(Linear) (Six-base) MAPSORT of: KIEE5982.seq  Check: 6423  from: 1  to: 939



KIEE5982.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     730     939 
   Size:        730     209
 
AleI CACnn'nnGTG

Cuts at:      0      22     939 
   Size:         22     917
 
AlwNI CAG_nnn'CTG

Cuts at:      0     235     939 
   Size:        235     704
 
ApaI G_GGCC'C

Cuts at:      0     512     939 
   Size:        512     427
 
ApoI r'AATT_y

Cuts at:      0      31     939 
   Size:         31     908
 
BanII G_rGCy'C

Cuts at:      0     512     939 
   Size:        512     427
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     436     939 
   Size:        436     503
 
BfrBI ATG'CAT

Cuts at:      0     290     939 
   Size:        290     649
 
Bme1580I G_kGCm'C

Cuts at:      0     512     939 
   Size:        512     427
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     465     939 
   Size:         44     421     474

  Fragments arranged by size: 

                 474     421      44
 
Bpu10I CC'TnA_GC

Cuts at:      0     690     939 
   Size:        690     249
 
BsaAI yAC'GTr

Cuts at:      0     850     939 
   Size:        850      89
 
BsaWI w'CCGG_w

Cuts at:      0     554     939 
   Size:        554     385
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     598     939 
   Size:        598     341
 
BseYI C'CCAG_C

Cuts at:      0     319     939 
   Size:        319     620
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     421     939 
   Size:        421     518
 
Bsp1286I G_dGCh'C

Cuts at:      0     512     939 
   Size:        512     427
 
BspEI T'CCGG_A

Cuts at:      0     554     939 
   Size:        554     385
 
BspHI T'CATG_A

Cuts at:      0     404     939 
   Size:        404     535
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     730     939 
   Size:        730     209
 
BsrDI GCAATG_nn'

Cuts at:      0     290     939 
   Size:        290     649
 
BstEII G'GTnAC_C

Cuts at:      0     909     939 
   Size:        909      30
 
BtsI GCAGTG_nn'

Cuts at:      0     368     939 
   Size:        368     571
 
EarI CTCTTCn'nnn_

Cuts at:      0     225     307     939 
   Size:        225      82     632

  Fragments arranged by size: 

                 632     225      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     465     939 
   Size:         44     421     474

  Fragments arranged by size: 

                 474     421      44
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     939 
   Size:         93     846
 
MslI CAynn'nnrTG

Cuts at:      0      22     349     475     745     939 
   Size:         22     327     126     270     194

  Fragments arranged by size: 

                 327     270     194     126      22
 
NsiI A_TGCA'T

Cuts at:      0     292     939 
   Size:        292     647
 
PfoI T'CCnGG_A

Cuts at:      0     442     939 
   Size:        442     497
 
PspOMI G'GGCC_C

Cuts at:      0     508     939 
   Size:        508     431
 
SapI GCTCTTCn'nnn_

Cuts at:      0     307     939 
   Size:        307     632
 
SfcI C'TryA_G

Cuts at:      0     600     939 
   Size:        600     339
 
SnaBI TAC'GTA

Cuts at:      0     850     939 
   Size:        850      89
 
SpeI A'CTAG_T

Cuts at:      0      72     939 
   Size:         72     867
 
SspI AAT'ATT

Cuts at:      0     839     939 
   Size:        839     100
 
StyI C'CwwG_G

Cuts at:      0      20     939 
   Size:         20     919
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     536     549     568     581     939 
   Size:        536      13      19      13     358

  Fragments arranged by size: 

                 536     358      19      13      13
 
XbaI T'CTAG_A

Cuts at:      0     150     939 
   Size:        150     789
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     903     939 
   Size:        903      36
 
XmnI GAAnn'nnTTC

Cuts at:      0      35     939 
   Size:         35     904
 

 Enzymes that do cut:

     AarI     AleI    AlwNI     ApaI     ApoI    BanII    BciVI    BfrBI
 Bme1580I     BpmI   Bpu10I    BsaAI    BsaWI    BseRI    BseYI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrDI   BstEII     BtsI     EarI
  Eco57MI     MmeI     MslI     NsiI     PfoI   PspOMI     SapI     SfcI
    SnaBI     SpeI     SspI     StyI     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI     BclI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI     SphI     SrfI     StuI     SwaI    TaqII     TatI
  Tth111I     XhoI     XmaI     ZraI