Restriction map for
pFN21AE5979
(Linear) (Six-base) MAPSORT of: KIEE5979.seq Check: 9981 from: 1 to: 939
KIEE5979.orf
With 182 enzymes: SgfI *
December 5, 2014 13:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 939
Size: 529 410
AflIII A'CryG_T
Cuts at: 0 644 939
Size: 644 295
AlwNI CAG_nnn'CTG
Cuts at: 0 413 939
Size: 413 526
AseI AT'TA_AT
Cuts at: 0 512 939
Size: 512 427
AvrII C'CTAG_G
Cuts at: 0 249 939
Size: 249 690
BanII G_rGCy'C
Cuts at: 0 619 939
Size: 619 320
BciVI GTATCCnnnnn_n'
Cuts at: 0 682 939
Size: 682 257
BclI T'GATC_A
Cuts at: 0 147 939
Size: 147 792
BmrI ACTGGGnnnn_n'
Cuts at: 0 644 939
Size: 644 295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 76 939
Size: 30 46 863
Fragments arranged by size:
863 46 30
Bpu10I CC'TnA_GC
Cuts at: 0 613 939
Size: 613 326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 885 939
Size: 885 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 765 939
Size: 765 174
BsaAI yAC'GTr
Cuts at: 0 645 939
Size: 645 294
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 583 777 939
Size: 583 194 162
Fragments arranged by size:
583 194 162
BseYI C'CCAG_C
Cuts at: 0 45 437 939
Size: 45 392 502
Fragments arranged by size:
502 392 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 939
Size: 367 572
BsiHKAI G_wGCw'C
Cuts at: 0 16 619 939
Size: 16 603 320
Fragments arranged by size:
603 320 16
BsmI GAATG_Cn'
Cuts at: 0 625 939
Size: 625 314
Bsp1286I G_dGCh'C
Cuts at: 0 16 619 939
Size: 16 603 320
Fragments arranged by size:
603 320 16
BspHI T'CATG_A
Cuts at: 0 398 914 939
Size: 398 516 25
Fragments arranged by size:
516 398 25
BsrDI GCAATG_nn'
Cuts at: 0 530 737 939
Size: 530 207 202
Fragments arranged by size:
530 207 202
BsrGI T'GTAC_A
Cuts at: 0 647 939
Size: 647 292
BstAPI GCAn_nnn'nTGC
Cuts at: 0 413 939
Size: 413 526
BstBI TT'CG_AA
Cuts at: 0 63 939
Size: 63 876
BstEII G'GTnAC_C
Cuts at: 0 831 939
Size: 831 108
BtgI C'CryG_G
Cuts at: 0 744 939
Size: 744 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 136 939
Size: 136 803
BtsI GCAGTG_nn'
Cuts at: 0 379 939
Size: 379 560
EaeI y'GGCC_r
Cuts at: 0 809 939
Size: 809 130
EarI CTCTTCn'nnn_
Cuts at: 0 79 251 545 939
Size: 79 172 294 394
Fragments arranged by size:
394 294 172 79
EcoICRI GAG'CTC
Cuts at: 0 617 939
Size: 617 322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 76 529 939
Size: 30 46 453 410
Fragments arranged by size:
453 410 46 30
EcoO109I rG'GnC_Cy
Cuts at: 0 410 638 939
Size: 410 228 301
Fragments arranged by size:
410 301 228
EcoRV GAT'ATC
Cuts at: 0 204 939
Size: 204 735
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 509 603 939
Size: 509 94 336
Fragments arranged by size:
509 336 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 762 794 939
Size: 135 32 595 32 145
Fragments arranged by size:
595 145 135 32 32
HindIII A'AGCT_T
Cuts at: 0 698 939
Size: 698 241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 939
Size: 493 446
MslI CAynn'nnrTG
Cuts at: 0 478 760 826 939
Size: 478 282 66 113
Fragments arranged by size:
478 282 113 66
MspA1I CmG'CkG
Cuts at: 0 814 939
Size: 814 125
PasI CC'CwG_GG
Cuts at: 0 684 939
Size: 684 255
PfoI T'CCnGG_A
Cuts at: 0 53 939
Size: 53 886
PpuMI rG'GwC_Cy
Cuts at: 0 638 939
Size: 638 301
SacI G_AGCT'C
Cuts at: 0 619 939
Size: 619 320
SfcI C'TryA_G
Cuts at: 0 498 858 939
Size: 498 360 81
Fragments arranged by size:
498 360 81
SmlI C'TyrA_G
Cuts at: 0 864 939
Size: 864 75
StyI C'CwwG_G
Cuts at: 0 249 939
Size: 249 690
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 429 697 939
Size: 429 268 242
Fragments arranged by size:
429 268 242
TatI w'GTAC_w
Cuts at: 0 170 647 939
Size: 170 477 292
Fragments arranged by size:
477 292 170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 939
Size: 425 32 482
Fragments arranged by size:
482 425 32
Enzymes that do cut:
AcuI AflIII AlwNI AseI AvrII BanII BciVI BclI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstAPI
BstBI BstEII BtgI BtgZI BtsI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRV FalI Hin4I HindIII MmeI MslI
MspA1I PasI PfoI PpuMI SacI SfcI SmlI StyI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AsiSI AvaI BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrFI BssHII BssSI BstXI BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI