Restriction map for
pFN21AE5963
(Linear) (Six-base) MAPSORT of: KIEE5963.seq Check: 9850 from: 1 to: 936
KIEE5963.orf
With 182 enzymes: SgfI *
December 5, 2014 13:16 ..
AflIII A'CryG_T
Cuts at: 0 98 936
Size: 98 838
AleI CACnn'nnGTG
Cuts at: 0 247 409 936
Size: 247 162 527
Fragments arranged by size:
527 247 162
AlwNI CAG_nnn'CTG
Cuts at: 0 755 936
Size: 755 181
ApoI r'AATT_y
Cuts at: 0 6 203 325 674 936
Size: 6 197 122 349 262
Fragments arranged by size:
349 262 197 122 6
BclI T'GATC_A
Cuts at: 0 122 455 516 936
Size: 122 333 61 420
Fragments arranged by size:
420 333 122 61
BlpI GC'TnA_GC
Cuts at: 0 290 936
Size: 290 646
BmgBI CAC'GTC
Cuts at: 0 215 936
Size: 215 721
BmrI ACTGGGnnnn_n'
Cuts at: 0 122 936
Size: 122 814
BsaBI GATnn'nnATC
Cuts at: 0 127 152 936
Size: 127 25 784
Fragments arranged by size:
784 127 25
BsaWI w'CCGG_w
Cuts at: 0 659 936
Size: 659 277
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 803 833 936
Size: 803 30 103
Fragments arranged by size:
803 103 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 708 830 936
Size: 708 122 106
Fragments arranged by size:
708 122 106
BsiHKAI G_wGCw'C
Cuts at: 0 406 824 936
Size: 406 418 112
Fragments arranged by size:
418 406 112
BsmI GAATG_Cn'
Cuts at: 0 224 936
Size: 224 712
Bsp1286I G_dGCh'C
Cuts at: 0 406 824 936
Size: 406 418 112
Fragments arranged by size:
418 406 112
BspEI T'CCGG_A
Cuts at: 0 659 936
Size: 659 277
BspHI T'CATG_A
Cuts at: 0 398 936
Size: 398 538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 54 936
Size: 54 882
BsrGI T'GTAC_A
Cuts at: 0 767 936
Size: 767 169
BstEII G'GTnAC_C
Cuts at: 0 618 936
Size: 618 318
BstYI r'GATC_y
Cuts at: 0 147 936
Size: 147 789
DraI TTT'AAA
Cuts at: 0 929 936
Size: 929 7
EaeI y'GGCC_r
Cuts at: 0 365 936
Size: 365 571
EarI CTCTTCn'nnn_
Cuts at: 0 386 540 936
Size: 386 154 396
Fragments arranged by size:
396 386 154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 815 909 936
Size: 815 94 27
Fragments arranged by size:
815 94 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 224 256 936
Size: 224 32 680
Fragments arranged by size:
680 224 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 936
Size: 470 466
MscI TGG'CCA
Cuts at: 0 367 936
Size: 367 569
MslI CAynn'nnrTG
Cuts at: 0 247 409 936
Size: 247 162 527
Fragments arranged by size:
527 247 162
MspA1I CmG'CkG
Cuts at: 0 359 936
Size: 359 577
NdeI CA'TA_TG
Cuts at: 0 725 936
Size: 725 211
NspI r_CATG'y
Cuts at: 0 102 936
Size: 102 834
PciI A'CATG_T
Cuts at: 0 98 936
Size: 98 838
PfoI T'CCnGG_A
Cuts at: 0 197 936
Size: 197 739
PsiI TTA'TAA
Cuts at: 0 275 936
Size: 275 661
PstI C_TGCA'G
Cuts at: 0 161 488 551 936
Size: 161 327 63 385
Fragments arranged by size:
385 327 161 63
SfcI C'TryA_G
Cuts at: 0 157 484 547 936
Size: 157 327 63 389
Fragments arranged by size:
389 327 157 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 269 936
Size: 269 667
TatI w'GTAC_w
Cuts at: 0 69 767 936
Size: 69 698 169
Fragments arranged by size:
698 169 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 803 835 936
Size: 803 32 101
Fragments arranged by size:
803 101 32
XbaI T'CTAG_A
Cuts at: 0 576 936
Size: 576 360
XmnI GAAnn'nnTTC
Cuts at: 0 267 853 936
Size: 267 586 83
Fragments arranged by size:
586 267 83
Enzymes that do cut:
AflIII AleI AlwNI ApoI BclI BlpI BmgBI BmrI
BsaBI BsaWI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrGI BstEII BstYI DraI EaeI EarI
FalI Hin4I MmeI MscI MslI MspA1I NdeI NspI
PciI PfoI PsiI PstI SfcI TaqII TatI TstI
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BglII Bme1580I
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BseYI BsgI BsiEI BsiWI BsmBI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI Tth111I XcmI XhoI
XmaI ZraI