(Linear) (Six-base) MAPSORT of: KIEE6028.seq  Check: 9868  from: 1  to: 948



KIEE6028.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:19  ..

AflIII A'CryG_T

Cuts at:      0     836     948 
   Size:        836     112
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     579     948 
   Size:        579     369
 
ApoI r'AATT_y

Cuts at:      0      12     948 
   Size:         12     936
 
AseI AT'TA_AT

Cuts at:      0     206     948 
   Size:        206     742
 
BanII G_rGCy'C

Cuts at:      0      68     948 
   Size:         68     880
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     901     948 
   Size:        901      47
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     798     948 
   Size:        798     150
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     310     948 
   Size:        310     638
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     948 
   Size:         52     896
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     438     948 
   Size:        438     510
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     799     948 
   Size:        799     149
 
BsiHKAI G_wGCw'C

Cuts at:      0      68     101     948 
   Size:         68      33     847

  Fragments arranged by size: 

                 847      68      33
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     101     948 
   Size:         68      33     847

  Fragments arranged by size: 

                 847      68      33
 
BspHI T'CATG_A

Cuts at:      0     914     948 
   Size:        914      34
 
BsrDI GCAATG_nn'

Cuts at:      0     227     760     948 
   Size:        227     533     188

  Fragments arranged by size: 

                 533     227     188
 
BsrGI T'GTAC_A

Cuts at:      0     392     948 
   Size:        392     556
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     300     948 
   Size:        300     648
 
Bsu36I CC'TnA_GG

Cuts at:      0     877     948 
   Size:        877      71
 
BtgI C'CryG_G

Cuts at:      0     113     948 
   Size:        113     835
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     599     634     948 
   Size:        599      35     314

  Fragments arranged by size: 

                 599     314      35
 
DraI TTT'AAA

Cuts at:      0     677     939     948 
   Size:        677     262       9

  Fragments arranged by size: 

                 677     262       9
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     513     948 
   Size:         85     428     435

  Fragments arranged by size: 

                 435     428      85
 
EcoICRI GAG'CTC

Cuts at:      0      66     948 
   Size:         66     882
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     948 
   Size:         52     896
 
HincII GTy'rAC

Cuts at:      0     153     948 
   Size:        153     795
 
MslI CAynn'nnrTG

Cuts at:      0     400     775     948 
   Size:        400     375     173

  Fragments arranged by size: 

                 400     375     173
 
NcoI C'CATG_G

Cuts at:      0     113     948 
   Size:        113     835
 
NdeI CA'TA_TG

Cuts at:      0     731     948 
   Size:        731     217
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     801     948 
   Size:        801     147
 
PstI C_TGCA'G

Cuts at:      0     609     948 
   Size:        609     339
 
SacI G_AGCT'C

Cuts at:      0      68     948 
   Size:         68     880
 
SapI GCTCTTCn'nnn_

Cuts at:      0     513     948 
   Size:        513     435
 
SfcI C'TryA_G

Cuts at:      0     461     605     870     904     948 
   Size:        461     144     265      34      44

  Fragments arranged by size: 

                 461     265     144      44      34
 
SspI AAT'ATT

Cuts at:      0     660     948 
   Size:        660     288
 
StyI C'CwwG_G

Cuts at:      0     113     948 
   Size:        113     835
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     310     948 
   Size:        310     638
 
TatI w'GTAC_w

Cuts at:      0     176     392     948 
   Size:        176     216     556

  Fragments arranged by size: 

                 556     216     176
 

 Enzymes that do cut:

   AflIII     AlfI     ApoI     AseI    BanII     BbsI    BciVI     BmrI
     BpmI     BsaI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI    BsrGI
    BstXI   Bsu36I     BtgI    CspCI     DraI     EarI  EcoICRI  Eco57MI
   HincII     MslI     NcoI     NdeI    PflMI     PstI     SacI     SapI
     SfcI     SspI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I    BtgZI     BtsI     ClaI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI