Restriction map for
pFN21AE6043
(Linear) (Six-base) MAPSORT of: KIEE6043.seq Check: 2237 from: 1 to: 951
KIEE6043.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 951
Size: 790 161
AflIII A'CryG_T
Cuts at: 0 716 718 951
Size: 716 2 233
Fragments arranged by size:
716 233 2
AleI CACnn'nnGTG
Cuts at: 0 719 951
Size: 719 232
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 802 834 951
Size: 802 32 117
Fragments arranged by size:
802 117 32
AlwNI CAG_nnn'CTG
Cuts at: 0 59 243 951
Size: 59 184 708
Fragments arranged by size:
708 184 59
ApoI r'AATT_y
Cuts at: 0 31 919 951
Size: 31 888 32
Fragments arranged by size:
888 32 31
AvaI C'yCGr_G
Cuts at: 0 68 951
Size: 68 883
BbvCI CC'TCA_GC
Cuts at: 0 236 951
Size: 236 715
BclI T'GATC_A
Cuts at: 0 143 522 951
Size: 143 379 429
Fragments arranged by size:
429 379 143
BfrBI ATG'CAT
Cuts at: 0 408 951
Size: 408 543
BmrI ACTGGGnnnn_n'
Cuts at: 0 69 291 951
Size: 69 222 660
Fragments arranged by size:
660 222 69
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 951
Size: 774 177
Bpu10I CC'TnA_GC
Cuts at: 0 236 951
Size: 236 715
BsaI GGTCTCn'nnnn_
Cuts at: 0 78 951
Size: 78 873
BsaAI yAC'GTr
Cuts at: 0 719 951
Size: 719 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 562 592 802 832 951
Size: 562 30 210 30 119
Fragments arranged by size:
562 210 119 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 567 951
Size: 31 536 384
Fragments arranged by size:
536 384 31
BseYI C'CCAG_C
Cuts at: 0 117 951
Size: 117 834
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 493 525 951
Size: 311 182 32 426
Fragments arranged by size:
426 311 182 32
BstEII G'GTnAC_C
Cuts at: 0 108 588 951
Size: 108 480 363
Fragments arranged by size:
480 363 108
BstYI r'GATC_y
Cuts at: 0 680 951
Size: 680 271
Bsu36I CC'TnA_GG
Cuts at: 0 874 951
Size: 874 77
DrdI GACnn_nn'nnGTC
Cuts at: 0 70 951
Size: 70 881
EaeI y'GGCC_r
Cuts at: 0 398 431 761 951
Size: 398 33 330 190
Fragments arranged by size:
398 330 190 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 790 951
Size: 774 16 161
Fragments arranged by size:
774 161 16
EcoO109I rG'GnC_Cy
Cuts at: 0 896 951
Size: 896 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 562 594 951
Size: 514 32 16 32 357
Fragments arranged by size:
514 357 32 32 16
HincII GTy'rAC
Cuts at: 0 932 951
Size: 932 19
HindIII A'AGCT_T
Cuts at: 0 707 951
Size: 707 244
HpaI GTT'AAC
Cuts at: 0 932 951
Size: 932 19
MscI TGG'CCA
Cuts at: 0 433 763 951
Size: 433 330 188
Fragments arranged by size:
433 330 188
MslI CAynn'nnrTG
Cuts at: 0 103 172 595 598 719 951
Size: 103 69 423 3 121 232
Fragments arranged by size:
423 232 121 103 69 3
MspA1I CmG'CkG
Cuts at: 0 240 495 951
Size: 240 255 456
Fragments arranged by size:
456 255 240
NsiI A_TGCA'T
Cuts at: 0 410 951
Size: 410 541
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 951
Size: 59 892
PmlI CAC'GTG
Cuts at: 0 719 951
Size: 719 232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 802 834 951
Size: 802 32 117
Fragments arranged by size:
802 117 32
PvuII CAG'CTG
Cuts at: 0 240 495 951
Size: 240 255 456
Fragments arranged by size:
456 255 240
StyI C'CwwG_G
Cuts at: 0 317 951
Size: 317 634
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 12 310 951
Size: 12 298 641
Fragments arranged by size:
641 298 12
XmnI GAAnn'nnTTC
Cuts at: 0 35 709 817 951
Size: 35 674 108 134
Fragments arranged by size:
674 134 108 35
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApoI AvaI BbvCI
BclI BfrBI BmrI BpmI Bpu10I BsaI BsaAI BsaXI
BseRI BseYI BsgI BstEII BstYI Bsu36I DrdI EaeI
Eco57MI EcoO109I Hin4I HincII HindIII HpaI MscI MslI
MspA1I NsiI PflMI PmlI PpiI PvuII StyI TaqII
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PfoI PmeI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI