Restriction map for
pFN21AE5967
(Linear) (Six-base) MAPSORT of: KIEE5967.seq Check: 5953 from: 1 to: 936
KIEE5967.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AflII C'TTAA_G
Cuts at: 0 387 486 700 936
Size: 387 99 214 236
Fragments arranged by size:
387 236 214 99
AleI CACnn'nnGTG
Cuts at: 0 731 936
Size: 731 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 803 936
Size: 803 133
ApoI r'AATT_y
Cuts at: 0 272 301 936
Size: 272 29 635
Fragments arranged by size:
635 272 29
BamHI G'GATC_C
Cuts at: 0 47 936
Size: 47 889
BanI G'GyrC_C
Cuts at: 0 843 936
Size: 843 93
BanII G_rGCy'C
Cuts at: 0 158 173 936
Size: 158 15 763
Fragments arranged by size:
763 158 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 243 529 670 936
Size: 243 286 141 266
Fragments arranged by size:
286 266 243 141
BciVI GTATCCnnnnn_n'
Cuts at: 0 540 936
Size: 540 396
BfrBI ATG'CAT
Cuts at: 0 780 936
Size: 780 156
BglI GCCn_nnn'nGGC
Cuts at: 0 196 936
Size: 196 740
BglII A'GATC_T
Cuts at: 0 902 936
Size: 902 34
Bme1580I G_kGCm'C
Cuts at: 0 758 936
Size: 758 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 936
Size: 281 655
BsaBI GATnn'nnATC
Cuts at: 0 46 936
Size: 46 890
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 936
Size: 216 720
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 936
Size: 111 825
BsiHKAI G_wGCw'C
Cuts at: 0 743 936
Size: 743 193
BsmI GAATG_Cn'
Cuts at: 0 455 936
Size: 455 481
Bsp1286I G_dGCh'C
Cuts at: 0 158 173 743 758 936
Size: 158 15 570 15 178
Fragments arranged by size:
570 178 158 15 15
BspHI T'CATG_A
Cuts at: 0 165 936
Size: 165 771
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 936
Size: 113 823
BsrDI GCAATG_nn'
Cuts at: 0 602 677 936
Size: 602 75 259
Fragments arranged by size:
602 259 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 558 936
Size: 558 378
BstYI r'GATC_y
Cuts at: 0 47 902 936
Size: 47 855 34
Fragments arranged by size:
855 47 34
BtgI C'CryG_G
Cuts at: 0 266 936
Size: 266 670
EcoO109I rG'GnC_Cy
Cuts at: 0 705 936
Size: 705 231
EcoRI G'AATT_C
Cuts at: 0 301 936
Size: 301 635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 46 936
Size: 46 890
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 308 340 936
Size: 308 32 596
Fragments arranged by size:
596 308 32
HindIII A'AGCT_T
Cuts at: 0 28 936
Size: 28 908
MslI CAynn'nnrTG
Cuts at: 0 325 731 936
Size: 325 406 205
Fragments arranged by size:
406 325 205
NcoI C'CATG_G
Cuts at: 0 266 936
Size: 266 670
NsiI A_TGCA'T
Cuts at: 0 782 936
Size: 782 154
NspI r_CATG'y
Cuts at: 0 67 936
Size: 67 869
PshAI GACnn'nnGTC
Cuts at: 0 583 936
Size: 583 353
PsiI TTA'TAA
Cuts at: 0 145 936
Size: 145 791
ScaI AGT'ACT
Cuts at: 0 341 936
Size: 341 595
SmlI C'TyrA_G
Cuts at: 0 296 387 486 700 936
Size: 296 91 99 214 236
Fragments arranged by size:
296 236 214 99 91
SspI AAT'ATT
Cuts at: 0 502 936
Size: 502 434
StyI C'CwwG_G
Cuts at: 0 266 936
Size: 266 670
TatI w'GTAC_w
Cuts at: 0 339 613 869 936
Size: 339 274 256 67
Fragments arranged by size:
339 274 256 67
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 936
Size: 765 171
Enzymes that do cut:
AflII AleI AlfI ApoI BamHI BanI BanII BbsI
BciVI BfrBI BglI BglII Bme1580I BpuEI BsaBI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstXI
BstYI BtgI EcoO109I EcoRI FalI Hin4I HindIII MslI
NcoI NsiI NspI PshAI PsiI ScaI SmlI SspI
StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BclI
BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TstI Tth111I XbaI XhoI XmaI XmnI
ZraI