Restriction map for
pFN21AE5977
(Linear) (Six-base) MAPSORT of: KIEE5977.seq Check: 7043 from: 1 to: 936
KIEE5977.orf
With 182 enzymes: SgfI *
December 5, 2014 13:17 ..
ApoI r'AATT_y
Cuts at: 0 12 299 416 936
Size: 12 287 117 520
Fragments arranged by size:
520 287 117 12
AvaI C'yCGr_G
Cuts at: 0 512 656 936
Size: 512 144 280
Fragments arranged by size:
512 280 144
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 215 248 936
Size: 215 33 688
Fragments arranged by size:
688 215 33
BamHI G'GATC_C
Cuts at: 0 446 936
Size: 446 490
BanII G_rGCy'C
Cuts at: 0 412 457 936
Size: 412 45 479
Fragments arranged by size:
479 412 45
BbvCI CC'TCA_GC
Cuts at: 0 187 936
Size: 187 749
BglII A'GATC_T
Cuts at: 0 908 936
Size: 908 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 936
Size: 710 226
Bpu10I CC'TnA_GC
Cuts at: 0 187 936
Size: 187 749
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 936
Size: 515 421
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 936
Size: 529 30 377
Fragments arranged by size:
529 377 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 172 175 936
Size: 130 42 3 761
Fragments arranged by size:
761 130 42 3
BseYI C'CCAG_C
Cuts at: 0 158 936
Size: 158 778
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 936
Size: 207 729
BsmI GAATG_Cn'
Cuts at: 0 337 898 936
Size: 337 561 38
Fragments arranged by size:
561 337 38
Bsp1286I G_dGCh'C
Cuts at: 0 412 457 936
Size: 412 45 479
Fragments arranged by size:
479 412 45
BspHI T'CATG_A
Cuts at: 0 404 902 936
Size: 404 498 34
Fragments arranged by size:
498 404 34
BsrDI GCAATG_nn'
Cuts at: 0 641 936
Size: 641 295
BsrGI T'GTAC_A
Cuts at: 0 773 936
Size: 773 163
BstEII G'GTnAC_C
Cuts at: 0 888 936
Size: 888 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 279 936
Size: 279 657
BstYI r'GATC_y
Cuts at: 0 446 685 908 936
Size: 446 239 223 28
Fragments arranged by size:
446 239 223 28
BtsI GCAGTG_nn'
Cuts at: 0 316 368 573 936
Size: 316 52 205 363
Fragments arranged by size:
363 316 205 52
EaeI y'GGCC_r
Cuts at: 0 95 936
Size: 95 841
EarI CTCTTCn'nnn_
Cuts at: 0 85 936
Size: 85 851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 936
Size: 710 226
EcoRI G'AATT_C
Cuts at: 0 12 936
Size: 12 924
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 261 936
Size: 261 675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 936
Size: 529 32 375
Fragments arranged by size:
529 375 32
HindIII A'AGCT_T
Cuts at: 0 707 936
Size: 707 229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 936
Size: 53 883
MscI TGG'CCA
Cuts at: 0 97 936
Size: 97 839
MslI CAynn'nnrTG
Cuts at: 0 172 277 679 847 936
Size: 172 105 402 168 89
Fragments arranged by size:
402 172 168 105 89
PfoI T'CCnGG_A
Cuts at: 0 442 936
Size: 442 494
PspXI vC'TCGA_Gb
Cuts at: 0 656 936
Size: 656 280
SfcI C'TryA_G
Cuts at: 0 867 936
Size: 867 69
SmlI C'TyrA_G
Cuts at: 0 656 936
Size: 656 280
SspI AAT'ATT
Cuts at: 0 581 936
Size: 581 355
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 82 172 936
Size: 82 90 764
Fragments arranged by size:
764 90 82
TatI w'GTAC_w
Cuts at: 0 749 773 936
Size: 749 24 163
Fragments arranged by size:
749 163 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 105 936
Size: 105 831
XhoI C'TCGA_G
Cuts at: 0 656 936
Size: 656 280
XmnI GAAnn'nnTTC
Cuts at: 0 335 590 936
Size: 335 255 346
Fragments arranged by size:
346 335 255
Enzymes that do cut:
ApoI AvaI BaeI BamHI BanII BbvCI BglII BpmI
Bpu10I BsaI BsaXI BseRI BseYI BsgI BsmI Bsp1286I
BspHI BsrDI BsrGI BstEII BstXI BstYI BtsI EaeI
EarI Eco57MI EcoRI FalI Hin4I HindIII MmeI MscI
MslI PfoI PspXI SfcI SmlI SspI TaqII TatI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvrII BanI BbeI BbsI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI StyI SwaI TstI Tth111I XbaI XmaI
ZraI