Restriction map for
pFN21AE5983
(Linear) (Six-base) MAPSORT of: KIEE5983.seq Check: 5874 from: 1 to: 939
KIEE5983.orf
With 182 enzymes: SgfI *
December 5, 2014 13:17 ..
AflIII A'CryG_T
Cuts at: 0 407 939
Size: 407 532
AleI CACnn'nnGTG
Cuts at: 0 841 939
Size: 841 98
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 939
Size: 406 533
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 91 939
Size: 59 32 848
Fragments arranged by size:
848 59 32
AlwNI CAG_nnn'CTG
Cuts at: 0 594 939
Size: 594 345
BamHI G'GATC_C
Cuts at: 0 442 939
Size: 442 497
BanI G'GyrC_C
Cuts at: 0 701 760 939
Size: 701 59 179
Fragments arranged by size:
701 179 59
BbeI G_GCGC'C
Cuts at: 0 705 939
Size: 705 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 921 939
Size: 921 18
BbvCI CC'TCA_GC
Cuts at: 0 190 939
Size: 190 749
BciVI GTATCCnnnnn_n'
Cuts at: 0 891 939
Size: 891 48
BclI T'GATC_A
Cuts at: 0 143 939
Size: 143 796
Bme1580I G_kGCm'C
Cuts at: 0 788 939
Size: 788 151
BmgBI CAC'GTC
Cuts at: 0 482 939
Size: 482 457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 564 939
Size: 564 375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 939
Size: 786 153
Bpu10I CC'TnA_GC
Cuts at: 0 190 939
Size: 190 749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 939
Size: 664 275
BsaBI GATnn'nnATC
Cuts at: 0 142 939
Size: 142 797
BsaHI Gr'CG_yC
Cuts at: 0 702 939
Size: 702 237
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 537 939
Size: 537 402
BsiHKAI G_wGCw'C
Cuts at: 0 487 939
Size: 487 452
Bsp1286I G_dGCh'C
Cuts at: 0 487 788 939
Size: 487 301 151
Fragments arranged by size:
487 301 151
BsrDI GCAATG_nn'
Cuts at: 0 230 939
Size: 230 709
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 939
Size: 246 693
BstYI r'GATC_y
Cuts at: 0 442 939
Size: 442 497
BtgI C'CryG_G
Cuts at: 0 756 839 939
Size: 756 83 100
Fragments arranged by size:
756 100 83
DraI TTT'AAA
Cuts at: 0 494 939
Size: 494 445
DrdI GACnn_nn'nnGTC
Cuts at: 0 925 939
Size: 925 14
EaeI y'GGCC_r
Cuts at: 0 98 374 939
Size: 98 276 565
Fragments arranged by size:
565 276 98
EarI CTCTTCn'nnn_
Cuts at: 0 88 775 939
Size: 88 687 164
Fragments arranged by size:
687 164 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 939
Size: 786 153
HaeII r_GCGC'y
Cuts at: 0 705 939
Size: 705 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 858 890 939
Size: 858 32 49
Fragments arranged by size:
858 49 32
HincII GTy'rAC
Cuts at: 0 405 939
Size: 405 534
KasI G'GCGC_C
Cuts at: 0 701 939
Size: 701 238
MscI TGG'CCA
Cuts at: 0 100 376 939
Size: 100 276 563
Fragments arranged by size:
563 276 100
MslI CAynn'nnrTG
Cuts at: 0 841 939
Size: 841 98
NarI GG'CG_CC
Cuts at: 0 702 939
Size: 702 237
NcoI C'CATG_G
Cuts at: 0 756 939
Size: 756 183
NspI r_CATG'y
Cuts at: 0 411 939
Size: 411 528
PciI A'CATG_T
Cuts at: 0 407 939
Size: 407 532
SfcI C'TryA_G
Cuts at: 0 870 939
Size: 870 69
SfoI GGC'GCC
Cuts at: 0 703 939
Size: 703 236
SmlI C'TyrA_G
Cuts at: 0 679 939
Size: 679 260
SspI AAT'ATT
Cuts at: 0 129 939
Size: 129 810
StyI C'CwwG_G
Cuts at: 0 470 756 939
Size: 470 286 183
Fragments arranged by size:
470 286 183
TatI w'GTAC_w
Cuts at: 0 179 297 939
Size: 179 118 642
Fragments arranged by size:
642 179 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 466 498 939
Size: 466 32 441
Fragments arranged by size:
466 441 32
XmnI GAAnn'nnTTC
Cuts at: 0 276 939
Size: 276 663
Enzymes that do cut:
AflIII AleI AlfI AloI AlwNI BamHI BanI BbeI
BbsI BbvCI BciVI BclI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaBI BsaHI BseRI BsiHKAI Bsp1286I BsrDI
BstXI BstYI BtgI DraI DrdI EaeI EarI Eco57MI
HaeII Hin4I HincII KasI MscI MslI NarI NcoI
NspI PciI SfcI SfoI SmlI SspI StyI TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanII BcgI BfrBI BglI BglII
BlpI BmrI BmtI BsaI BsaAI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI
MspA1I NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI ZraI