Restriction map for
pFN21AE6040
(Linear) (Six-base) MAPSORT of: KIEE6040.seq Check: 1010 from: 1 to: 951
KIEE6040.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 571 951
Size: 571 380
AccI GT'mk_AC
Cuts at: 0 593 951
Size: 593 358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 885 951
Size: 444 441 66
Fragments arranged by size:
444 441 66
ApoI r'AATT_y
Cuts at: 0 6 951
Size: 6 945
AvaI C'yCGr_G
Cuts at: 0 785 951
Size: 785 166
AvrII C'CTAG_G
Cuts at: 0 934 951
Size: 934 17
BamHI G'GATC_C
Cuts at: 0 680 951
Size: 680 271
BbsI GAAGACnn'nnnn_
Cuts at: 0 129 913 951
Size: 129 784 38
Fragments arranged by size:
784 129 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 226 951
Size: 52 174 725
Fragments arranged by size:
725 174 52
Bpu10I CC'TnA_GC
Cuts at: 0 318 951
Size: 318 633
BsaAI yAC'GTr
Cuts at: 0 282 951
Size: 282 669
BsaHI Gr'CG_yC
Cuts at: 0 699 951
Size: 699 252
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 8 38 45 75 199 229 742 772
Size: 8 30 7 30 124 30 513 30
Cuts at: 772 951
Size: 179
Fragments arranged by size:
513 179 124 30 30 30 30 8
7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 67 747 951
Size: 43 24 680 204
Fragments arranged by size:
680 204 43 24
BseYI C'CCAG_C
Cuts at: 0 858 951
Size: 858 93
BsiHKAI G_wGCw'C
Cuts at: 0 830 951
Size: 830 121
BsmI GAATG_Cn'
Cuts at: 0 129 951
Size: 129 822
Bsp1286I G_dGCh'C
Cuts at: 0 830 951
Size: 830 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 571 951
Size: 571 380
BsrBI CCG'CTC
Cuts at: 0 727 951
Size: 727 224
BsrDI GCAATG_nn'
Cuts at: 0 662 951
Size: 662 289
BsrGI T'GTAC_A
Cuts at: 0 794 951
Size: 794 157
BstYI r'GATC_y
Cuts at: 0 680 951
Size: 680 271
Bsu36I CC'TnA_GG
Cuts at: 0 676 874 951
Size: 676 198 77
Fragments arranged by size:
676 198 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 151 951
Size: 151 800
BtsI GCAGTG_nn'
Cuts at: 0 456 653 743 951
Size: 456 197 90 208
Fragments arranged by size:
456 208 197 90
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 411 446 951
Size: 411 35 505
Fragments arranged by size:
505 411 35
EaeI y'GGCC_r
Cuts at: 0 189 371 419 951
Size: 189 182 48 532
Fragments arranged by size:
532 189 182 48
EarI CTCTTCn'nnn_
Cuts at: 0 772 951
Size: 772 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 226 444 885 951
Size: 52 174 218 441 66
Fragments arranged by size:
441 218 174 66 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 259 951
Size: 259 692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 915 951
Size: 915 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 951
Size: 8 32 911
Fragments arranged by size:
911 32 8
HindIII A'AGCT_T
Cuts at: 0 475 951
Size: 475 476
MfeI C'AATT_G
Cuts at: 0 493 951
Size: 493 458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 951
Size: 98 853
MscI TGG'CCA
Cuts at: 0 191 373 421 951
Size: 191 182 48 530
Fragments arranged by size:
530 191 182 48
MspA1I CmG'CkG
Cuts at: 0 72 951
Size: 72 879
PfoI T'CCnGG_A
Cuts at: 0 203 951
Size: 203 748
PvuII CAG'CTG
Cuts at: 0 72 951
Size: 72 879
SmaI CCC'GGG
Cuts at: 0 787 951
Size: 787 164
SnaBI TAC'GTA
Cuts at: 0 282 951
Size: 282 669
StuI AGG'CCT
Cuts at: 0 898 951
Size: 898 53
StyI C'CwwG_G
Cuts at: 0 186 934 951
Size: 186 748 17
Fragments arranged by size:
748 186 17
TatI w'GTAC_w
Cuts at: 0 176 302 325 794 951
Size: 176 126 23 469 157
Fragments arranged by size:
469 176 157 126 23
XmaI C'CCGG_G
Cuts at: 0 785 951
Size: 785 166
Enzymes that do cut:
AarI AccI AcuI ApoI AvaI AvrII BamHI BbsI
BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI BstYI Bsu36I
BtgZI BtsI CspCI EaeI EarI Eco57MI EcoNI FalI
Hin4I HindIII MfeI MmeI MscI MspA1I PfoI PvuII
SmaI SnaBI StuI StyI TatI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaI BsaBI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI ClaI DraI DraIII
DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI ZraI