Restriction map for
pFN21AE5991
(Linear) (Six-base) MAPSORT of: KIEE5991.seq Check: 367 from: 1 to: 942
KIEE5991.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AflIII A'CryG_T
Cuts at: 0 52 942
Size: 52 890
AleI CACnn'nnGTG
Cuts at: 0 22 942
Size: 22 920
AlwNI CAG_nnn'CTG
Cuts at: 0 900 942
Size: 900 42
ApaLI G'TGCA_C
Cuts at: 0 717 942
Size: 717 225
AseI AT'TA_AT
Cuts at: 0 818 942
Size: 818 124
AvaI C'yCGr_G
Cuts at: 0 59 942
Size: 59 883
BanII G_rGCy'C
Cuts at: 0 649 942
Size: 649 293
BbsI GAAGACnn'nnnn_
Cuts at: 0 81 942
Size: 81 861
BglII A'GATC_T
Cuts at: 0 299 942
Size: 299 643
Bme1580I G_kGCm'C
Cuts at: 0 721 942
Size: 721 221
BmgBI CAC'GTC
Cuts at: 0 722 942
Size: 722 220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 529 942
Size: 88 441 413
Fragments arranged by size:
441 413 88
BsaAI yAC'GTr
Cuts at: 0 716 942
Size: 716 226
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 921 942
Size: 921 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 549 942
Size: 373 176 393
Fragments arranged by size:
393 373 176
BsiHKAI G_wGCw'C
Cuts at: 0 545 721 942
Size: 545 176 221
Fragments arranged by size:
545 221 176
BsmI GAATG_Cn'
Cuts at: 0 462 942
Size: 462 480
Bsp1286I G_dGCh'C
Cuts at: 0 545 649 721 942
Size: 545 104 72 221
Fragments arranged by size:
545 221 104 72
BspHI T'CATG_A
Cuts at: 0 593 785 942
Size: 593 192 157
Fragments arranged by size:
593 192 157
BsrFI r'CCGG_y
Cuts at: 0 776 942
Size: 776 166
BstAPI GCAn_nnn'nTGC
Cuts at: 0 900 942
Size: 900 42
BstEII G'GTnAC_C
Cuts at: 0 615 942
Size: 615 327
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 319 519 942
Size: 243 76 200 423
Fragments arranged by size:
423 243 200 76
BstYI r'GATC_y
Cuts at: 0 249 299 676 942
Size: 249 50 377 266
Fragments arranged by size:
377 266 249 50
BtgI C'CryG_G
Cuts at: 0 797 942
Size: 797 145
DraIII CAC_nnn'GTG
Cuts at: 0 396 753 942
Size: 396 357 189
Fragments arranged by size:
396 357 189
EarI CTCTTCn'nnn_
Cuts at: 0 749 942
Size: 749 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 711 942
Size: 711 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 529 942
Size: 88 441 413
Fragments arranged by size:
441 413 88
EcoO109I rG'GnC_Cy
Cuts at: 0 705 942
Size: 705 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 868 900 942
Size: 241 32 595 32 42
Fragments arranged by size:
595 241 42 32 32
MslI CAynn'nnrTG
Cuts at: 0 20 22 172 664 733 942
Size: 20 2 150 492 69 209
Fragments arranged by size:
492 209 150 69 20 2
NaeI GCC'GGC
Cuts at: 0 778 942
Size: 778 164
NcoI C'CATG_G
Cuts at: 0 797 942
Size: 797 145
NgoMIV G'CCGG_C
Cuts at: 0 776 942
Size: 776 166
NspI r_CATG'y
Cuts at: 0 732 942
Size: 732 210
PflMI CCAn_nnn'nTGG
Cuts at: 0 732 942
Size: 732 210
PfoI T'CCnGG_A
Cuts at: 0 41 203 942
Size: 41 162 739
Fragments arranged by size:
739 162 41
PmlI CAC'GTG
Cuts at: 0 716 942
Size: 716 226
PstI C_TGCA'G
Cuts at: 0 905 942
Size: 905 37
SfcI C'TryA_G
Cuts at: 0 901 942
Size: 901 41
StyI C'CwwG_G
Cuts at: 0 428 797 942
Size: 428 369 145
Fragments arranged by size:
428 369 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 411 435 942
Size: 411 24 507
Fragments arranged by size:
507 411 24
TatI w'GTAC_w
Cuts at: 0 176 942
Size: 176 766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 319 942
Size: 243 76 623
Fragments arranged by size:
623 243 76
Enzymes that do cut:
AflIII AleI AlwNI ApaLI AseI AvaI BanII BbsI
BglII Bme1580I BmgBI BpmI BsaAI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrFI BstAPI BstEII BstXI BstYI
BtgI DraIII EarI EciI Eco57MI EcoO109I Hin4I MslI
NaeI NcoI NgoMIV NspI PflMI PfoI PmlI PstI
SfcI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApaI ApoI AscI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NarI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI