(Linear) (Six-base) MAPSORT of: KIEE5968.seq  Check: 659  from: 1  to: 936



KIEE5968.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:16  ..

AccI GT'mk_AC

Cuts at:      0     671     764     936 
   Size:        671      93     172

  Fragments arranged by size: 

                 671     172      93
 
AflII C'TTAA_G

Cuts at:      0     232     936 
   Size:        232     704
 
ApaI G_GGCC'C

Cuts at:      0     897     936 
   Size:        897      39
 
BanII G_rGCy'C

Cuts at:      0     897     936 
   Size:        897      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     816     936 
   Size:        816     120
 
Bme1580I G_kGCm'C

Cuts at:      0     589     897     936 
   Size:        589     308      39

  Fragments arranged by size: 

                 589     308      39
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     290     936 
   Size:        290     646
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     825     855     936 
   Size:        825      30      81

  Fragments arranged by size: 

                 825      81      30
 
BseYI C'CCAG_C

Cuts at:      0     540     936 
   Size:        540     396
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     936 
   Size:        344     592
 
BsiHKAI G_wGCw'C

Cuts at:      0     471     936 
   Size:        471     465
 
Bsp1286I G_dGCh'C

Cuts at:      0     471     589     897     936 
   Size:        471     118     308      39

  Fragments arranged by size: 

                 471     308     118      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     724     936 
   Size:        724     212
 
BsrDI GCAATG_nn'

Cuts at:      0     365     551     629     936 
   Size:        365     186      78     307

  Fragments arranged by size: 

                 365     307     186      78
 
BssSI C'ACGA_G

Cuts at:      0     616     902     936 
   Size:        616     286      34

  Fragments arranged by size: 

                 616     286      34
 
BstYI r'GATC_y

Cuts at:      0     784     936 
   Size:        784     152
 
Bsu36I CC'TnA_GG

Cuts at:      0     871     936 
   Size:        871      65
 
DraIII CAC_nnn'GTG

Cuts at:      0     284     936 
   Size:        284     652
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     574     936 
   Size:        574     362
 
EaeI y'GGCC_r

Cuts at:      0     651     936 
   Size:        651     285
 
EarI CTCTTCn'nnn_

Cuts at:      0      82     389     936 
   Size:         82     307     547

  Fragments arranged by size: 

                 547     307      82
 
EcoO109I rG'GnC_Cy

Cuts at:      0     893     894     936 
   Size:        893       1      42

  Fragments arranged by size: 

                 893      42       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     352     936 
   Size:        352     584
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     238     270     936 
   Size:        238      32     666

  Fragments arranged by size: 

                 666     238      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     823     936 
   Size:        823     113
 
MslI CAynn'nnrTG

Cuts at:      0     169     400     844     936 
   Size:        169     231     444      92

  Fragments arranged by size: 

                 444     231     169      92
 
PspOMI G'GGCC_C

Cuts at:      0     893     936 
   Size:        893      43
 
PstI C_TGCA'G

Cuts at:      0     690     721     936 
   Size:        690      31     215

  Fragments arranged by size: 

                 690     215      31
 
SfcI C'TryA_G

Cuts at:      0     686     717     734     864     936 
   Size:        686      31      17     130      72

  Fragments arranged by size: 

                 686     130      72      31      17
 
SmlI C'TyrA_G

Cuts at:      0     232     936 
   Size:        232     704
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     169     183     272     739     936 
   Size:        169      14      89     467     197

  Fragments arranged by size: 

                 467     197     169      89      14
 
TatI w'GTAC_w

Cuts at:      0     483     936 
   Size:        483     453
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     825     857     936 
   Size:        825      32      79

  Fragments arranged by size: 

                 825      79      32
 

 Enzymes that do cut:

     AccI    AflII     ApaI    BanII     BbsI Bme1580I     BmrI    BsaXI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI    BssSI    BstYI
   Bsu36I   DraIII     DrdI     EaeI     EarI EcoO109I     FalI    Hin4I
     MmeI     MslI   PspOMI     PstI     SfcI     SmlI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI