(Linear) (Six-base) MAPSORT of: KIEE6027.seq  Check: 922  from: 1  to: 948



KIEE6027.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:19  ..

ApoI r'AATT_y

Cuts at:      0      49     434     948 
   Size:         49     385     514

  Fragments arranged by size: 

                 514     385      49
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     660     693     948 
   Size:        660      33     255

  Fragments arranged by size: 

                 660     255      33
 
BclI T'GATC_A

Cuts at:      0     611     948 
   Size:        611     337
 
BglI GCCn_nnn'nGGC

Cuts at:      0     370     948 
   Size:        370     578
 
BglII A'GATC_T

Cuts at:      0      81     926     948 
   Size:         81     845      22

  Fragments arranged by size: 

                 845      81      22
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     309     409     948 
   Size:        309     100     539

  Fragments arranged by size: 

                 539     309     100
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     609     948 
   Size:        609     339
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     948 
   Size:         46     902
 
BsmI GAATG_Cn'

Cuts at:      0     619     948 
   Size:        619     329
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     460     948 
   Size:        460     488
 
BsrDI GCAATG_nn'

Cuts at:      0     683     948 
   Size:        683     265
 
BssSI C'ACGA_G

Cuts at:      0     421     948 
   Size:        421     527
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     261     601     948 
   Size:        261     340     347

  Fragments arranged by size: 

                 347     340     261
 
BstYI r'GATC_y

Cuts at:      0      81     926     948 
   Size:         81     845      22

  Fragments arranged by size: 

                 845      81      22
 
BtsI GCAGTG_nn'

Cuts at:      0     565     948 
   Size:        565     383
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     116     151     948 
   Size:        116      35     797

  Fragments arranged by size: 

                 797     116      35
 
DraI TTT'AAA

Cuts at:      0     893     948 
   Size:        893      55
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     835     948 
   Size:        835     113
 
EaeI y'GGCC_r

Cuts at:      0     362     948 
   Size:        362     586
 
EarI CTCTTCn'nnn_

Cuts at:      0     243     790     948 
   Size:        243     547     158

  Fragments arranged by size: 

                 547     243     158
 
EcoO109I rG'GnC_Cy

Cuts at:      0     915     948 
   Size:        915      33
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     945     948 
   Size:        945       3
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     948 
   Size:         95     853
 
MscI TGG'CCA

Cuts at:      0     364     948 
   Size:        364     584
 
MslI CAynn'nnrTG

Cuts at:      0     190     305     599     948 
   Size:        190     115     294     349

  Fragments arranged by size: 

                 349     294     190     115
 
MspA1I CmG'CkG

Cuts at:      0     644     948 
   Size:        644     304
 
NdeI CA'TA_TG

Cuts at:      0     375     948 
   Size:        375     573
 
PvuII CAG'CTG

Cuts at:      0     644     948 
   Size:        644     304
 
SfcI C'TryA_G

Cuts at:      0     885     948 
   Size:        885      63
 
SmlI C'TyrA_G

Cuts at:      0     624     948 
   Size:        624     324
 
TatI w'GTAC_w

Cuts at:      0     791     948 
   Size:        791     157
 

 Enzymes that do cut:

     ApoI     BaeI     BclI     BglI    BglII     BmrI    BpuEI    BseRI
     BsmI    BspMI    BsrDI    BssSI    BstXI    BstYI     BtsI    CspCI
     DraI     DrdI     EaeI     EarI EcoO109I     FalI     MmeI     MscI
     MslI   MspA1I     NdeI    PvuII     SfcI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI   DraIII     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI