GATC_C
Cuts at: 0 674 951
Size: 674 277
BbsI GAAGACnn'nnnn_
Cuts at: 0 681 951
Size: 681 270
BbvCI CC'TCA_GC
Cuts at: 0 925 951
Size: 925 26
BciVI GTATCCnnnnn_n'
Cuts at: 0 76 951
Size: 76 875
BglI GCCn_nnn'nGGC
Cuts at: 0 577 951
Size: 577 374
Bme1580I G_kGCm'C
Cuts at: 0 89 951
Size: 89 862
BmrI ACTGGGnnnn_n'
Cuts at: 0 27 951
Size: 27 924
Bpu10I CC'TnA_GC
Cuts at: 0 925 951
Size: 925 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 951
Size: 922 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 483 951
Size: 483 468
BseYI C'CCAG_C
Cuts at: 0 60 951
Size: 60 891
BsiHKAI G_wGCw'C
Cuts at: 0 89 560 951
Size: 89 471 391
Fragments arranged by size:
471 391 89
Bsp1286I G_dGCh'C
Cuts at: 0 89 560 951
Size: 89 471 391
Fragments arranged by size:
471 391 89
BsrDI GCAATG_nn'
Cuts at: 0 386 446 455 566 951
Size: 386 60 9 111 385
Fragments arranged by size:
386 385 111 60 9
BsrGI T'GTAC_A
Cuts at: 0 476 951
Size: 476 475
BstAPI GCAn_nnn'nTGC
Cuts at: 0 457 951
Size: 457 494
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 261 951
Size: 228 33 690
Fragments arranged by size:
690 228 33
BstYI r'GATC_y
Cuts at: 0 674 951
Size: 674 277
Bsu36I CC'TnA_GG
Cuts at: 0 466 951
Size: 466 485
BtgI C'CryG_G
Cuts at: 0 368 951
Size: 368 583
BtsI GCAGTG_nn'
Cuts at: 0 143 338 951
Size: 143 195 613
Fragments arranged by size:
613 195 143
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 717 752 951
Size: 717 35 199
Fragments arranged by size:
717 199 35
EaeI y'GGCC_r
Cuts at: 0 206 365 807 951
Size: 206 159 442 144
Fragments arranged by size:
442 206 159 144
EarI CTCTTCn'nnn_
Cuts at: 0 410 951
Size: 410 541
EcoNI CCTnn'n_nnAGG
Cuts at: 0 669 951
Size: 669 282
HincII GTy'rAC
Cuts at: 0 600 951
Size: 600 351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 951
Size: 194 757
MscI TGG'CCA
Cuts at: 0 208 367 809 951
Size: 208 159 442 142
Fragments arranged by size:
442 208 159 142
MslI CAynn'nnrTG
Cuts at: 0 343 749 951
Size: 343 406 202
Fragments arranged by size:
406 343 202
NcoI C'CATG_G
Cuts at: 0 368 951
Size: 368 583
NspI r_CATG'y
Cuts at: 0 321 951
Size: 321 630
PasI CC'CwG_GG
Cuts at: 0 70 951
Size: 70 881
PciI A'CATG_T
Cuts at: 0 317 951
Size: 317 634
SfcI C'TryA_G
Cuts at: 0 239 951
Size: 239 712
SmlI C'TyrA_G
Cuts at: 0 937 951
Size: 937 14
StyI C'CwwG_G
Cuts at: 0 368 615 951
Size: 368 247 336
Fragments arranged by size:
368 336 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 951
Size: 46 905
TatI w'GTAC_w
Cuts at: 0 194 476 951
Size: 194 282 475
Fragments arranged by size:
475 282 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 852 951
Size: 852 99
Enzymes that do cut:
AccI AflIII ApaLI BamHI BbsI BbvCI BciVI BglI
Bme1580I BmrI Bpu10I BpuEI BseRI BseYI BsiHKAI Bsp1286I
BsrDI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI
CspCI EaeI EarI EcoNI HincII MmeI MscI MslI
NcoI NspI PasI PciI SfcI SmlI StyI TaqII
TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI
BcgI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI