(Linear) (Six-base) MAPSORT of: KIEE5976.seq  Check: 3220  from: 1  to: 936



KIEE5976.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:17  ..

AccI GT'mk_AC

Cuts at:      0     772     936 
   Size:        772     164
 
BanII G_rGCy'C

Cuts at:      0     412     936 
   Size:        412     524
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     648     936 
   Size:        648     288
 
BmgBI CAC'GTC

Cuts at:      0     315     936 
   Size:        315     621
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      33     936 
   Size:         33     903
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     151     181     936 
   Size:        151      30     755

  Fragments arranged by size: 

                 755     151      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64      67     936 
   Size:         64       3     869

  Fragments arranged by size: 

                 869      64       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     936 
   Size:        101     835
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     412     936 
   Size:        101     311     524

  Fragments arranged by size: 

                 524     311     101
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     319     936 
   Size:        319     617
 
BstYI r'GATC_y

Cuts at:      0      65     929     936 
   Size:         65     864       7

  Fragments arranged by size: 

                 864      65       7
 
BstZ17I GTA'TAC

Cuts at:      0     773     936 
   Size:        773     163
 
Bsu36I CC'TnA_GG

Cuts at:      0     687     936 
   Size:        687     249
 
BtgI C'CryG_G

Cuts at:      0     350     936 
   Size:        350     586
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     276     936 
   Size:        276     660
 
DraIII CAC_nnn'GTG

Cuts at:      0      21     936 
   Size:         21     915
 
EaeI y'GGCC_r

Cuts at:      0     188     778     936 
   Size:        188     590     158

  Fragments arranged by size: 

                 590     188     158
 
EcoO109I rG'GnC_Cy

Cuts at:      0     705     936 
   Size:        705     231
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     275     307     936 
   Size:        275      32     629

  Fragments arranged by size: 

                 629     275      32
 
HincII GTy'rAC

Cuts at:      0     603     936 
   Size:        603     333
 
HpaI GTT'AAC

Cuts at:      0     603     936 
   Size:        603     333
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     498     500     936 
   Size:        498       2     436

  Fragments arranged by size: 

                 498     436       2
 
MscI TGG'CCA

Cuts at:      0     190     936 
   Size:        190     746
 
MslI CAynn'nnrTG

Cuts at:      0     349     766     936 
   Size:        349     417     170

  Fragments arranged by size: 

                 417     349     170
 
NcoI C'CATG_G

Cuts at:      0     350     936 
   Size:        350     586
 
SfcI C'TryA_G

Cuts at:      0     576     936 
   Size:        576     360
 
StyI C'CwwG_G

Cuts at:      0     350     450     701     936 
   Size:        350     100     251     235

  Fragments arranged by size: 

                 350     251     235     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     774     936 
   Size:        774     162
 
TatI w'GTAC_w

Cuts at:      0     176     936 
   Size:        176     760
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     151     183     438     470     936 
   Size:        151      32     255      32     466

  Fragments arranged by size: 

                 466     255     151      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     319     936 
   Size:        319     617
 

 Enzymes that do cut:

     AccI    BanII     BbsI    BmgBI     BplI    BsaXI    BseRI  BsiHKAI
 Bsp1286I    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI   DraIII
     EaeI EcoO109I    Hin4I   HincII     HpaI     MmeI     MscI     MslI
     NcoI     SfcI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BmtI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII     BtsI
     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MfeI     MluI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI