(Linear) (Six-base) MAPSORT of: KIEE5992.seq  Check: 6841  from: 1  to: 942



KIEE5992.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:18  ..

AccI GT'mk_AC

Cuts at:      0     212     942 
   Size:        212     730
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728     942 
   Size:        728     214
 
AflIII A'CryG_T

Cuts at:      0      23     942 
   Size:         23     919
 
AleI CACnn'nnGTG

Cuts at:      0     406     942 
   Size:        406     536
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     445     942 
   Size:        445     497
 
ApoI r'AATT_y

Cuts at:      0       4     942 
   Size:          4     938
 
AseI AT'TA_AT

Cuts at:      0     527     587     942 
   Size:        527      60     355

  Fragments arranged by size: 

                 527     355      60
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     718     942 
   Size:        718     224
 
BclI T'GATC_A

Cuts at:      0     149     942 
   Size:        149     793
 
BglI GCCn_nnn'nGGC

Cuts at:      0     358     942 
   Size:        358     584
 
Bme1580I G_kGCm'C

Cuts at:      0     300     942 
   Size:        300     642
 
Bpu10I CC'TnA_GC

Cuts at:      0     193     942 
   Size:        193     749
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     589     760     942 
   Size:        589     171     182

  Fragments arranged by size: 

                 589     182     171
 
BseYI C'CCAG_C

Cuts at:      0     790     942 
   Size:        790     152
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     555     942 
   Size:        555     387
 
Bsp1286I G_dGCh'C

Cuts at:      0     300     942 
   Size:        300     642
 
BsrBI CCG'CTC

Cuts at:      0     692     942 
   Size:        692     250
 
BsrGI T'GTAC_A

Cuts at:      0     398     942 
   Size:        398     544
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     249     942 
   Size:        249     693
 
BstYI r'GATC_y

Cuts at:      0     686     942 
   Size:        686     256
 
BtgI C'CryG_G

Cuts at:      0     356     942 
   Size:        356     586
 
EaeI y'GGCC_r

Cuts at:      0     350     942 
   Size:        350     592
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728     942 
   Size:        728     214
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     732     942 
   Size:        732     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     147     179     259     291     322     354     942 
   Size:        147      32      80      32      31      32     588

  Fragments arranged by size: 

                 588     147      80      32      32      32      31
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     942 
   Size:         82     860
 
MslI CAynn'nnrTG

Cuts at:      0     406     427     853     942 
   Size:        406      21     426      89

  Fragments arranged by size: 

                 426     406      89      21
 
MspA1I CmG'CkG

Cuts at:      0     907     942 
   Size:        907      35
 
NcoI C'CATG_G

Cuts at:      0     356     942 
   Size:        356     586
 
NspI r_CATG'y

Cuts at:      0     728     942 
   Size:        728     214
 
PstI C_TGCA'G

Cuts at:      0      77     305     942 
   Size:         77     228     637

  Fragments arranged by size: 

                 637     228      77
 
PvuII CAG'CTG

Cuts at:      0     907     942 
   Size:        907      35
 
SfcI C'TryA_G

Cuts at:      0      73     301     942 
   Size:         73     228     641

  Fragments arranged by size: 

                 641     228      73
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     358     942 
   Size:        358     584
 
SspI AAT'ATT

Cuts at:      0     508     942 
   Size:        508     434
 
StyI C'CwwG_G

Cuts at:      0     356     453     759     942 
   Size:        356      97     306     183

  Fragments arranged by size: 

                 356     306     183      97
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     281     942 
   Size:        281     661
 
TatI w'GTAC_w

Cuts at:      0     319     398     942 
   Size:        319      79     544

  Fragments arranged by size: 

                 544     319      79
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI     AlfI     ApoI     AseI     BbsI
     BclI     BglI Bme1580I   Bpu10I    BseRI    BseYI     BsgI Bsp1286I
    BsrBI    BsrGI    BstXI    BstYI     BtgI     EaeI  Eco57MI     FalI
    Hin4I     MmeI     MslI   MspA1I     NcoI     NspI     PstI    PvuII
     SfcI     SfiI     SspI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI