Restriction map for
pFN21AE6013
(Linear) (Six-base) MAPSORT of: KIEE6013.seq Check: 7659 from: 1 to: 942
KIEE6013.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AccI GT'mk_AC
Cuts at: 0 619 942
Size: 619 323
AflIII A'CryG_T
Cuts at: 0 77 942
Size: 77 865
ApaLI G'TGCA_C
Cuts at: 0 231 942
Size: 231 711
ApoI r'AATT_y
Cuts at: 0 434 942
Size: 434 508
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 166 199 942
Size: 166 33 743
Fragments arranged by size:
743 166 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 30 672 942
Size: 30 642 270
Fragments arranged by size:
642 270 30
BciVI GTATCCnnnnn_n'
Cuts at: 0 829 942
Size: 829 113
BclI T'GATC_A
Cuts at: 0 425 942
Size: 425 517
BfrBI ATG'CAT
Cuts at: 0 568 942
Size: 568 374
Bme1580I G_kGCm'C
Cuts at: 0 235 942
Size: 235 707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 680 942
Size: 227 453 262
Fragments arranged by size:
453 262 227
Bpu10I CC'TnA_GC
Cuts at: 0 249 942
Size: 249 693
BsaAI yAC'GTr
Cuts at: 0 230 942
Size: 230 712
BsaBI GATnn'nnATC
Cuts at: 0 88 942
Size: 88 854
BsaHI Gr'CG_yC
Cuts at: 0 801 942
Size: 801 141
BseYI C'CCAG_C
Cuts at: 0 48 271 942
Size: 48 223 671
Fragments arranged by size:
671 223 48
BsiHKAI G_wGCw'C
Cuts at: 0 235 942
Size: 235 707
BsmI GAATG_Cn'
Cuts at: 0 845 942
Size: 845 97
Bsp1286I G_dGCh'C
Cuts at: 0 235 942
Size: 235 707
BstBI TT'CG_AA
Cuts at: 0 582 942
Size: 582 360
BstXI CCAn_nnnn'nTGG
Cuts at: 0 796 942
Size: 796 146
BstYI r'GATC_y
Cuts at: 0 344 942
Size: 344 598
Bsu36I CC'TnA_GG
Cuts at: 0 242 942
Size: 242 700
DraI TTT'AAA
Cuts at: 0 494 942
Size: 494 448
EarI CTCTTCn'nnn_
Cuts at: 0 193 313 942
Size: 193 120 629
Fragments arranged by size:
629 193 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 680 942
Size: 227 453 262
Fragments arranged by size:
453 262 227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 521 942
Size: 521 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 467 942
Size: 435 32 475
Fragments arranged by size:
475 435 32
HincII GTy'rAC
Cuts at: 0 620 942
Size: 620 322
MslI CAynn'nnrTG
Cuts at: 0 613 942
Size: 613 329
NdeI CA'TA_TG
Cuts at: 0 382 942
Size: 382 560
NsiI A_TGCA'T
Cuts at: 0 570 942
Size: 570 372
NspI r_CATG'y
Cuts at: 0 81 201 572 690 942
Size: 81 120 371 118 252
Fragments arranged by size:
371 252 120 118 81
PciI A'CATG_T
Cuts at: 0 77 942
Size: 77 865
PflMI CCAn_nnn'nTGG
Cuts at: 0 48 942
Size: 48 894
PfoI T'CCnGG_A
Cuts at: 0 902 942
Size: 902 40
PmlI CAC'GTG
Cuts at: 0 230 942
Size: 230 712
PshAI GACnn'nnGTC
Cuts at: 0 625 942
Size: 625 317
PsiI TTA'TAA
Cuts at: 0 931 942
Size: 931 11
SalI G'TCGA_C
Cuts at: 0 618 942
Size: 618 324
SapI GCTCTTCn'nnn_
Cuts at: 0 313 942
Size: 313 629
SfcI C'TryA_G
Cuts at: 0 64 942
Size: 64 878
SphI G_CATG'C
Cuts at: 0 201 942
Size: 201 741
SspI AAT'ATT
Cuts at: 0 522 942
Size: 522 420
StuI AGG'CCT
Cuts at: 0 247 942
Size: 247 695
TatI w'GTAC_w
Cuts at: 0 639 942
Size: 639 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 803 835 942
Size: 803 32 107
Fragments arranged by size:
803 107 32
Tth111I GACn'n_nGTC
Cuts at: 0 217 942
Size: 217 725
Enzymes that do cut:
AccI AflIII ApaLI ApoI BaeI BbsI BciVI BclI
BfrBI Bme1580I BpmI Bpu10I BsaAI BsaBI BsaHI BseYI
BsiHKAI BsmI Bsp1286I BstBI BstXI BstYI Bsu36I DraI
EarI Eco57MI FalI Hin4I HincII MslI NdeI NsiI
NspI PciI PflMI PfoI PmlI PshAI PsiI SalI
SapI SfcI SphI SspI StuI TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbvCI
BcgI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PmeI PpiI PpuMI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI StyI SwaI TaqII XbaI XcmI
XhoI XmaI XmnI ZraI