(Linear) (Six-base) MAPSORT of: KIEE6009.seq  Check: 47  from: 1  to: 942



KIEE6009.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     870     942 
   Size:        870      72
 
AleI CACnn'nnGTG

Cuts at:      0     217     942 
   Size:        217     725
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     942 
   Size:        397     545
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      10      42     942 
   Size:         10      32     900

  Fragments arranged by size: 

                 900      32      10
 
ApoI r'AATT_y

Cuts at:      0     673     915     942 
   Size:        673     242      27

  Fragments arranged by size: 

                 673     242      27
 
AvrII C'CTAG_G

Cuts at:      0      36     942 
   Size:         36     906
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     557     590     942 
   Size:        557      33     352

  Fragments arranged by size: 

                 557     352      33
 
BamHI G'GATC_C

Cuts at:      0     147     942 
   Size:        147     795
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     885     942 
   Size:        885      57
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     813     942 
   Size:        813     129
 
BglII A'GATC_T

Cuts at:      0     551     942 
   Size:        551     391
 
Bpu10I CC'TnA_GC

Cuts at:      0     597     942 
   Size:        597     345
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     142     469     942 
   Size:        123      19     327     473

  Fragments arranged by size: 

                 473     327     123      19
 
BsaHI Gr'CG_yC

Cuts at:      0     163     942 
   Size:        163     779
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     543     668     942 
   Size:        543     125     274

  Fragments arranged by size: 

                 543     274     125
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     870     942 
   Size:        870      72
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     413     942 
   Size:        413     529
 
BstYI r'GATC_y

Cuts at:      0     147     551     942 
   Size:        147     404     391

  Fragments arranged by size: 

                 404     391     147
 
BtsI GCAGTG_nn'

Cuts at:      0     379     942 
   Size:        379     563
 
EarI CTCTTCn'nnn_

Cuts at:      0     684     942 
   Size:        684     258
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     440     472     942 
   Size:        440      32     470

  Fragments arranged by size: 

                 470     440      32
 
MfeI C'AATT_G

Cuts at:      0     833     942 
   Size:        833     109
 
MslI CAynn'nnrTG

Cuts at:      0     217     942 
   Size:        217     725
 
NdeI CA'TA_TG

Cuts at:      0     747     942 
   Size:        747     195
 
PsiI TTA'TAA

Cuts at:      0     275     942 
   Size:        275     667
 
PstI C_TGCA'G

Cuts at:      0     551     942 
   Size:        551     391
 
SfcI C'TryA_G

Cuts at:      0     547     942 
   Size:        547     395
 
SmlI C'TyrA_G

Cuts at:      0     102     157     484     942 
   Size:        102      55     327     458

  Fragments arranged by size: 

                 458     327     102      55
 
SspI AAT'ATT

Cuts at:      0     209     942 
   Size:        209     733
 
StyI C'CwwG_G

Cuts at:      0      36     942 
   Size:         36     906
 
TatI w'GTAC_w

Cuts at:      0     426     715     942 
   Size:        426     289     227

  Fragments arranged by size: 

                 426     289     227
 

 Enzymes that do cut:

     AarI     AleI     AlfI     AloI     ApoI    AvrII     BaeI    BamHI
     BbsI    BciVI    BglII   Bpu10I    BpuEI    BsaHI     BsgI    BspMI
   BstAPI    BstYI     BtsI     EarI    Hin4I     MfeI     MslI     NdeI
     PsiI     PstI     SfcI     SmlI     SspI     StyI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BanI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI