(Linear) (Six-base) MAPSORT of: KIEE5970.seq  Check: 1146  from: 1  to: 936



KIEE5970.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:16  ..

AccI GT'mk_AC

Cuts at:      0     671     936 
   Size:        671     265
 
AleI CACnn'nnGTG

Cuts at:      0     736     936 
   Size:        736     200
 
ApaI G_GGCC'C

Cuts at:      0     897     936 
   Size:        897      39
 
BanII G_rGCy'C

Cuts at:      0     897     936 
   Size:        897      39
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     502     936 
   Size:        502     434
 
Bme1580I G_kGCm'C

Cuts at:      0     897     936 
   Size:        897      39
 
BmtI G_CTAG'C

Cuts at:      0     559     936 
   Size:        559     377
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     546     936 
   Size:        546     390
 
BsiHKAI G_wGCw'C

Cuts at:      0     471     589     936 
   Size:        471     118     347

  Fragments arranged by size: 

                 471     347     118
 
Bsp1286I G_dGCh'C

Cuts at:      0     471     589     897     936 
   Size:        471     118     308      39

  Fragments arranged by size: 

                 471     308     118      39
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     760     936 
   Size:        760     176
 
BstYI r'GATC_y

Cuts at:      0     436     784     936 
   Size:        436     348     152

  Fragments arranged by size: 

                 436     348     152
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     574     936 
   Size:        574     362
 
EaeI y'GGCC_r

Cuts at:      0     368     651     936 
   Size:        368     283     285

  Fragments arranged by size: 

                 368     285     283
 
EcoO109I rG'GnC_Cy

Cuts at:      0     893     894     936 
   Size:        893       1      42

  Fragments arranged by size: 

                 893      42       1
 
HincII GTy'rAC

Cuts at:      0     551     936 
   Size:        551     385
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     823     936 
   Size:        823     113
 
MscI TGG'CCA

Cuts at:      0     370     936 
   Size:        370     566
 
MslI CAynn'nnrTG

Cuts at:      0     169     736     844     936 
   Size:        169     567     108      92

  Fragments arranged by size: 

                 567     169     108      92
 
NheI G'CTAG_C

Cuts at:      0     555     936 
   Size:        555     381
 
NspI r_CATG'y

Cuts at:      0     644     681     936 
   Size:        644      37     255

  Fragments arranged by size: 

                 644     255      37
 
PspOMI G'GGCC_C

Cuts at:      0     893     936 
   Size:        893      43
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     627     659     936 
   Size:        627      32     277

  Fragments arranged by size: 

                 627     277      32
 
PstI C_TGCA'G

Cuts at:      0     690     936 
   Size:        690     246
 
SfcI C'TryA_G

Cuts at:      0     686     717     734     864     936 
   Size:        686      31      17     130      72

  Fragments arranged by size: 

                 686     130      72      31      17
 
SphI G_CATG'C

Cuts at:      0     681     936 
   Size:        681     255
 
StuI AGG'CCT

Cuts at:      0     706     936 
   Size:        706     230
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     739     936 
   Size:        739     197
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     213     245     476     508     936 
   Size:        213      32     231      32     428

  Fragments arranged by size: 

                 428     231     213      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     919     936 
   Size:        919      17
 

 Enzymes that do cut:

     AccI     AleI     ApaI    BanII    BciVI Bme1580I     BmtI     BsgI
  BsiHKAI Bsp1286I   BstAPI    BstYI     DrdI     EaeI EcoO109I   HincII
     MmeI     MscI     MslI     NheI     NspI   PspOMI     PsrI     PstI
     SfcI     SphI     StuI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StyI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI