Restriction map for
pFN21AE6008
(Linear) (Six-base) MAPSORT of: KIEE6008.seq Check: 2696 from: 1 to: 942
KIEE6008.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AflII C'TTAA_G
Cuts at: 0 670 942
Size: 670 272
AflIII A'CryG_T
Cuts at: 0 406 408 942
Size: 406 2 534
Fragments arranged by size:
534 406 2
AleI CACnn'nnGTG
Cuts at: 0 411 575 942
Size: 411 164 367
Fragments arranged by size:
411 367 164
AlwNI CAG_nnn'CTG
Cuts at: 0 281 942
Size: 281 661
ApoI r'AATT_y
Cuts at: 0 25 942
Size: 25 917
AseI AT'TA_AT
Cuts at: 0 136 942
Size: 136 806
BbsI GAAGACnn'nnnn_
Cuts at: 0 263 942
Size: 263 679
BbvCI CC'TCA_GC
Cuts at: 0 216 942
Size: 216 726
BciVI GTATCCnnnnn_n'
Cuts at: 0 685 942
Size: 685 257
BclI T'GATC_A
Cuts at: 0 128 659 833 942
Size: 128 531 174 109
Fragments arranged by size:
531 174 128 109
BfrBI ATG'CAT
Cuts at: 0 892 942
Size: 892 50
BmgBI CAC'GTC
Cuts at: 0 882 942
Size: 882 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 122 251 942
Size: 122 129 691
Fragments arranged by size:
691 129 122
Bpu10I CC'TnA_GC
Cuts at: 0 216 942
Size: 216 726
BsaAI yAC'GTr
Cuts at: 0 409 942
Size: 409 533
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 204 942
Size: 204 738
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 942
Size: 326 616
BsmI GAATG_Cn'
Cuts at: 0 462 892 894 942
Size: 462 430 2 48
Fragments arranged by size:
462 430 48 2
BspHI T'CATG_A
Cuts at: 0 896 942
Size: 896 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 565 942
Size: 565 377
BsrDI GCAATG_nn'
Cuts at: 0 728 942
Size: 728 214
BsrGI T'GTAC_A
Cuts at: 0 767 942
Size: 767 175
BstAPI GCAn_nnn'nTGC
Cuts at: 0 313 942
Size: 313 629
Bsu36I CC'TnA_GG
Cuts at: 0 868 942
Size: 868 74
BtgI C'CryG_G
Cuts at: 0 794 942
Size: 794 148
BtsI GCAGTG_nn'
Cuts at: 0 374 942
Size: 374 568
EcoRI G'AATT_C
Cuts at: 0 25 942
Size: 25 917
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 630 662 942
Size: 630 32 280
Fragments arranged by size:
630 280 32
MslI CAynn'nnrTG
Cuts at: 0 166 397 411 575 763 841 942
Size: 166 231 14 164 188 78 101
Fragments arranged by size:
231 188 166 164 101 78 14
MspA1I CmG'CkG
Cuts at: 0 220 942
Size: 220 722
NcoI C'CATG_G
Cuts at: 0 794 942
Size: 794 148
NsiI A_TGCA'T
Cuts at: 0 894 942
Size: 894 48
NspI r_CATG'y
Cuts at: 0 504 942
Size: 504 438
PmlI CAC'GTG
Cuts at: 0 409 942
Size: 409 533
PvuII CAG'CTG
Cuts at: 0 220 942
Size: 220 722
SfcI C'TryA_G
Cuts at: 0 61 273 428 854 942
Size: 61 212 155 426 88
Fragments arranged by size:
426 212 155 88 61
SmlI C'TyrA_G
Cuts at: 0 670 942
Size: 670 272
SspI AAT'ATT
Cuts at: 0 922 942
Size: 922 20
StyI C'CwwG_G
Cuts at: 0 794 942
Size: 794 148
TatI w'GTAC_w
Cuts at: 0 170 767 942
Size: 170 597 175
Fragments arranged by size:
597 175 170
XbaI T'CTAG_A
Cuts at: 0 927 942
Size: 927 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 942
Size: 792 150
XmnI GAAnn'nnTTC
Cuts at: 0 892 942
Size: 892 50
Enzymes that do cut:
AflII AflIII AleI AlwNI ApoI AseI BbsI BbvCI
BciVI BclI BfrBI BmgBI BmrI Bpu10I BsaAI BseRI
BsgI BsmI BspHI BspMI BsrDI BsrGI BstAPI Bsu36I
BtgI BtsI EcoRI Hin4I MslI MspA1I NcoI NsiI
NspI PmlI PvuII SfcI SmlI SspI StyI TatI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BcgI BglI
BglII BlpI Bme1580I BmtI BplI BpmI BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TstI Tth111I XhoI XmaI
ZraI