Restriction map for
pFN21AE6001
(Linear) (Six-base) MAPSORT of: KIEE6001.seq Check: 5473 from: 1 to: 942
KIEE6001.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 727 942
Size: 727 215
AleI CACnn'nnGTG
Cuts at: 0 22 373 838 942
Size: 22 351 465 104
Fragments arranged by size:
465 351 104 22
AlwNI CAG_nnn'CTG
Cuts at: 0 243 387 942
Size: 243 144 555
Fragments arranged by size:
555 243 144
BanI G'GyrC_C
Cuts at: 0 757 942
Size: 757 185
BbsI GAAGACnn'nnnn_
Cuts at: 0 942
Size: 942
BbvCI CC'TCA_GC
Cuts at: 0 187 942
Size: 187 755
BciVI GTATCCnnnnn_n'
Cuts at: 0 657 942
Size: 657 285
BglI GCCn_nnn'nGGC
Cuts at: 0 352 942
Size: 352 590
BmrI ACTGGGnnnn_n'
Cuts at: 0 446 942
Size: 446 496
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 92 942
Size: 92 850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 713 942
Size: 46 667 229
Fragments arranged by size:
667 229 46
Bpu10I CC'TnA_GC
Cuts at: 0 187 913 942
Size: 187 726 29
Fragments arranged by size:
726 187 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 800 830 942
Size: 800 30 112
Fragments arranged by size:
800 112 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 536 942
Size: 536 406
BseYI C'CCAG_C
Cuts at: 0 422 942
Size: 422 520
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 942
Size: 311 631
BsiEI CG_ry'CG
Cuts at: 0 156 374 942
Size: 156 218 568
Fragments arranged by size:
568 218 156
BsiHKAI G_wGCw'C
Cuts at: 0 263 830 942
Size: 263 567 112
Fragments arranged by size:
567 263 112
Bsp1286I G_dGCh'C
Cuts at: 0 263 830 942
Size: 263 567 112
Fragments arranged by size:
567 263 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 727 785 942
Size: 727 58 157
Fragments arranged by size:
727 157 58
BstBI TT'CG_AA
Cuts at: 0 474 942
Size: 474 468
BstEII G'GTnAC_C
Cuts at: 0 840 942
Size: 840 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 691 942
Size: 691 251
BstYI r'GATC_y
Cuts at: 0 680 942
Size: 680 262
BtgI C'CryG_G
Cuts at: 0 147 942
Size: 147 795
BtsI GCAGTG_nn'
Cuts at: 0 734 942
Size: 734 208
EaeI y'GGCC_r
Cuts at: 0 344 371 668 942
Size: 344 27 297 274
Fragments arranged by size:
344 297 274 27
EagI C'GGCC_G
Cuts at: 0 371 942
Size: 371 571
EarI CTCTTCn'nnn_
Cuts at: 0 85 313 942
Size: 85 228 629
Fragments arranged by size:
629 228 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 213 942
Size: 213 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 713 942
Size: 46 667 229
Fragments arranged by size:
667 229 46
EcoO109I rG'GnC_Cy
Cuts at: 0 426 942
Size: 426 516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 87 119 942
Size: 87 32 823
Fragments arranged by size:
823 87 32
MscI TGG'CCA
Cuts at: 0 346 670 942
Size: 346 324 272
Fragments arranged by size:
346 324 272
MslI CAynn'nnrTG
Cuts at: 0 22 130 172 373 769 838 942
Size: 22 108 42 201 396 69 104
Fragments arranged by size:
396 201 108 104 69 42 22
MspA1I CmG'CkG
Cuts at: 0 422 942
Size: 422 520
PstI C_TGCA'G
Cuts at: 0 680 942
Size: 680 262
PvuI CG_AT'CG
Cuts at: 0 156 942
Size: 156 786
SfcI C'TryA_G
Cuts at: 0 676 867 942
Size: 676 191 75
Fragments arranged by size:
676 191 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 942
Size: 352 590
StyI C'CwwG_G
Cuts at: 0 200 942
Size: 200 742
TatI w'GTAC_w
Cuts at: 0 176 942
Size: 176 766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 800 832 942
Size: 800 32 110
Fragments arranged by size:
800 110 32
Enzymes that do cut:
AarI AleI AlwNI BanI BbsI BbvCI BciVI BglI
BmrI BplI BpmI Bpu10I BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BstBI BstEII BstXI BstYI
BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoO109I
Hin4I MscI MslI MspA1I PstI PvuI SfcI SfiI
StyI TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BcgI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI