(Linear) (Six-base) MAPSORT of: KIEE5987.seq  Check: 133  from: 1  to: 939



KIEE5987.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:18  ..

AccI GT'mk_AC

Cuts at:      0     629     939 
   Size:        629     310
 
AflII C'TTAA_G

Cuts at:      0     706     939 
   Size:        706     233
 
AleI CACnn'nnGTG

Cuts at:      0     737     939 
   Size:        737     202
 
ApoI r'AATT_y

Cuts at:      0     278     307     939 
   Size:        278      29     632

  Fragments arranged by size: 

                 632     278      29
 
AseI AT'TA_AT

Cuts at:      0     818     939 
   Size:        818     121
 
BanII G_rGCy'C

Cuts at:      0     164     179     939 
   Size:        164      15     760

  Fragments arranged by size: 

                 760     164      15
 
BclI T'GATC_A

Cuts at:      0     353     746     939 
   Size:        353     393     193

  Fragments arranged by size: 

                 393     353     193
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     688     939 
   Size:        688     251
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     939 
   Size:        355     584
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     522     652     939 
   Size:        522     130     287

  Fragments arranged by size: 

                 522     287     130
 
BsaBI GATnn'nnATC

Cuts at:      0      88     939 
   Size:         88     851
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     761     939 
   Size:        761     178
 
BsiHKAI G_wGCw'C

Cuts at:      0     615     939 
   Size:        615     324
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     179     615     939 
   Size:        164      15     436     324

  Fragments arranged by size: 

                 436     324     164      15
 
BspHI T'CATG_A

Cuts at:      0     557     939 
   Size:        557     382
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     767     939 
   Size:        767     172
 
BsrDI GCAATG_nn'

Cuts at:      0     641     939 
   Size:        641     298
 
BstYI r'GATC_y

Cuts at:      0      83     939 
   Size:         83     856
 
BtsI GCAGTG_nn'

Cuts at:      0     749     939 
   Size:        749     190
 
EaeI y'GGCC_r

Cuts at:      0     776     939 
   Size:        776     163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     939 
   Size:        355     584
 
EcoRI G'AATT_C

Cuts at:      0     307     939 
   Size:        307     632
 
HincII GTy'rAC

Cuts at:      0     928     939 
   Size:        928      11
 
MfeI C'AATT_G

Cuts at:      0     869     939 
   Size:        869      70
 
MslI CAynn'nnrTG

Cuts at:      0     551     646     737     939 
   Size:        551      95      91     202

  Fragments arranged by size: 

                 551     202      95      91
 
NdeI CA'TA_TG

Cuts at:      0      21     939 
   Size:         21     918
 
NspI r_CATG'y

Cuts at:      0     108     465     805     939 
   Size:        108     357     340     134

  Fragments arranged by size: 

                 357     340     134     108
 
PshAI GACnn'nnGTC

Cuts at:      0     418     939 
   Size:        418     521
 
PstI C_TGCA'G

Cuts at:      0     103     939 
   Size:        103     836
 
ScaI AGT'ACT

Cuts at:      0     332     939 
   Size:        332     607
 
SfcI C'TryA_G

Cuts at:      0      99     939 
   Size:         99     840
 
SmlI C'TyrA_G

Cuts at:      0     501     667     706     939 
   Size:        501     166      39     233

  Fragments arranged by size: 

                 501     233     166      39
 
SphI G_CATG'C

Cuts at:      0     465     939 
   Size:        465     474
 
TatI w'GTAC_w

Cuts at:      0     182     330     675     939 
   Size:        182     148     345     264

  Fragments arranged by size: 

                 345     264     182     148
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     771     939 
   Size:         42     729     168

  Fragments arranged by size: 

                 729     168      42
 

 Enzymes that do cut:

     AccI    AflII     AleI     ApoI     AseI    BanII     BclI     BmrI
     BpmI    BpuEI    BsaBI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrDI    BstYI     BtsI     EaeI  Eco57MI    EcoRI   HincII     MfeI
     MslI     NdeI     NspI    PshAI     PstI     ScaI     SfcI     SmlI
     SphI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI