Restriction map for
pFN21AE5988
(Linear) (Six-base) MAPSORT of: KIEE5988.seq Check: 5171 from: 1 to: 939
KIEE5988.orf
With 182 enzymes: SgfI *
December 5, 2014 13:18 ..
AatII G_ACGT'C
Cuts at: 0 512 939
Size: 512 427
Acc65I G'GTAC_C
Cuts at: 0 462 939
Size: 462 477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 939
Size: 812 127
AlwNI CAG_nnn'CTG
Cuts at: 0 692 915 939
Size: 692 223 24
Fragments arranged by size:
692 223 24
ApoI r'AATT_y
Cuts at: 0 25 580 939
Size: 25 555 359
Fragments arranged by size:
555 359 25
AvaI C'yCGr_G
Cuts at: 0 479 939
Size: 479 460
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 738 771 939
Size: 738 33 168
Fragments arranged by size:
738 168 33
BamHI G'GATC_C
Cuts at: 0 674 939
Size: 674 265
BanI G'GyrC_C
Cuts at: 0 462 939
Size: 462 477
BanII G_rGCy'C
Cuts at: 0 486 939
Size: 486 453
BbsI GAAGACnn'nnnn_
Cuts at: 0 729 939
Size: 729 210
BbvCI CC'TCA_GC
Cuts at: 0 616 939
Size: 616 323
BglII A'GATC_T
Cuts at: 0 203 939
Size: 203 736
Bme1580I G_kGCm'C
Cuts at: 0 230 939
Size: 230 709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 939
Size: 220 719
Bpu10I CC'TnA_GC
Cuts at: 0 616 939
Size: 616 323
BsaI GGTCTCn'nnnn_
Cuts at: 0 725 939
Size: 725 214
BsaHI Gr'CG_yC
Cuts at: 0 509 939
Size: 509 430
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 36 939
Size: 36 903
BseYI C'CCAG_C
Cuts at: 0 427 939
Size: 427 512
BsiHKAI G_wGCw'C
Cuts at: 0 486 939
Size: 486 453
Bsp1286I G_dGCh'C
Cuts at: 0 230 486 939
Size: 230 256 453
Fragments arranged by size:
453 256 230
BstEII G'GTnAC_C
Cuts at: 0 210 630 939
Size: 210 420 309
Fragments arranged by size:
420 309 210
BstXI CCAn_nnnn'nTGG
Cuts at: 0 427 939
Size: 427 512
BstYI r'GATC_y
Cuts at: 0 203 674 939
Size: 203 471 265
Fragments arranged by size:
471 265 203
BtgI C'CryG_G
Cuts at: 0 458 501 939
Size: 458 43 438
Fragments arranged by size:
458 438 43
BtsI GCAGTG_nn'
Cuts at: 0 230 379 939
Size: 230 149 560
Fragments arranged by size:
560 230 149
DraIII CAC_nnn'GTG
Cuts at: 0 795 939
Size: 795 144
DrdI GACnn_nn'nnGTC
Cuts at: 0 250 939
Size: 250 689
EaeI y'GGCC_r
Cuts at: 0 365 939
Size: 365 574
EcoICRI GAG'CTC
Cuts at: 0 484 939
Size: 484 455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 812 939
Size: 220 592 127
Fragments arranged by size:
592 220 127
EcoNI CCTnn'n_nnAGG
Cuts at: 0 928 939
Size: 928 11
EcoO109I rG'GnC_Cy
Cuts at: 0 66 939
Size: 66 873
EcoRI G'AATT_C
Cuts at: 0 580 939
Size: 580 359
KpnI G_GTAC'C
Cuts at: 0 466 939
Size: 466 473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 939
Size: 557 382
MscI TGG'CCA
Cuts at: 0 367 939
Size: 367 572
MslI CAynn'nnrTG
Cuts at: 0 133 397 939
Size: 133 264 542
Fragments arranged by size:
542 264 133
MspA1I CmG'CkG
Cuts at: 0 423 692 939
Size: 423 269 247
Fragments arranged by size:
423 269 247
NcoI C'CATG_G
Cuts at: 0 458 501 939
Size: 458 43 438
Fragments arranged by size:
458 438 43
PasI CC'CwG_GG
Cuts at: 0 312 939
Size: 312 627
PshAI GACnn'nnGTC
Cuts at: 0 610 939
Size: 610 329
PstI C_TGCA'G
Cuts at: 0 65 225 551 939
Size: 65 160 326 388
Fragments arranged by size:
388 326 160 65
PvuII CAG'CTG
Cuts at: 0 423 692 939
Size: 423 269 247
Fragments arranged by size:
423 269 247
SacI G_AGCT'C
Cuts at: 0 486 939
Size: 486 453
SfcI C'TryA_G
Cuts at: 0 61 221 547 747 895 939
Size: 61 160 326 200 148 44
Fragments arranged by size:
326 200 160 148 61 44
StuI AGG'CCT
Cuts at: 0 777 923 939
Size: 777 146 16
Fragments arranged by size:
777 146 16
StyI C'CwwG_G
Cuts at: 0 458 501 818 939
Size: 458 43 317 121
Fragments arranged by size:
458 317 121 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 180 392 939
Size: 166 14 212 547
Fragments arranged by size:
547 212 166 14
TatI w'GTAC_w
Cuts at: 0 170 939
Size: 170 769
XmnI GAAnn'nnTTC
Cuts at: 0 703 939
Size: 703 236
ZraI GAC'GTC
Cuts at: 0 510 939
Size: 510 429
Enzymes that do cut:
AatII Acc65I AcuI AlwNI ApoI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BglII Bme1580I BpmI Bpu10I
BsaI BsaHI BseRI BseYI BsiHKAI Bsp1286I BstEII BstXI
BstYI BtgI BtsI DraIII DrdI EaeI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI KpnI MmeI MscI MslI MspA1I
NcoI PasI PshAI PstI PvuII SacI SfcI StuI
StyI TaqII TatI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BbeI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI
EarI EciI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI