Restriction map for
pFN21AE6038
(Linear) (Six-base) MAPSORT of: KIEE6038.seq Check: 3698 from: 1 to: 951
KIEE6038.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 477 516 951
Size: 477 39 435
Fragments arranged by size:
477 435 39
AflIII A'CryG_T
Cuts at: 0 730 782 951
Size: 730 52 169
Fragments arranged by size:
730 169 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 415 951
Size: 415 536
AlwNI CAG_nnn'CTG
Cuts at: 0 255 531 935 951
Size: 255 276 404 16
Fragments arranged by size:
404 276 255 16
ApoI r'AATT_y
Cuts at: 0 826 951
Size: 826 125
AvaI C'yCGr_G
Cuts at: 0 158 677 951
Size: 158 519 274
Fragments arranged by size:
519 274 158
BamHI G'GATC_C
Cuts at: 0 165 692 951
Size: 165 527 259
Fragments arranged by size:
527 259 165
BanI G'GyrC_C
Cuts at: 0 552 636 951
Size: 552 84 315
Fragments arranged by size:
552 315 84
BanII G_rGCy'C
Cuts at: 0 912 951
Size: 912 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 286 312 951
Size: 286 26 639
Fragments arranged by size:
639 286 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 462 496 951
Size: 462 34 455
Fragments arranged by size:
462 455 34
BclI T'GATC_A
Cuts at: 0 563 951
Size: 563 388
BglI GCCn_nnn'nGGC
Cuts at: 0 364 951
Size: 364 587
Bme1580I G_kGCm'C
Cuts at: 0 641 951
Size: 641 310
BmgBI CAC'GTC
Cuts at: 0 731 951
Size: 731 220
Bpu10I CC'TnA_GC
Cuts at: 0 688 951
Size: 688 263
BsaAI yAC'GTr
Cuts at: 0 381 951
Size: 381 570
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 930 951
Size: 930 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 930 951
Size: 930 21
BseYI C'CCAG_C
Cuts at: 0 330 951
Size: 330 621
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 385 505 951
Size: 285 100 120 446
Fragments arranged by size:
446 285 120 100
BsiEI CG_ry'CG
Cuts at: 0 560 951
Size: 560 391
BsiHKAI G_wGCw'C
Cuts at: 0 429 689 951
Size: 429 260 262
Fragments arranged by size:
429 262 260
BsmBI CGTCTCn'nnnn_
Cuts at: 0 759 951
Size: 759 192
Bsp1286I G_dGCh'C
Cuts at: 0 429 641 689 912 951
Size: 429 212 48 223 39
Fragments arranged by size:
429 223 212 48 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 477 516 780 951
Size: 477 39 264 171
Fragments arranged by size:
477 264 171 39
BsrFI r'CCGG_y
Cuts at: 0 555 951
Size: 555 396
BsrGI T'GTAC_A
Cuts at: 0 188 951
Size: 188 763
BssSI C'ACGA_G
Cuts at: 0 442 951
Size: 442 509
BstAPI GCAn_nnn'nTGC
Cuts at: 0 344 951
Size: 344 607
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 369 951
Size: 150 219 582
Fragments arranged by size:
582 219 150
BstYI r'GATC_y
Cuts at: 0 165 692 932 951
Size: 165 527 240 19
Fragments arranged by size:
527 240 165 19
BtgI C'CryG_G
Cuts at: 0 73 362 951
Size: 73 289 589
Fragments arranged by size:
589 289 73
BtsI GCAGTG_nn'
Cuts at: 0 689 951
Size: 689 262
DraIII CAC_nnn'GTG
Cuts at: 0 344 951
Size: 344 607
EaeI y'GGCC_r
Cuts at: 0 122 356 383 557 926 951
Size: 122 234 27 174 369 25
Fragments arranged by size:
369 234 174 122 27 25
EagI C'GGCC_G
Cuts at: 0 557 951
Size: 557 394
EarI CTCTTCn'nnn_
Cuts at: 0 766 844 951
Size: 766 78 107
Fragments arranged by size:
766 107 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 231 263 951
Size: 231 32 688
Fragments arranged by size:
688 231 32
HincII GTy'rAC
Cuts at: 0 261 951
Size: 261 690
MfeI C'AATT_G
Cuts at: 0 505 951
Size: 505 446
MscI TGG'CCA
Cuts at: 0 124 385 928 951
Size: 124 261 543 23
Fragments arranged by size:
543 261 124 23
MslI CAynn'nnrTG
Cuts at: 0 589 951
Size: 589 362
MspA1I CmG'CkG
Cuts at: 0 389 951
Size: 389 562
NaeI GCC'GGC
Cuts at: 0 557 951
Size: 557 394
NcoI C'CATG_G
Cuts at: 0 362 951
Size: 362 589
NgoMIV G'CCGG_C
Cuts at: 0 555 951
Size: 555 396
NspI r_CATG'y
Cuts at: 0 786 951
Size: 786 165
PciI A'CATG_T
Cuts at: 0 782 951
Size: 782 169
PflMI CCAn_nnn'nTGG
Cuts at: 0 330 935 951
Size: 330 605 16
Fragments arranged by size:
605 330 16
PfoI T'CCnGG_A
Cuts at: 0 215 951
Size: 215 736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 74 106 951
Size: 74 32 845
Fragments arranged by size:
845 74 32
PstI C_TGCA'G
Cuts at: 0 794 951
Size: 794 157
PvuII CAG'CTG
Cuts at: 0 389 951
Size: 389 562
SfcI C'TryA_G
Cuts at: 0 790 951
Size: 790 161
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 364 951
Size: 364 587
SmaI CCC'GGG
Cuts at: 0 160 679 951
Size: 160 519 272
Fragments arranged by size:
519 272 160
SrfI GCCC'GGGC
Cuts at: 0 679 951
Size: 679 272
StuI AGG'CCT
Cuts at: 0 721 951
Size: 721 230
StyI C'CwwG_G
Cuts at: 0 362 704 951
Size: 362 342 247
Fragments arranged by size:
362 342 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 951
Size: 46 905
TatI w'GTAC_w
Cuts at: 0 188 951
Size: 188 763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 852 884 930 951
Size: 852 32 46 21
Fragments arranged by size:
852 46 32 21
XmaI C'CCGG_G
Cuts at: 0 158 677 951
Size: 158 519 274
Fragments arranged by size:
519 274 158
Enzymes that do cut:
AarI AflIII AlfI AlwNI ApoI AvaI BamHI BanI
BanII BbsI BcgI BclI BglI Bme1580I BmgBI Bpu10I
BsaAI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BsrGI BssSI BstAPI BstXI BstYI
BtgI BtsI DraIII EaeI EagI EarI Hin4I HincII
MfeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PciI PflMI PfoI PpiI PstI PvuII SfcI SfiI
SmaI SrfI StuI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BciVI BfrBI BglII BlpI
BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaHI
BsaWI BsiWI BsmI BspEI BspHI BsrBI BsrDI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MluI MmeI NarI NdeI NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI SwaI Tth111I XbaI
XcmI XhoI XmnI ZraI