Restriction map for
pFN21AE6010
(Linear) (Six-base) MAPSORT of: KIEE6010.seq Check: 5662 from: 1 to: 942
KIEE6010.orf
With 182 enzymes: SgfI *
December 5, 2014 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 942
Size: 727 215
AflIII A'CryG_T
Cuts at: 0 770 942
Size: 770 172
AgeI A'CCGG_T
Cuts at: 0 362 942
Size: 362 580
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 942
Size: 403 539
AlwNI CAG_nnn'CTG
Cuts at: 0 419 942
Size: 419 523
ApoI r'AATT_y
Cuts at: 0 680 942
Size: 680 262
BamHI G'GATC_C
Cuts at: 0 74 942
Size: 74 868
BanI G'GyrC_C
Cuts at: 0 509 589 715 942
Size: 509 80 126 227
Fragments arranged by size:
509 227 126 80
BclI T'GATC_A
Cuts at: 0 401 942
Size: 401 541
Bme1580I G_kGCm'C
Cuts at: 0 512 900 942
Size: 512 388 42
Fragments arranged by size:
512 388 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 52 910 942
Size: 52 858 32
Fragments arranged by size:
858 52 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 942
Size: 88 854
BsaI GGTCTCn'nnnn_
Cuts at: 0 747 942
Size: 747 195
BsaWI w'CCGG_w
Cuts at: 0 362 554 942
Size: 362 192 388
Fragments arranged by size:
388 362 192
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 841 942
Size: 841 101
BseYI C'CCAG_C
Cuts at: 0 236 942
Size: 236 706
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 942
Size: 373 569
Bsp1286I G_dGCh'C
Cuts at: 0 512 900 942
Size: 512 388 42
Fragments arranged by size:
512 388 42
BspEI T'CCGG_A
Cuts at: 0 554 942
Size: 554 388
BspHI T'CATG_A
Cuts at: 0 404 942
Size: 404 538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 942
Size: 727 215
BsrFI r'CCGG_y
Cuts at: 0 362 942
Size: 362 580
BsrGI T'GTAC_A
Cuts at: 0 773 942
Size: 773 169
BssSI C'ACGA_G
Cuts at: 0 514 942
Size: 514 428
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 468 942
Size: 138 330 474
Fragments arranged by size:
474 330 138
BstYI r'GATC_y
Cuts at: 0 74 942
Size: 74 868
DraIII CAC_nnn'GTG
Cuts at: 0 519 942
Size: 519 423
DrdI GACnn_nn'nnGTC
Cuts at: 0 592 942
Size: 592 350
EaeI y'GGCC_r
Cuts at: 0 458 470 578 942
Size: 458 12 108 364
Fragments arranged by size:
458 364 108 12
EarI CTCTTCn'nnn_
Cuts at: 0 307 832 916 942
Size: 307 525 84 26
Fragments arranged by size:
525 307 84 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 942
Size: 88 854
MscI TGG'CCA
Cuts at: 0 460 472 580 942
Size: 460 12 108 362
Fragments arranged by size:
460 362 108 12
MslI CAynn'nnrTG
Cuts at: 0 139 172 769 775 942
Size: 139 33 597 6 167
Fragments arranged by size:
597 167 139 33 6
MspA1I CmG'CkG
Cuts at: 0 240 503 942
Size: 240 263 439
Fragments arranged by size:
439 263 240
NspI r_CATG'y
Cuts at: 0 774 780 942
Size: 774 6 162
Fragments arranged by size:
774 162 6
PciI A'CATG_T
Cuts at: 0 770 942
Size: 770 172
PflMI CCAn_nnn'nTGG
Cuts at: 0 519 942
Size: 519 423
PfoI T'CCnGG_A
Cuts at: 0 203 942
Size: 203 739
PvuII CAG'CTG
Cuts at: 0 240 942
Size: 240 702
SapI GCTCTTCn'nnn_
Cuts at: 0 307 832 942
Size: 307 525 110
Fragments arranged by size:
525 307 110
StuI AGG'CCT
Cuts at: 0 709 942
Size: 709 233
StyI C'CwwG_G
Cuts at: 0 467 629 942
Size: 467 162 313
Fragments arranged by size:
467 313 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 71 310 601 674 942
Size: 37 34 239 291 73 268
Fragments arranged by size:
291 268 239 73 37 34
TatI w'GTAC_w
Cuts at: 0 176 773 942
Size: 176 597 169
Fragments arranged by size:
597 176 169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 135 279 942
Size: 135 144 663
Fragments arranged by size:
663 144 135
XmnI GAAnn'nnTTC
Cuts at: 0 709 942
Size: 709 233
Enzymes that do cut:
AarI AflIII AgeI AlfI AlwNI ApoI BamHI BanI
BclI Bme1580I BmrI BpmI BsaI BsaWI BseRI BseYI
BsgI Bsp1286I BspEI BspHI BspMI BsrFI BsrGI BssSI
BstXI BstYI DraIII DrdI EaeI EarI Eco57MI MscI
MslI MspA1I NspI PciI PflMI PfoI PvuII SapI
StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI BsiWI
BsmI BsmBI BsrBI BsrDI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI