Restriction map for
pFN21AE5971
(Linear) (Six-base) MAPSORT of: KIEE5971.seq Check: 5724 from: 1 to: 936
KIEE5971.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 170 541 727 936
Size: 170 371 186 209
Fragments arranged by size:
371 209 186 170
Acc65I G'GTAC_C
Cuts at: 0 392 936
Size: 392 544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 936
Size: 574 362
AflIII A'CryG_T
Cuts at: 0 572 936
Size: 572 364
AleI CACnn'nnGTG
Cuts at: 0 172 229 936
Size: 172 57 707
Fragments arranged by size:
707 172 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 657 936
Size: 657 279
AlwNI CAG_nnn'CTG
Cuts at: 0 701 936
Size: 701 235
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 376 409 936
Size: 376 33 527
Fragments arranged by size:
527 376 33
BanI G'GyrC_C
Cuts at: 0 392 681 757 936
Size: 392 289 76 179
Fragments arranged by size:
392 289 179 76
BanII G_rGCy'C
Cuts at: 0 325 607 936
Size: 325 282 329
Fragments arranged by size:
329 325 282
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 593 810 936
Size: 274 319 217 126
Fragments arranged by size:
319 274 217 126
BglI GCCn_nnn'nGGC
Cuts at: 0 136 352 936
Size: 136 216 584
Fragments arranged by size:
584 216 136
BlpI GC'TnA_GC
Cuts at: 0 93 936
Size: 93 843
Bme1580I G_kGCm'C
Cuts at: 0 686 936
Size: 686 250
BmgBI CAC'GTC
Cuts at: 0 575 936
Size: 575 361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 915 936
Size: 88 827 21
Fragments arranged by size:
827 88 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 936
Size: 445 491
BsaAI yAC'GTr
Cuts at: 0 399 516 936
Size: 399 117 420
Fragments arranged by size:
420 399 117
BsaBI GATnn'nnATC
Cuts at: 0 481 936
Size: 481 455
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 448 936
Size: 448 488
BseYI C'CCAG_C
Cuts at: 0 60 936
Size: 60 876
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 936
Size: 148 788
BsiEI CG_ry'CG
Cuts at: 0 692 936
Size: 692 244
BsiHKAI G_wGCw'C
Cuts at: 0 94 404 936
Size: 94 310 532
Fragments arranged by size:
532 310 94
BsmI GAATG_Cn'
Cuts at: 0 634 936
Size: 634 302
Bsp1286I G_dGCh'C
Cuts at: 0 94 325 404 607 686 936
Size: 94 231 79 203 79 250
Fragments arranged by size:
250 231 203 94 79 79
BspHI T'CATG_A
Cuts at: 0 404 936
Size: 404 532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 541 727 936
Size: 170 371 186 209
Fragments arranged by size:
371 209 186 170
BsrBI CCG'CTC
Cuts at: 0 9 936
Size: 9 927
BsrFI r'CCGG_y
Cuts at: 0 413 936
Size: 413 523
BstEII G'GTnAC_C
Cuts at: 0 108 936
Size: 108 828
BstXI CCAn_nnnn'nTGG
Cuts at: 0 105 723 936
Size: 105 618 213
Fragments arranged by size:
618 213 105
BstYI r'GATC_y
Cuts at: 0 539 936
Size: 539 397
Bsu36I CC'TnA_GG
Cuts at: 0 676 874 936
Size: 676 198 62
Fragments arranged by size:
676 198 62
BtgI C'CryG_G
Cuts at: 0 317 326 836 936
Size: 317 9 510 100
Fragments arranged by size:
510 317 100 9
BtsI GCAGTG_nn'
Cuts at: 0 554 936
Size: 554 382
DraIII CAC_nnn'GTG
Cuts at: 0 332 516 936
Size: 332 184 420
Fragments arranged by size:
420 332 184
EaeI y'GGCC_r
Cuts at: 0 344 371 507 665 689 936
Size: 344 27 136 158 24 247
Fragments arranged by size:
344 247 158 136 27 24
EagI C'GGCC_G
Cuts at: 0 689 936
Size: 689 247
EarI CTCTTCn'nnn_
Cuts at: 0 85 936
Size: 85 851
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 574 915 936
Size: 88 486 341 21
Fragments arranged by size:
486 341 88 21
EcoO109I rG'GnC_Cy
Cuts at: 0 447 936
Size: 447 489
EcoRV GAT'ATC
Cuts at: 0 156 936
Size: 156 780
KpnI G_GTAC'C
Cuts at: 0 396 936
Size: 396 540
MscI TGG'CCA
Cuts at: 0 346 373 509 667 936
Size: 346 27 136 158 269
Fragments arranged by size:
346 269 158 136 27
MslI CAynn'nnrTG
Cuts at: 0 139 172 229 298 487 769 847 936
Size: 139 33 57 69 189 282 78 89
Fragments arranged by size:
282 189 139 89 78 69 57 33
NcoI C'CATG_G
Cuts at: 0 836 936
Size: 836 100
NspI r_CATG'y
Cuts at: 0 566 936
Size: 566 370
PflMI CCAn_nnn'nTGG
Cuts at: 0 516 936
Size: 516 420
PfoI T'CCnGG_A
Cuts at: 0 262 936
Size: 262 674
PmlI CAC'GTG
Cuts at: 0 399 516 936
Size: 399 117 420
Fragments arranged by size:
420 399 117
PpuMI rG'GwC_Cy
Cuts at: 0 447 936
Size: 447 489
SapI GCTCTTCn'nnn_
Cuts at: 0 85 936
Size: 85 851
SexAI A'CCwGG_T
Cuts at: 0 104 936
Size: 104 832
SfcI C'TryA_G
Cuts at: 0 737 867 936
Size: 737 130 69
Fragments arranged by size:
737 130 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 936
Size: 352 584
SmlI C'TyrA_G
Cuts at: 0 460 936
Size: 460 476
SphI G_CATG'C
Cuts at: 0 566 936
Size: 566 370
StyI C'CwwG_G
Cuts at: 0 836 936
Size: 836 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 567 936
Size: 567 369
TatI w'GTAC_w
Cuts at: 0 176 773 936
Size: 176 597 163
Fragments arranged by size:
597 176 163
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI BaeI
BanI BanII BbsI BglI BlpI Bme1580I BmgBI BpmI
BpuEI BsaAI BsaBI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
Eco57MI EcoO109I EcoRV KpnI MscI MslI NcoI NspI
PflMI PfoI PmlI PpuMI SapI SexAI SfcI SfiI
SmlI SphI StyI TaqII TatI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI Bpu10I BsaI BsaHI BsaWI BsaXI
BsiWI BsmBI BspEI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI