Restriction map for
pFN21AE5964
(Linear) (Six-base) MAPSORT of: KIEE5964.seq Check: 6603 from: 1 to: 936
KIEE5964.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AccI GT'mk_AC
Cuts at: 0 842 936
Size: 842 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 702 719 936
Size: 180 522 17 217
Fragments arranged by size:
522 217 180 17
AflII C'TTAA_G
Cuts at: 0 493 936
Size: 493 443
AflIII A'CryG_T
Cuts at: 0 454 936
Size: 454 482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 928 936
Size: 928 8
AlwNI CAG_nnn'CTG
Cuts at: 0 211 725 936
Size: 211 514 211
Fragments arranged by size:
514 211 211
ApoI r'AATT_y
Cuts at: 0 28 936
Size: 28 908
AseI AT'TA_AT
Cuts at: 0 604 936
Size: 604 332
BbsI GAAGACnn'nnnn_
Cuts at: 0 433 708 936
Size: 433 275 228
Fragments arranged by size:
433 275 228
BciVI GTATCCnnnnn_n'
Cuts at: 0 265 936
Size: 265 671
BfrBI ATG'CAT
Cuts at: 0 644 936
Size: 644 292
BlpI GC'TnA_GC
Cuts at: 0 772 936
Size: 772 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 936
Size: 362 574
Bpu10I CC'TnA_GC
Cuts at: 0 10 936
Size: 10 926
BsaAI yAC'GTr
Cuts at: 0 274 457 936
Size: 274 183 479
Fragments arranged by size:
479 274 183
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 335 365 473 503 936
Size: 335 30 108 30 433
Fragments arranged by size:
433 335 108 30 30
BseYI C'CCAG_C
Cuts at: 0 158 936
Size: 158 778
BsiHKAI G_wGCw'C
Cuts at: 0 866 936
Size: 866 70
BsmI GAATG_Cn'
Cuts at: 0 53 525 936
Size: 53 472 411
Fragments arranged by size:
472 411 53
Bsp1286I G_dGCh'C
Cuts at: 0 866 936
Size: 866 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 854 936
Size: 854 82
BsrDI GCAATG_nn'
Cuts at: 0 113 936
Size: 113 823
BsrFI r'CCGG_y
Cuts at: 0 49 936
Size: 49 887
BstXI CCAn_nnnn'nTGG
Cuts at: 0 216 936
Size: 216 720
BstYI r'GATC_y
Cuts at: 0 83 936
Size: 83 853
BstZ17I GTA'TAC
Cuts at: 0 843 936
Size: 843 93
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 467 502 936
Size: 467 35 434
Fragments arranged by size:
467 434 35
EaeI y'GGCC_r
Cuts at: 0 377 936
Size: 377 559
EarI CTCTTCn'nnn_
Cuts at: 0 199 936
Size: 199 737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 362 702 719 936
Size: 180 182 340 17 217
Fragments arranged by size:
340 217 182 180 17
HincII GTy'rAC
Cuts at: 0 47 242 936
Size: 47 195 694
Fragments arranged by size:
694 195 47
MscI TGG'CCA
Cuts at: 0 379 936
Size: 379 557
MslI CAynn'nnrTG
Cuts at: 0 331 433 551 766 936
Size: 331 102 118 215 170
Fragments arranged by size:
331 215 170 118 102
NsiI A_TGCA'T
Cuts at: 0 646 936
Size: 646 290
NspI r_CATG'y
Cuts at: 0 572 771 936
Size: 572 199 165
Fragments arranged by size:
572 199 165
PmlI CAC'GTG
Cuts at: 0 274 457 936
Size: 274 183 479
Fragments arranged by size:
479 274 183
ScaI AGT'ACT
Cuts at: 0 467 936
Size: 467 469
SfcI C'TryA_G
Cuts at: 0 356 461 510 936
Size: 356 105 49 426
Fragments arranged by size:
426 356 105 49
SmlI C'TyrA_G
Cuts at: 0 493 936
Size: 493 443
SspI AAT'ATT
Cuts at: 0 473 936
Size: 473 463
StyI C'CwwG_G
Cuts at: 0 782 936
Size: 782 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 791 936
Size: 791 145
TatI w'GTAC_w
Cuts at: 0 465 936
Size: 465 471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 335 367 936
Size: 335 32 569
Fragments arranged by size:
569 335 32
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AlwNI ApoI AseI
BbsI BciVI BfrBI BlpI BpmI Bpu10I BsaAI BsaXI
BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstXI
BstYI BstZ17I CspCI EaeI EarI Eco57MI HincII MscI
MslI NsiI NspI PmlI ScaI SfcI SmlI SspI
StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BclI BglI
BglII Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI
BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI