Restriction map for
pFN21AE5981
(Linear) (Six-base) MAPSORT of: KIEE5981.seq Check: 230 from: 1 to: 939
KIEE5981.orf
With 182 enzymes: SgfI *
December 5, 2014 13:17 ..
AccI GT'mk_AC
Cuts at: 0 226 939
Size: 226 713
AflII C'TTAA_G
Cuts at: 0 495 939
Size: 495 444
AleI CACnn'nnGTG
Cuts at: 0 737 939
Size: 737 202
ApaLI G'TGCA_C
Cuts at: 0 73 939
Size: 73 866
BamHI G'GATC_C
Cuts at: 0 662 939
Size: 662 277
BanII G_rGCy'C
Cuts at: 0 179 939
Size: 179 760
BbsI GAAGACnn'nnnn_
Cuts at: 0 669 939
Size: 669 270
BbvCI CC'TCA_GC
Cuts at: 0 913 939
Size: 913 26
BciVI GTATCCnnnnn_n'
Cuts at: 0 64 939
Size: 64 875
BglI GCCn_nnn'nGGC
Cuts at: 0 565 939
Size: 565 374
Bme1580I G_kGCm'C
Cuts at: 0 77 939
Size: 77 862
BmrI ACTGGGnnnn_n'
Cuts at: 0 15 939
Size: 15 924
Bpu10I CC'TnA_GC
Cuts at: 0 217 913 939
Size: 217 696 26
Fragments arranged by size:
696 217 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 471 481 939
Size: 471 10 458
Fragments arranged by size:
471 458 10
BseYI C'CCAG_C
Cuts at: 0 48 249 939
Size: 48 201 690
Fragments arranged by size:
690 201 48
BsiHKAI G_wGCw'C
Cuts at: 0 77 548 638 939
Size: 77 471 90 301
Fragments arranged by size:
471 301 90 77
Bsp1286I G_dGCh'C
Cuts at: 0 77 179 548 638 939
Size: 77 102 369 90 301
Fragments arranged by size:
369 301 102 90 77
BsrDI GCAATG_nn'
Cuts at: 0 374 434 443 554 939
Size: 374 60 9 111 385
Fragments arranged by size:
385 374 111 60 9
BsrGI T'GTAC_A
Cuts at: 0 464 939
Size: 464 475
BstAPI GCAn_nnn'nTGC
Cuts at: 0 445 939
Size: 445 494
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 939
Size: 249 690
BstYI r'GATC_y
Cuts at: 0 662 939
Size: 662 277
Bsu36I CC'TnA_GG
Cuts at: 0 454 939
Size: 454 485
BtgI C'CryG_G
Cuts at: 0 356 939
Size: 356 583
BtsI GCAGTG_nn'
Cuts at: 0 786 939
Size: 786 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 705 740 939
Size: 705 35 199
Fragments arranged by size:
705 199 35
EaeI y'GGCC_r
Cuts at: 0 194 230 353 795 939
Size: 194 36 123 442 144
Fragments arranged by size:
442 194 144 123 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 506 538 939
Size: 506 32 401
Fragments arranged by size:
506 401 32
HincII GTy'rAC
Cuts at: 0 588 939
Size: 588 351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 939
Size: 182 757
MscI TGG'CCA
Cuts at: 0 196 355 797 939
Size: 196 159 442 142
Fragments arranged by size:
442 196 159 142
MslI CAynn'nnrTG
Cuts at: 0 331 737 939
Size: 331 406 202
Fragments arranged by size:
406 331 202
NcoI C'CATG_G
Cuts at: 0 356 939
Size: 356 583
PasI CC'CwG_GG
Cuts at: 0 58 939
Size: 58 881
SfcI C'TryA_G
Cuts at: 0 5 939
Size: 5 934
SmlI C'TyrA_G
Cuts at: 0 495 939
Size: 495 444
StyI C'CwwG_G
Cuts at: 0 356 939
Size: 356 583
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 34 939
Size: 34 905
TatI w'GTAC_w
Cuts at: 0 182 464 939
Size: 182 282 475
Fragments arranged by size:
475 282 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 849 881 939
Size: 849 32 58
Fragments arranged by size:
849 58 32
Enzymes that do cut:
AccI AflII AleI ApaLI BamHI BanII BbsI BbvCI
BciVI BglI Bme1580I BmrI Bpu10I BseRI BseYI BsiHKAI
Bsp1286I BsrDI BsrGI BstAPI BstXI BstYI Bsu36I BtgI
BtsI CspCI EaeI Hin4I HincII MmeI MscI MslI
NcoI PasI SfcI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BcgI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI DraI
DraIII DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI