Restriction map for
pFN21AE6035
(Linear) (Six-base) MAPSORT of: KIEE6035.seq Check: 6006 from: 1 to: 951
KIEE6035.orf
With 182 enzymes: SgfI *
December 5, 2014 13:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 477 577 951
Size: 139 338 100 374
Fragments arranged by size:
374 338 139 100
AleI CACnn'nnGTG
Cuts at: 0 841 951
Size: 841 110
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 951
Size: 406 545
AlwNI CAG_nnn'CTG
Cuts at: 0 941 951
Size: 941 10
ApoI r'AATT_y
Cuts at: 0 31 951
Size: 31 920
BanI G'GyrC_C
Cuts at: 0 511 951
Size: 511 440
BbvCI CC'TCA_GC
Cuts at: 0 904 951
Size: 904 47
BglI GCCn_nnn'nGGC
Cuts at: 0 433 951
Size: 433 518
BglII A'GATC_T
Cuts at: 0 590 951
Size: 590 361
Bme1580I G_kGCm'C
Cuts at: 0 239 951
Size: 239 712
BmrI ACTGGGnnnn_n'
Cuts at: 0 394 951
Size: 394 557
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 951
Size: 229 722
Bpu10I CC'TnA_GC
Cuts at: 0 904 951
Size: 904 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 897 951
Size: 664 233 54
Fragments arranged by size:
664 233 54
BsaAI yAC'GTr
Cuts at: 0 722 951
Size: 722 229
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 717 951
Size: 717 234
BseYI C'CCAG_C
Cuts at: 0 246 951
Size: 246 705
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 590 951
Size: 496 94 361
Fragments arranged by size:
496 361 94
BsiHKAI G_wGCw'C
Cuts at: 0 727 951
Size: 727 224
BsmI GAATG_Cn'
Cuts at: 0 340 903 951
Size: 340 563 48
Fragments arranged by size:
563 340 48
Bsp1286I G_dGCh'C
Cuts at: 0 239 727 951
Size: 239 488 224
Fragments arranged by size:
488 239 224
BspHI T'CATG_A
Cuts at: 0 41 407 623 951
Size: 41 366 216 328
Fragments arranged by size:
366 328 216 41
BsrDI GCAATG_nn'
Cuts at: 0 76 662 951
Size: 76 586 289
Fragments arranged by size:
586 289 76
BstEII G'GTnAC_C
Cuts at: 0 144 951
Size: 144 807
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 618 951
Size: 246 372 333
Fragments arranged by size:
372 333 246
BstYI r'GATC_y
Cuts at: 0 590 683 951
Size: 590 93 268
Fragments arranged by size:
590 268 93
BtgI C'CryG_G
Cuts at: 0 353 951
Size: 353 598
EaeI y'GGCC_r
Cuts at: 0 374 951
Size: 374 577
EarI CTCTTCn'nnn_
Cuts at: 0 57 88 496 951
Size: 57 31 408 455
Fragments arranged by size:
455 408 57 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 229 477 577 951
Size: 139 90 248 100 374
Fragments arranged by size:
374 248 139 100 90
EcoO109I rG'GnC_Cy
Cuts at: 0 251 911 924 951
Size: 251 660 13 27
Fragments arranged by size:
660 251 27 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 951
Size: 585 366
MscI TGG'CCA
Cuts at: 0 376 951
Size: 376 575
MslI CAynn'nnrTG
Cuts at: 0 301 616 841 951
Size: 301 315 225 110
Fragments arranged by size:
315 301 225 110
NcoI C'CATG_G
Cuts at: 0 353 951
Size: 353 598
NdeI CA'TA_TG
Cuts at: 0 360 951
Size: 360 591
PflMI CCAn_nnn'nTGG
Cuts at: 0 508 951
Size: 508 443
PfoI T'CCnGG_A
Cuts at: 0 206 951
Size: 206 745
PpuMI rG'GwC_Cy
Cuts at: 0 251 911 924 951
Size: 251 660 13 27
Fragments arranged by size:
660 251 27 13
PstI C_TGCA'G
Cuts at: 0 385 951
Size: 385 566
SanDI GG'GwC_CC
Cuts at: 0 251 951
Size: 251 700
SapI GCTCTTCn'nnn_
Cuts at: 0 88 496 951
Size: 88 408 455
Fragments arranged by size:
455 408 88
SfcI C'TryA_G
Cuts at: 0 5 381 464 870 951
Size: 5 376 83 406 81
Fragments arranged by size:
406 376 83 81 5
SmlI C'TyrA_G
Cuts at: 0 679 876 951
Size: 679 197 75
Fragments arranged by size:
679 197 75
StyI C'CwwG_G
Cuts at: 0 285 353 951
Size: 285 68 598
Fragments arranged by size:
598 285 68
TatI w'GTAC_w
Cuts at: 0 179 951
Size: 179 772
XmnI GAAnn'nnTTC
Cuts at: 0 529 951
Size: 529 422
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApoI BanI BbvCI BglI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstEII
BstXI BstYI BtgI EaeI EarI Eco57MI EcoO109I MmeI
MscI MslI NcoI NdeI PflMI PfoI PpuMI PstI
SanDI SapI SfcI SmlI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BlpI BmgBI BmtI BplI BsaI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmaI ZraI