(Linear) (Six-base) MAPSORT of: KIEE5972.seq  Check: 4561  from: 1  to: 936



KIEE5972.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     870     936 
   Size:        870      66
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     691     936 
   Size:        691     245
 
AflII C'TTAA_G

Cuts at:      0     867     936 
   Size:        867      69
 
AlwNI CAG_nnn'CTG

Cuts at:      0     437     936 
   Size:        437     499
 
ApoI r'AATT_y

Cuts at:      0      25     186     203     325     901     936 
   Size:         25     161      17     122     576      35

  Fragments arranged by size: 

                 576     161     122      35      25      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     213     936 
   Size:        213     723
 
BclI T'GATC_A

Cuts at:      0      89     122     239     936 
   Size:         89      33     117     697

  Fragments arranged by size: 

                 697     117      89      33
 
BmtI G_CTAG'C

Cuts at:      0     601     936 
   Size:        601     335
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     489     936 
   Size:        489     447
 
BseYI C'CCAG_C

Cuts at:      0     686     936 
   Size:        686     250
 
BspHI T'CATG_A

Cuts at:      0     398     936 
   Size:        398     538
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     870     936 
   Size:        870      66
 
BsrDI GCAATG_nn'

Cuts at:      0     245     936 
   Size:        245     691
 
Bsu36I CC'TnA_GG

Cuts at:      0     181     895     936 
   Size:        181     714      41

  Fragments arranged by size: 

                 714     181      41
 
BtgI C'CryG_G

Cuts at:      0     284     936 
   Size:        284     652
 
BtsI GCAGTG_nn'

Cuts at:      0     824     936 
   Size:        824     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     489     691     936 
   Size:        489     202     245

  Fragments arranged by size: 

                 489     245     202
 
EcoO109I rG'GnC_Cy

Cuts at:      0     891     936 
   Size:        891      45
 
EcoRI G'AATT_C

Cuts at:      0      25     901     936 
   Size:         25     876      35

  Fragments arranged by size: 

                 876      35      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     224     256     936 
   Size:        224      32     680

  Fragments arranged by size: 

                 680     224      32
 
HincII GTy'rAC

Cuts at:      0     137     936 
   Size:        137     799
 
HpaI GTT'AAC

Cuts at:      0     137     936 
   Size:        137     799
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     510     936 
   Size:        510     426
 
MslI CAynn'nnrTG

Cuts at:      0     130     613     763     936 
   Size:        130     483     150     173

  Fragments arranged by size: 

                 483     173     150     130
 
NcoI C'CATG_G

Cuts at:      0     284     936 
   Size:        284     652
 
NdeI CA'TA_TG

Cuts at:      0     725     936 
   Size:        725     211
 
NheI G'CTAG_C

Cuts at:      0     597     936 
   Size:        597     339
 
SfcI C'TryA_G

Cuts at:      0     251     936 
   Size:        251     685
 
SmlI C'TyrA_G

Cuts at:      0     867     936 
   Size:        867      69
 
SspI AAT'ATT

Cuts at:      0     150     917     936 
   Size:        150     767      19

  Fragments arranged by size: 

                 767     150      19
 
StyI C'CwwG_G

Cuts at:      0     284     629     936 
   Size:        284     345     307

  Fragments arranged by size: 

                 345     307     284
 
XbaI T'CTAG_A

Cuts at:      0     576     936 
   Size:        576     360
 
XmnI GAAnn'nnTTC

Cuts at:      0      29     329     936 
   Size:         29     300     607

  Fragments arranged by size: 

                 607     300      29
 

 Enzymes that do cut:

     AarI     AcuI    AflII    AlwNI     ApoI     BbsI     BclI     BmtI
     BpmI    BseYI    BspHI    BspMI    BsrDI   Bsu36I     BtgI     BtsI
  Eco57MI EcoO109I    EcoRI    Hin4I   HincII     HpaI     MmeI     MslI
     NcoI     NdeI     NheI     SfcI     SmlI     SspI     StyI     XbaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XcmI
     XhoI     XmaI     ZraI