(Linear) (Six-base) MAPSORT of: KIEE6017.seq  Check: 8658  from: 1  to: 945



KIEE6017.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:19  ..

AccI GT'mk_AC

Cuts at:      0     703     945 
   Size:        703     242
 
ApoI r'AATT_y

Cuts at:      0      25     861     945 
   Size:         25     836      84

  Fragments arranged by size: 

                 836      84      25
 
BamHI G'GATC_C

Cuts at:      0      51     945 
   Size:         51     894
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     321     945 
   Size:        321     624
 
BbvCI CC'TCA_GC

Cuts at:      0     323     945 
   Size:        323     622
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     395     429     945 
   Size:        395      34     516

  Fragments arranged by size: 

                 516     395      34
 
Bpu10I CC'TnA_GC

Cuts at:      0     323     655     945 
   Size:        323     332     290

  Fragments arranged by size: 

                 332     323     290
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     210     240     437     467     920     945 
   Size:        210      30     197      30     453      25

  Fragments arranged by size: 

                 453     210     197      30      30      25
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     941     945 
   Size:        941       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     945 
   Size:        367     578
 
BspHI T'CATG_A

Cuts at:      0     398     800     945 
   Size:        398     402     145

  Fragments arranged by size: 

                 402     398     145
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     128     715     945 
   Size:        128     587     230

  Fragments arranged by size: 

                 587     230     128
 
BsrGI T'GTAC_A

Cuts at:      0     453     479     945 
   Size:        453      26     466

  Fragments arranged by size: 

                 466     453      26
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     313     945 
   Size:        313     632
 
BstYI r'GATC_y

Cuts at:      0      51     945 
   Size:         51     894
 
BtsI GCAGTG_nn'

Cuts at:      0     379     945 
   Size:        379     566
 
EaeI y'GGCC_r

Cuts at:      0     365     434     945 
   Size:        365      69     511

  Fragments arranged by size: 

                 511     365      69
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     920     945 
   Size:        920      25
 
HincII GTy'rAC

Cuts at:      0     908     945 
   Size:        908      37
 
HpaI GTT'AAC

Cuts at:      0     908     945 
   Size:        908      37
 
MscI TGG'CCA

Cuts at:      0     367     436     945 
   Size:        367      69     509

  Fragments arranged by size: 

                 509     367      69
 
MslI CAynn'nnrTG

Cuts at:      0     130     805     829     945 
   Size:        130     675      24     116

  Fragments arranged by size: 

                 675     130     116      24
 
NspI r_CATG'y

Cuts at:      0     534     557     834     945 
   Size:        534      23     277     111

  Fragments arranged by size: 

                 534     277     111      23
 
PstI C_TGCA'G

Cuts at:      0     712     945 
   Size:        712     233
 
SfcI C'TryA_G

Cuts at:      0     708     881     945 
   Size:        708     173      64

  Fragments arranged by size: 

                 708     173      64
 
SphI G_CATG'C

Cuts at:      0     557     945 
   Size:        557     388
 
SspI AAT'ATT

Cuts at:      0      18     945 
   Size:         18     927
 
StuI AGG'CCT

Cuts at:      0     578     945 
   Size:        578     367
 
StyI C'CwwG_G

Cuts at:      0     155     276     945 
   Size:        155     121     669

  Fragments arranged by size: 

                 669     155     121
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     551     945 
   Size:        551     394
 
TatI w'GTAC_w

Cuts at:      0     453     479     945 
   Size:        453      26     466

  Fragments arranged by size: 

                 466     453      26
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     153     313     945 
   Size:        153     160     632

  Fragments arranged by size: 

                 632     160     153
 

 Enzymes that do cut:

     AccI     ApoI    BamHI     BbsI    BbvCI     BcgI   Bpu10I    BsaXI
    BseRI     BsgI    BspHI    BspMI    BsrGI    BstXI    BstYI     BtsI
     EaeI    Hin4I   HincII     HpaI     MscI     MslI     NspI     PstI
     SfcI     SphI     SspI     StuI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI