Restriction map for
pFN21AE6037
(Linear) (Six-base) MAPSORT of: KIEE6037.seq Check: 7096 from: 1 to: 951
KIEE6037.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 439 951
Size: 439 512
AfeI AGC'GCT
Cuts at: 0 712 951
Size: 712 239
AleI CACnn'nnGTG
Cuts at: 0 121 951
Size: 121 830
AlwNI CAG_nnn'CTG
Cuts at: 0 706 951
Size: 706 245
ApoI r'AATT_y
Cuts at: 0 37 129 951
Size: 37 92 822
Fragments arranged by size:
822 92 37
BanI G'GyrC_C
Cuts at: 0 427 951
Size: 427 524
BanII G_rGCy'C
Cuts at: 0 912 951
Size: 912 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 831 951
Size: 831 120
BlpI GC'TnA_GC
Cuts at: 0 699 707 951
Size: 699 8 244
Fragments arranged by size:
699 244 8
Bme1580I G_kGCm'C
Cuts at: 0 242 430 951
Size: 242 188 521
Fragments arranged by size:
521 242 188
BmtI G_CTAG'C
Cuts at: 0 568 951
Size: 568 383
BsaI GGTCTCn'nnnn_
Cuts at: 0 168 951
Size: 168 783
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 526 556 951
Size: 526 30 395
Fragments arranged by size:
526 395 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 630 798 951
Size: 70 560 168 153
Fragments arranged by size:
560 168 153 70
BseYI C'CCAG_C
Cuts at: 0 308 951
Size: 308 643
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 755 951
Size: 673 82 196
Fragments arranged by size:
673 196 82
BsiHKAI G_wGCw'C
Cuts at: 0 580 912 951
Size: 580 332 39
Fragments arranged by size:
580 332 39
Bsp1286I G_dGCh'C
Cuts at: 0 242 430 580 912 951
Size: 242 188 150 332 39
Fragments arranged by size:
332 242 188 150 39
BspHI T'CATG_A
Cuts at: 0 44 951
Size: 44 907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 439 951
Size: 439 512
BsrBI CCG'CTC
Cuts at: 0 698 951
Size: 698 253
BsrDI GCAATG_nn'
Cuts at: 0 650 951
Size: 650 301
BstEII G'GTnAC_C
Cuts at: 0 801 951
Size: 801 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 951
Size: 249 702
BtgI C'CryG_G
Cuts at: 0 362 951
Size: 362 589
BtsI GCAGTG_nn'
Cuts at: 0 682 951
Size: 682 269
DrdI GACnn_nn'nnGTC
Cuts at: 0 201 951
Size: 201 750
EaeI y'GGCC_r
Cuts at: 0 134 383 951
Size: 134 249 568
Fragments arranged by size:
568 249 134
EarI CTCTTCn'nnn_
Cuts at: 0 88 100 319 766 951
Size: 88 12 219 447 185
Fragments arranged by size:
447 219 185 88 12
EcoICRI GAG'CTC
Cuts at: 0 910 951
Size: 910 41
HaeII r_GCGC'y
Cuts at: 0 714 951
Size: 714 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 526 558 951
Size: 526 32 393
Fragments arranged by size:
526 393 32
MscI TGG'CCA
Cuts at: 0 136 385 951
Size: 136 249 566
Fragments arranged by size:
566 249 136
MslI CAynn'nnrTG
Cuts at: 0 121 145 391 655 859 951
Size: 121 24 246 264 204 92
Fragments arranged by size:
264 246 204 121 92 24
MspA1I CmG'CkG
Cuts at: 0 448 703 951
Size: 448 255 248
Fragments arranged by size:
448 255 248
NcoI C'CATG_G
Cuts at: 0 362 951
Size: 362 589
NheI G'CTAG_C
Cuts at: 0 564 951
Size: 564 387
PflMI CCAn_nnn'nTGG
Cuts at: 0 20 951
Size: 20 931
PvuII CAG'CTG
Cuts at: 0 448 703 951
Size: 448 255 248
Fragments arranged by size:
448 255 248
SacI G_AGCT'C
Cuts at: 0 912 951
Size: 912 39
SapI GCTCTTCn'nnn_
Cuts at: 0 319 951
Size: 319 632
SfcI C'TryA_G
Cuts at: 0 879 951
Size: 879 72
StuI AGG'CCT
Cuts at: 0 721 951
Size: 721 230
StyI C'CwwG_G
Cuts at: 0 119 362 797 951
Size: 119 243 435 154
Fragments arranged by size:
435 243 154 119
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 492 951
Size: 492 459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 951
Size: 144 807
XmnI GAAnn'nnTTC
Cuts at: 0 721 951
Size: 721 230
Enzymes that do cut:
AarI AfeI AleI AlwNI ApoI BanI BanII BbsI
BlpI Bme1580I BmtI BsaI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BstEII BstXI
BtgI BtsI DrdI EaeI EarI EcoICRI HaeII Hin4I
MscI MslI MspA1I NcoI NheI PflMI PvuII SacI
SapI SfcI StuI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EagI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmaI ZraI