Restriction map for
pFN21AE6021
(Linear) (Six-base) MAPSORT of: KIEE6021.seq Check: 9547 from: 1 to: 945
KIEE6021.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 670 945
Size: 583 87 275
Fragments arranged by size:
583 275 87
AflIII A'CryG_T
Cuts at: 0 727 945
Size: 727 218
AvaI C'yCGr_G
Cuts at: 0 815 945
Size: 815 130
BanII G_rGCy'C
Cuts at: 0 182 481 608 945
Size: 182 299 127 337
Fragments arranged by size:
337 299 182 127
BclI T'GATC_A
Cuts at: 0 431 945
Size: 431 514
BglI GCCn_nnn'nGGC
Cuts at: 0 205 945
Size: 205 740
Bme1580I G_kGCm'C
Cuts at: 0 815 945
Size: 815 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 448 528 945
Size: 448 80 417
Fragments arranged by size:
448 417 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 945
Size: 88 857
BsaAI yAC'GTr
Cuts at: 0 110 378 663 945
Size: 110 268 285 282
Fragments arranged by size:
285 282 268 110
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 219 222 945
Size: 133 86 3 723
Fragments arranged by size:
723 133 86 3
BseYI C'CCAG_C
Cuts at: 0 768 945
Size: 768 177
BsiEI CG_ry'CG
Cuts at: 0 428 945
Size: 428 517
BsiHKAI G_wGCw'C
Cuts at: 0 353 551 945
Size: 353 198 394
Fragments arranged by size:
394 353 198
Bsp1286I G_dGCh'C
Cuts at: 0 182 353 481 551 608 815 945
Size: 182 171 128 70 57 207 130
Fragments arranged by size:
207 182 171 130 128 70 57
BspHI T'CATG_A
Cuts at: 0 276 945
Size: 276 669
BsrFI r'CCGG_y
Cuts at: 0 204 945
Size: 204 741
BsrGI T'GTAC_A
Cuts at: 0 822 945
Size: 822 123
Bsu36I CC'TnA_GG
Cuts at: 0 696 945
Size: 696 249
EaeI y'GGCC_r
Cuts at: 0 197 206 254 801 945
Size: 197 9 48 547 144
Fragments arranged by size:
547 197 144 48 9
EarI CTCTTCn'nnn_
Cuts at: 0 30 945
Size: 30 915
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 583 670 945
Size: 88 495 87 275
Fragments arranged by size:
495 275 88 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 304 945
Size: 304 641
HindIII A'AGCT_T
Cuts at: 0 716 945
Size: 716 229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 423 945
Size: 108 315 522
Fragments arranged by size:
522 315 108
MscI TGG'CCA
Cuts at: 0 199 256 803 945
Size: 199 57 547 142
Fragments arranged by size:
547 199 142 57
MslI CAynn'nnrTG
Cuts at: 0 275 307 358 451 556 652 743 945
Size: 275 32 51 93 105 96 91 202
Fragments arranged by size:
275 202 105 96 93 91 51 32
NaeI GCC'GGC
Cuts at: 0 206 945
Size: 206 739
NgoMIV G'CCGG_C
Cuts at: 0 204 945
Size: 204 741
PflMI CCAn_nnn'nTGG
Cuts at: 0 263 945
Size: 263 682
SapI GCTCTTCn'nnn_
Cuts at: 0 30 945
Size: 30 915
SexAI A'CCwGG_T
Cuts at: 0 218 945
Size: 218 727
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 205 945
Size: 205 740
StuI AGG'CCT
Cuts at: 0 65 945
Size: 65 880
StyI C'CwwG_G
Cuts at: 0 921 945
Size: 921 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 414 462 945
Size: 414 48 483
Fragments arranged by size:
483 414 48
TatI w'GTAC_w
Cuts at: 0 822 945
Size: 822 123
Tth111I GACn'n_nGTC
Cuts at: 0 220 945
Size: 220 725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 216 928 945
Size: 114 102 712 17
Fragments arranged by size:
712 114 102 17
Enzymes that do cut:
AcuI AflIII AvaI BanII BclI BglI Bme1580I BmrI
BpmI BsaAI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BsrFI BsrGI Bsu36I EaeI EarI Eco57MI EcoNI HindIII
MmeI MscI MslI NaeI NgoMIV PflMI SapI SexAI
SfiI StuI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BsgI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MspA1I NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XhoI XmaI XmnI ZraI